You are viewing an incomplete version of our website. Please click to reload the website as full version.

SMC1A antibody (Structural Maintenance of Chromosomes 1A) (pSer957)

Details for Product anti-SMC1A Antibody No. ABIN151518, Supplier: Log in to see
Antigen
  • 5830426I24Rik
  • CDLS2
  • DXS423E
  • im:7158706
  • mKIAA0178
  • NV15570
  • SB1.8
  • Sb1.8
  • SMC-1A
  • Smc1
  • SMC1
  • smc1
  • smc1a
  • smc1aa
  • Smc1alpha
  • SMC1alpha
  • Smc1l1
  • SMC1L1
  • smc1l1
  • SMC1L2
  • Smcb
  • SMCB
  • xSMC1
  • zgc:76902
Epitope
pSer957
49
37
16
15
15
13
7
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
217
101
41
4
2
2
2
2
1
1
1
Host
Rabbit
171
43
3
1
1
Clonality
Polyclonal
Conjugate
This SMC1A antibody is un-conjugated
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
173
81
60
39
31
29
26
26
25
5
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 957 according to the numbering given in entry (NP_006297.2)
Cross-Reactivity (Details) Based on 100% sequence identity, this antibody is predicted to react with Bovine, Dog, Rabbit, Guinea pig, Pig, Panda, Orangutan, Rhesus Monkey, White-tufted-ear marmoset, Crab-eating macaque, European domestic ferret, African elephant, Naked mole rat,Northern white-cheeked gibbon.
Purification Immunogen affinity purified
Alternative Name SMC1 (SMC1A Antibody Abstract)
Background Gene Symbol: SMC1A
Molecular Weight Theoretical MW: 143 kDa
UniProt Q14683
Research Area DNA/RNA, Cell Cycle, Chromatin and Nuclear Signaling
Application Notes Western Blot 1:200 - 1:2000, Immunoprecipitation 2 - 6 μg/mg of lysate, Proximity Ligation Assay 1:1000-1:10000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/phosphate buffer, pH 7 to 8
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-SMC1A antibody (Structural Maintenance of Chromosomes 1A) (pSer957) (ABIN151518) Detection of Human Phospho Smc1 (ser957) by Western blot. Sample: Whole cell extrac...
Proximity Ligation Assay (PLA) image for anti-SMC1A antibody (Structural Maintenance of Chromosomes 1A) (pSer957) (ABIN151518) Proximity Ligation Assay: SMC1 [p Ser957] Antibody [ABIN151518] - Secondary-conjugate...
Proximity Ligation Assay (PLA) image for anti-SMC1A antibody (Structural Maintenance of Chromosomes 1A) (pSer957) (ABIN151518) Proximity Ligation Assay: SMC1 [p Ser957] Antibody [ABIN151518] - Secondary-conjugate...
Proximity Ligation Assay (PLA) image for anti-SMC1A antibody (Structural Maintenance of Chromosomes 1A) (pSer957) (ABIN151518) Proximity Ligation Assay: SMC1 [p Ser957] Antibody [ABIN151518] - Secondary-conjugate...
Proximity Ligation Assay (PLA) image for anti-SMC1A antibody (Structural Maintenance of Chromosomes 1A) (pSer957) (ABIN151518) Proximity Ligation Assay: SMC1 [p Ser957] Antibody [ABIN151518] - Secondary-conjugate...
Proximity Ligation Assay (PLA) image for anti-SMC1A antibody (Structural Maintenance of Chromosomes 1A) (pSer957) (ABIN151518) Proximity Ligation Assay: SMC1 [p Ser957] Antibody [ABIN151518] - Secondary-conjugate...
Proximity Ligation Assay (PLA) image for anti-SMC1A antibody (Structural Maintenance of Chromosomes 1A) (pSer957) (ABIN151518) Proximity Ligation Assay: SMC1 [p Ser957] Antibody [ABIN151518] - Secondary-conjugate...
Product cited in: Valdez, Li, Murray, Ji, Liu, Popat, Champlin, Andersson: "Comparison of the cytotoxicity of cladribine and clofarabine when combined with fludarabine and busulfan in AML cells: Enhancement of cytotoxicity with epigenetic modulators." in: Experimental hematology, Vol. 43, Issue 6, pp. 448-61.e2, 2015 (PubMed). Method employed by authors: Western Blotting (WB)

Williamson, Kubota, Hamill, Klimowicz, Ye, Muzik, Dean, Tu, Gilley, Magliocco, McKay, Bebb, Lees-Miller: "Enhanced cytotoxicity of PARP inhibition in mantle cell lymphoma harbouring mutations in both ATM and p53." in: EMBO molecular medicine, Vol. 4, Issue 6, pp. 515-27, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Valdez, Nieto, Murray, Li, Wang, Champlin, Andersson: "Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines." in: Experimental hematology, Vol. 40, Issue 10, pp. 800-10, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Valdez, Murray, Nieto, Li, Wang, Champlin, Andersson: "Synergistic cytotoxicity of the DNA alkylating agent busulfan, nucleoside analogs and suberoylanilide hydroxamic acid in lymphoma cell lines." in: Leukemia & lymphoma, Vol. 53, Issue 5, pp. 973-81, 2012 (PubMed). (Sample species: Human).

Li, Chanda, Zhang, Choi, Chiang: "Glucose stimulates cholesterol 7alpha-hydroxylase gene transcription in human hepatocytes." in: Journal of lipid research, Vol. 51, Issue 4, pp. 832-42, 2010 (PubMed). (Sample species: Human).

Williamson, Muzik, Turhan, Zamò, OConnor, Bebb, Lees-Miller: "ATM deficiency sensitizes mantle cell lymphoma cells to poly(ADP-ribose) polymerase-1 inhibitors." in: Molecular cancer therapeutics, Vol. 9, Issue 2, pp. 347-57, 2010 (PubMed).

Valdez, Li, Murray, Champlin, Andersson: "The synergistic cytotoxicity of clofarabine, fludarabine and busulfan in AML cells involves ATM pathway activation and chromatin remodeling." in: Biochemical pharmacology, Vol. 81, Issue 2, pp. 222-32, 2010 (PubMed). (Sample species: Human). Further details: Western Blotting

Kodama, Otsubo, Hirota, Yokota, Enari, Taya: "Requirement of ATM for rapid p53 phosphorylation at Ser46 without Ser/Thr-Gln sequences." in: Molecular and cellular biology, Vol. 30, Issue 7, pp. 1620-33, 2010 (PubMed).

Pennarun, Granotier, Hoffschir, Mandine, Biard, Gauthier, Boussin: "Role of ATM in the telomere response to the G-quadruplex ligand 360A." in: Nucleic acids research, Vol. 36, Issue 5, pp. 1741-54, 2008 (PubMed).

Biton, Gropp, Itsykson, Pereg, Mittelman, Johe, Reubinoff, Shiloh: "ATM-mediated response to DNA double strand breaks in human neurons derived from stem cells." in: DNA repair, Vol. 6, Issue 1, pp. 128-34, 2007 (PubMed).

Bekker-Jensen, Lukas, Kitagawa, Melander, Kastan, Bartek, Lukas: "Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks." in: The Journal of cell biology, Vol. 173, Issue 2, pp. 195-206, 2006 (PubMed).

Biton, Dar, Mittelman, Pereg, Barzilai, Shiloh: "Nuclear ataxia-telangiectasia mutated (ATM) mediates the cellular response to DNA double strand breaks in human neuron-like cells." in: The Journal of biological chemistry, Vol. 281, Issue 25, pp. 17482-91, 2006 (PubMed).

Pereg, Lam, Teunisse, Biton, Meulmeester, Mittelman, Buscemi, Okamoto, Taya, Shiloh, Jochemsen: "Differential roles of ATM- and Chk2-mediated phosphorylations of Hdmx in response to DNA damage." in: Molecular and cellular biology, Vol. 26, Issue 18, pp. 6819-31, 2006 (PubMed).

Dar, Biton, Shiloh, Barzilai: "Analysis of the ataxia telangiectasia mutated-mediated DNA damage response in murine cerebellar neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 29, pp. 7767-74, 2006 (PubMed).

Musio, Montagna, Mariani, Tilenni, Focarelli, Brait, Indino, Benedetti, Chessa, Albertini, Ried, Vezzoni: "SMC1 involvement in fragile site expression." in: Human molecular genetics, Vol. 14, Issue 4, pp. 525-33, 2005 (PubMed).

Ismail, Nystroem, Nygren, Hammarsten: "Activation of ataxia telangiectasia mutated by DNA strand break-inducing agents correlates closely with the number of DNA double strand breaks." in: The Journal of biological chemistry, Vol. 280, Issue 6, pp. 4649-55, 2005 (PubMed).

Wang, Wiltshire, Wang, Mikell, Burks, Cunningham, Van Laar, Waters, Reed, Wang: "ATM-dependent CHK2 activation induced by anticancer agent, irofulven." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 39584-92, 2004 (PubMed).

Kurz, Douglas, Lees-Miller: "Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species." in: The Journal of biological chemistry, Vol. 279, Issue 51, pp. 53272-81, 2004 (PubMed).

Goodarzi, Jonnalagadda, Douglas, Young, Ye, Moorhead, Lees-Miller, Khanna: "Autophosphorylation of ataxia-telangiectasia mutated is regulated by protein phosphatase 2A." in: The EMBO journal, Vol. 23, Issue 22, pp. 4451-61, 2004 (PubMed).

Lukas, Falck, Bartkova, Bartek, Lukas: "Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage." in: Nature cell biology, Vol. 5, Issue 3, pp. 255-60, 2003 (PubMed).