ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2) antibody

Details for Product No. ABIN151597
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Antigen
Synonyms bcrp, ABCG2, ABC15, ABCP, BCRP, BCRP1, BMDP, CD338, CDw338, EST157481, GOUT1, MRX, MXR, MXR1, UAQTL1, AI428558, Bcrp1, ABCG3
Reactivity
Human
(82), (35), (12), (11), (2), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(46), (37), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (2)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(72), (29), (24), (24), (23), (16), (10), (9), (4), (3), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 0.25 mL
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Catalog No. ABIN151597
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Immunogen Fusion protein composed of E. Coli maltose binding protein and BCRP peptide
Clone BXP-21
Isotype IgG2a
Specificity This recognizes an internal epitope of human BCRP (70 kDa), a transmembrane half-transporter. The does not cross-react with the human MDR1, MRP1, and MRP2 gene products.
Cross-Reactivity (Details) Not yet tested in other species.
Purification Tissue culture supernatant
Alternative Name CD338 / ABCG2 / BCRP1
Background Tumor cells may display a multidrug resistant phenotype by overexpression ofATP-binding cassette transporters such as multidrug resistance (MDRI) p-glycoprotein,multidrug resistance protein 1 (MRP1), and breast cancer resistance protein (BCRP).Tumor cells can be intrinsically resistant to drugs or they can acquire resistance tostructurally and functionally unrelated drugs on drug exposure. This phenomenon isknown as multidrug resistance (MDR). In human tumor cells, several transporterproteins can be involved in MDR. These proteins, MDR1 P-gp (ABCB1), MRP1(ABCC1), MRP2 (ABCC2), MRP3 (ABCC3), and BCRP (ABCG2), belong to the ABCtransporter family. They act as efflux pumps, which result in decreased intracellularconcentrations of cytotoxic drugs.BCRP is a recently discovered half-transporter that probably acts as a homo- orheterodimer in transporting cytotoxic agents. The transporter molecule is capable oftransporting several anticancer drugs but has thus far been found mainly in MX-resistantcell lines. Alternate Names: Anti-ABC15 antibody, Anti-ABCG 2 antibody, Anti-ABCG2 antibody, Anti-ABCPantibody, Anti-ATP-binding cassette sub-family G member 2 antibody, Anti-BCRP1antibody, Anti-BMDP antibody, Anti-Breast cancer resistance protein antibody,Anti-CDw338 antibody, Anti-CDw338 antigen antibody, Anti-EST157481 antibody,Anti-MGC102821 antibody, Anti-Mitoxantrone resistance associated protein antibody,Anti-MRX antibody, Anti-MXR antibody, Anti-MXR1 antibody, Anti-Placenta-specificATP-binding cassette transporter antibody.
Gene Symbol: ABCG2
Gene ID 9429
Application Notes ICC: Using immunocytochemistry on acetone-fixed, frozen cytospin preparations. IHC: Using immunohistochemistry on acetone-fixed, frozen sections using rabbit anti-mouse IgG and a APAAP complex. The antibody can be used on formalin-fixed, paraffinembedded human tissues and tumors after pretreatment with 10 mM citric acid, pH 6.0, for 20 minutes. WB: Use at assay dependant dilution.
Recommended dilutions: Western Blot 1:100-1:2000, Immunohistochemistry-Paraffin 1:20-1:40, Immunohistochemistry-Frozen 1:20-1:40, Immunocytochemistry/Immunofluorescence 1:20-1:50, Immunohistochemistry 1:10-1:2000
Restrictions For Research Use only
Format Liquid
Buffer Supplied in serum-free medium containing 0.7 % BSA, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Doyle, Yang, Abruzzo et al.: "A multidrug resistance transporter from human MCF-7 breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 26, pp. 15665-70, 1999 (PubMed).

Maliepaard, Scheffer, Faneyte et al.: "Subcellular localization and distribution of the breast cancer resistance protein transporter in normal human tissues." in: Cancer research, Vol. 61, Issue 8, pp. 3458-64, 2001 (PubMed).

Ejendal, Diop, Schweiger et al.: "The nature of amino acid 482 of human ABCG2 affects substrate transport and ATP hydrolysis but not substrate binding." in: Protein science : a publication of the Protein Society, Vol. 15, Issue 7, pp. 1597-607, 2006 (PubMed).

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