Structural Maintenance of Chromosomes 1A (SMC1A) (Ser957) antibody

Details for Product No. ABIN151518
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Antigen
Synonyms smc1a, smc1l1, zgc:76902, im:7158706, SMC1L1, SMC1L2, NV15570, CDLS2, DXS423E, SB1.8, SMC1, SMC1alpha, SMCB, 5830426I24Rik, SMC-1A, Sb1.8, Smc1, Smc1alpha, Smc1l1, Smcb, mKIAA0178, smc1aa, smc1, xSMC1
Epitope
Ser957
(49), (14), (12), (12), (7), (7), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(156), (83), (33), (26), (26), (26), (24), (12), (2), (2), (1), (1), (1)
Host
Rabbit
(131), (26), (2), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1)
Application
Western Blotting (WB), Proximity Ligation Assay (PLA)
(122), (56), (43), (33), (30), (15), (15), (9), (7), (3), (1)
Pubmed 12 references available
Quantity 0.1 mL
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Catalog No. ABIN151518
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Immunogen Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 957 according to the numbering given in entry (NP_006297.2)
Cross-Reactivity (Details) Based on 100% sequence identity, this antibody is predicted to react with Bovine, Dog, Rabbit, Guinea pig, Pig, Panda, Orangutan, Rhesus Monkey, White-tufted-ear marmoset, Crab-eating macaque, European domestic ferret, African elephant, Naked mole rat,Northern white-cheeked gibbon.
Purification affinity purified
Alternative Name SMC1A / SMC1
Background Involved in chromosome cohesion during cell cycle and in DNA repair and is a centralcomponent of cohesin complex. The cohesin complex is required for the cohesion ofsister chromatids after DNA replication. The cohesin complex apparently forms a largeproteinaceous ring within which sister chromatids can be trapped. At anaphase, thecomplex is cleaved and dissociates from chromatin, allowing sister chromatids tosegregate. The cohesin complex may also play a role in spindle pole assembly duringmitosis. SMC1 is involved in DNA repair via its interaction with BRCA1 and its relatedphosphorylation by ATM, and works as a downstream effector in the ATM/NBS1 branchof S-phase checkpoint. Alternate Names: anti-SMC1L1-structural maintenance of chromosomes-1 like 1 antibody, anti-SMC-1antibody, anti-SMC 1 antibody.
Gene Symbol: SMC1A
Gene ID 8243
NCBI Accession NP_006297
Application Notes Recommended dilutions: Proximity Ligation Assay 1:1000-1:10000, Western Blot 1:200-1:2
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/Phosphate buffer, pH 7 to 8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
anti-Structural Maintenance of Chromosomes 1A (SMC1A) (Ser957) antibody Detection of Human Phospho Smc1 (ser957) by Western blot. Sample: Whole cell extracts from HeLa cells (30 mcg/lane) that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Irradiated lysate was then treated with calf intestinal phosphatase (CIP) or left untreated. ABIN151518 was used at 1:1000.
General Lukas, Falck, Bartkova et al.: "Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage." in: Nature cell biology, Vol. 5, Issue 3, pp. 255-60, 2003 (PubMed).

Mochan, Venere, DiTullio et al.: "53BP1 and NFBD1/MDC1-Nbs1 function in parallel interacting pathways activating ataxia-telangiectasia mutated (ATM) in response to DNA damage." in: Cancer research, Vol. 63, Issue 24, pp. 8586-91, 2003 (PubMed).

Wang, Wiltshire, Wang et al.: "ATM-dependent CHK2 activation induced by anticancer agent, irofulven." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 39584-92, 2004 (PubMed).

Kurz, Douglas, Lees-Miller: "Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species." in: The Journal of biological chemistry, Vol. 279, Issue 51, pp. 53272-81, 2004 (PubMed).

Goodarzi, Jonnalagadda, Douglas et al.: "Autophosphorylation of ataxia-telangiectasia mutated is regulated by protein phosphatase 2A." in: The EMBO journal, Vol. 23, Issue 22, pp. 4451-61, 2004 (PubMed).

Ismail, Nystroem, Nygren et al.: "Activation of ataxia telangiectasia mutated by DNA strand break-inducing agents correlates closely with the number of DNA double strand breaks." in: The Journal of biological chemistry, Vol. 280, Issue 6, pp. 4649-55, 2005 (PubMed).

Musio, Montagna, Mariani et al.: "SMC1 involvement in fragile site expression." in: Human molecular genetics, Vol. 14, Issue 4, pp. 525-33, 2005 (PubMed).

Bekker-Jensen, Lukas, Kitagawa et al.: "Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks." in: The Journal of cell biology, Vol. 173, Issue 2, pp. 195-206, 2006 (PubMed).

Biton, Dar, Mittelman et al.: "Nuclear ataxia-telangiectasia mutated (ATM) mediates the cellular response to DNA double strand breaks in human neuron-like cells." in: The Journal of biological chemistry, Vol. 281, Issue 25, pp. 17482-91, 2006 (PubMed).

Pereg, Lam, Teunisse et al.: "Differential roles of ATM- and Chk2-mediated phosphorylations of Hdmx in response to DNA damage." in: Molecular and cellular biology, Vol. 26, Issue 18, pp. 6819-31, 2006 (PubMed).

Biton, Gropp, Itsykson et al.: "ATM-mediated response to DNA double strand breaks in human neurons derived from stem cells." in: DNA repair, Vol. 6, Issue 1, pp. 128-34, 2007 (PubMed).

Pennarun, Granotier, Hoffschir et al.: "Role of ATM in the telomere response to the G-quadruplex ligand 360A." in: Nucleic acids research, Vol. 36, Issue 5, pp. 1741-54, 2008 (PubMed).

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