ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2) antibody

Antigen: ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2)

Synonyms: MRX, MXR, ABCP, BCRP, BMDP, MXR1, ABC15, BCRP1, CD338, CDw338, EST157481, MGC102821, Bcrp1, AI428558, ABCG2, MGC127688, bcrp

Clonality: Monoclonal (BXP-21)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN151597

Additional Information

Alternative name: CD338 / ABCG2 / BCRP1

Gene ID: 9429

Immunogen: Fusion protein composed of E. Coli maltose binding protein and BCRP peptide [126amino acids, 271-396 AA of BCRP.

Cross-Reactivity: Human

Format: Supernatant

Isotype: IgG2a

Clone: BXP-21

Description: Tumor cells may display a multidrug resistant phenotype by overexpression ofATP-binding cassette transporters such as multidrug resistance (MDRI) p-glycoprotein,multidrug resistance protein 1 (MRP1), and breast cancer resistance protein (BCRP).Tumor cells can be intrinsically resistant to drugs or they can acquire resistance tostructurally and functionally unrelated drugs on drug exposure. This phenomenon isknown as multidrug resistance (MDR). In human tumor cells, several transporterproteins can be involved in MDR. These proteins, MDR1 P-gp (ABCB1), MRP1(ABCC1), MRP2 (ABCC2), MRP3 (ABCC3), and BCRP (ABCG2), belong to the ABCtransporter family. They act as efflux pumps, which result in decreased intracellularconcentrations of cytotoxic drugs.BCRP is a recently discovered half-transporter that probably acts as a homo- orheterodimer in transporting cytotoxic agents. The transporter molecule is capable oftransporting several anticancer drugs but has thus far been found mainly in MX-resistantcell lines. Alternate Names: Anti-ABC15 antibody, Anti-ABCG 2 antibody, Anti-ABCG2 antibody, Anti-ABCPantibody, Anti-ATP-binding cassette sub-family G member 2 antibody, Anti-BCRP1antibody, Anti-BMDP antibody, Anti-Breast cancer resistance protein antibody,Anti-CDw338 antibody, Anti-CDw338 antigen antibody, Anti-EST157481 antibody,Anti-MGC102821 antibody, Anti-Mitoxantrone resistance associated protein antibody,Anti-MRX antibody, Anti-MXR antibody, Anti-MXR1 antibody, Anti-Placenta-specificATP-binding cassette transporter antibody.

Specificity: This antibody recognizes an internal epitope of human BCRP (70 kDa), atransmembrane half-transporter. The antibody does not cross-react with the humanMDR1, MRP1, and MRP2 gene products. Species Reactivity: Cross-reacts with Human. Not yet tested in other species. Localization: Plasma membrane, integral membrane protein.

Application Details

Application Notes: ICC: Using immunocytochemistry on acetone-fixed, frozen cytospin preparations.IHC: Using immunohistochemistry on acetone-fixed, frozen sections usingrabbit anti-mouse IgG and a APAAP complex. The antibody can be used onformalin-fixed, paraffinembedded human tissues and tumors after pretreatment with 10mM citric acid, pH 6.0, for 20 minutes.WB: Use at assay dependant dilution.

Concentration: 0.25 mg/ml

Purification: Supernatant

Buffer: PBS with 0.7% BSA. Preservative: Sodium Azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Publications

Doyle, Yang, Abruzzo et al.: "A multidrug resistance transporter from human MCF-7 breast cancer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 26, pp. 15665-70, 1999 (PubMed).

Maliepaard, Scheffer, Faneyte et al.: "Subcellular localization and distribution of the breast cancer resistance protein transporter in normal human tissues." in: Cancer research, Vol. 61, Issue 8, pp. 3458-64, 2001 (PubMed).

Ejendal, Diop, Schweiger et al.: "The nature of amino acid 482 of human ABCG2 affects substrate transport and ATP hydrolysis but not substrate binding." in: Protein science : a publication of the Protein Society, Vol. 15, Issue 7, pp. 1597-607, 2006 (PubMed).