Structural Maintenance of Chromosomes 1A (SMC1A) (pSer957) antibody

Antigen: Structural Maintenance of Chromosomes 1A (SMC1A)

Synonyms: SMC1, SMCB, CDLS2, SB1.8, SMC1L1, DXS423E, KIAA0178, MGC138332, SMC1alpha, DKFZp686L19178, SMC-1A, smc1a, smc1l1, zgc:76902, im:7158706, SMC1A, SMC1L2, bK268H5, FLJ43748, SMC1BETA, bK268H5.5, NV15570

Binding Site: pSer957

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN151518

Additional Information

Alternative name: SMC1A / SMC1

Gene ID: 8243

NCBI Acession: NP_0062972

Immunogen: Immunogen for this antibody was a phosphorylated synthetic peptide, whichrepresented a portion of human Structural Maintenance of Chromosomes 1 (GeneID8243) around serine 957 according to the numbering given in entry NP_006297.2.

Cross-Reactivity: Human

Description: Involved in chromosome cohesion during cell cycle and in DNA repair and is a centralcomponent of cohesin complex. The cohesin complex is required for the cohesion ofsister chromatids after DNA replication. The cohesin complex apparently forms a largeproteinaceous ring within which sister chromatids can be trapped. At anaphase, thecomplex is cleaved and dissociates from chromatin, allowing sister chromatids tosegregate. The cohesin complex may also play a role in spindle pole assembly duringmitosis. SMC1 is involved in DNA repair via its interaction with BRCA1 and its relatedphosphorylation by ATM, and works as a downstream effector in the ATM/NBS1 branchof S-phase checkpoint. Alternate Names: anti-SMC1L1-structural maintenance of chromosomes-1 like 1 antibody, anti-SMC-1antibody, anti-SMC 1 antibody.

Specificity: ABIN151518 reacts with human SMC1 phosphoSerine 957Phosphorylated:Ser957. Species Reactivity: Human SMC1 phosphoSerine 957.

Application Details

Application Notes: Suggested working dilutions: immunoprecipitation 2-6 ug/ml,Western Blot 1:200-1:2,000

Concentration: 1 mg/ml

Purification: Aff - Purified

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8. Preservative: 0.09% Sodium Azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Detection of Human Phospho Smc1 (ser957) by Western blot. Sample: Whole cell extracts from HeLa cells (30 mcg/lane) that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Irradiated lysate was then treated with calf intestinal phosphatase (CIP) or left untreated. ABIN151518 was used at 1:1000.

Publications

Lukas, Falck, Bartkova et al.: "Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage." in: Nature cell biology, Vol. 5, Issue 3, pp. 255-60, 2003 (PubMed).

Mochan, Venere, DiTullio et al.: "53BP1 and NFBD1/MDC1-Nbs1 function in parallel interacting pathways activating ataxia-telangiectasia mutated (ATM) in response to DNA damage." in: Cancer research, Vol. 63, Issue 24, pp. 8586-91, 2003 (PubMed).

Wang, Wiltshire, Wang et al.: "ATM-dependent CHK2 activation induced by anticancer agent, irofulven." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 39584-92, 2004 (PubMed).

Kurz, Douglas, Lees-Miller: "Doxorubicin activates ATM-dependent phosphorylation of multiple downstream targets in part through the generation of reactive oxygen species." in: The Journal of biological chemistry, Vol. 279, Issue 51, pp. 53272-81, 2004 (PubMed).

Goodarzi, Jonnalagadda, Douglas et al.: "Autophosphorylation of ataxia-telangiectasia mutated is regulated by protein phosphatase 2A." in: The EMBO journal, Vol. 23, Issue 22, pp. 4451-61, 2004 (PubMed).

Ismail, Nystroem, Nygren et al.: "Activation of ataxia telangiectasia mutated by DNA strand break-inducing agents correlates closely with the number of DNA double strand breaks." in: The Journal of biological chemistry, Vol. 280, Issue 6, pp. 4649-55, 2005 (PubMed).

Musio, Montagna, Mariani et al.: "SMC1 involvement in fragile site expression." in: Human molecular genetics, Vol. 14, Issue 4, pp. 525-33, 2005 (PubMed).

Bekker-Jensen, Lukas, Kitagawa et al.: "Spatial organization of the mammalian genome surveillance machinery in response to DNA strand breaks." in: The Journal of cell biology, Vol. 173, Issue 2, pp. 195-206, 2006 (PubMed).

Biton, Dar, Mittelman et al.: "Nuclear ataxia-telangiectasia mutated (ATM) mediates the cellular response to DNA double strand breaks in human neuron-like cells." in: The Journal of biological chemistry, Vol. 281, Issue 25, pp. 17482-91, 2006 (PubMed).

Pereg, Lam, Teunisse et al.: "Differential roles of ATM- and Chk2-mediated phosphorylations of Hdmx in response to DNA damage." in: Molecular and cellular biology, Vol. 26, Issue 18, pp. 6819-31, 2006 (PubMed).

Biton, Gropp, Itsykson et al.: "ATM-mediated response to DNA double strand breaks in human neurons derived from stem cells." in: DNA repair, Vol. 6, Issue 1, pp. 128-34, 2007 (PubMed).

Pennarun, Granotier, Hoffschir et al.: "Role of ATM in the telomere response to the G-quadruplex ligand 360A." in: Nucleic acids research, Vol. 36, Issue 5, pp. 1741-54, 2008 (PubMed).