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RNF20 antibody (Ring Finger Protein 20) (AA 1-50)

Details for Product anti-RNF20 Antibody No. ABIN151665, Supplier: Log in to see
Antigen
  • 4833430L21Rik
  • AW540162
  • BRE1
  • BRE1A
  • C79397
  • hBRE1
  • mKIAA4116
Epitope
AA 1-50
23
15
5
3
3
3
2
2
2
2
2
1
Reactivity
Human, Mouse (Murine)
72
28
25
2
2
2
Host
Rabbit
63
9
Clonality
Polyclonal
Conjugate
This RNF20 antibody is un-conjugated
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blotting (WB)
55
27
12
12
9
6
2
1
1
Supplier
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Immunogen The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254).
Cross-Reactivity (Details) Mouse reactivity reported in the scientific literature (PMID: 23760504). Based on 100% sequence identity, this antibody is predicted to react with Rat, Dog, Rabbit, Pig, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Tasmanian devil, White-tufted-ear marmoset, Crab-eating macaque, European domestic ferret, Chinese hamster,Northern white-cheeked gibbon.
Purification Immunogen affinity purified
Alternative Name RNF20 (RNF20 Antibody Abstract)
Background Gene Symbol: RNF20
Gene ID 56254
UniProt Q5VTR2
Research Area Proteolysis / Ubiquitin, Ion Channels, Chromatin and Nuclear Signaling
Application Notes Western Blot 1:2000-1:10000, Chromatin Immunoprecipitation 1:10-1:500, Immunoprecipitation 2-10 μg/mg lysate
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/phosphate buffer, pH 7 to 8
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-RNF20 antibody (Ring Finger Protein 20) (AA 1-50) (ABIN151665) anti-Ring Finger Protein 20 (RNF20) (AA 1-50) antibody
Western Blotting (WB) image for anti-RNF20 antibody (Ring Finger Protein 20) (AA 1-50) (ABIN151665) Western Blot: RNF20 Antibody [ABIN151665] - Whole cell lysate HeLa, 293T, and mouse N...
Western Blotting (WB) image for anti-RNF20 antibody (Ring Finger Protein 20) (AA 1-50) (ABIN151665) Western Blot: RNF20 Antibody [ABIN151665] - WB analysis of RNF20 in HeLa whole cell l...
Product cited in: Chen, Jing, Kang, Yang, Wang, Zhang, Zhang, Chen, Chang, Yang, Sun: "Histone H2B monoubiquitination is a critical epigenetic switch for the regulation of autophagy." in: Nucleic acids research, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC)

Wu, Chen, Jing, Wang, Men, Zhan, Wang, Gan, Huang, Xie, Mi, Yu, Yu, Chen, Chang, Cai, Chen: "Microarray Analysis Reveals Potential Biological Functions of Histone H2B Monoubiquitination." in: PLoS ONE, Vol. 10, Issue 7, pp. e0133444, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Wang, Kawaoka, Yu, Shi, Ni, Yang, Zhu, Roeder, Vakoc: "Histone H2B ubiquitin ligase RNF20 is required for MLL-rearranged leukemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 10, pp. 3901-6, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Sussman, Stanek, Esteso, Gearhart, Knudsen, McMahon: "The epigenetic modifier ubiquitin-specific protease 22 (USP22) regulates embryonic stem cell differentiation via transcriptional repression of sex-determining region Y-box 2 (SOX2)." in: The Journal of biological chemistry, Vol. 288, Issue 33, pp. 24234-46, 2013 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Hahn, Dickson, Jackson, Clarkson, Gill, Marsh: "The tumor suppressor CDC73 interacts with the ring finger proteins RNF20 and RNF40 and is required for the maintenance of histone 2B monoubiquitination." in: Human molecular genetics, Vol. 21, Issue 3, pp. 559-68, 2012 (PubMed).

Jääskeläinen, Makkonen, Visakorpi, Kim, Roeder, Palvimo: "Histone H2B ubiquitin ligases RNF20 and RNF40 in androgen signaling and prostate cancer cell growth." in: Molecular and cellular endocrinology, Vol. 350, Issue 1, pp. 87-98, 2012 (PubMed).

Arora, Zhang, Heine, Ozer, Liu, Huang, Parvin: "Promoters active in interphase are bookmarked during mitosis by ubiquitination." in: Nucleic acids research, Vol. 40, Issue 20, pp. 10187-202, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Kim, Roeder: "Nucleosomal H2B ubiquitylation with purified factors." in: Methods (San Diego, Calif.), Vol. 54, Issue 3, pp. 331-8, 2011 (PubMed).

Taniue, Oda, Hayashi, Okuno, Akiyama: "A member of the ETS family, EHF, and the ATPase RUVBL1 inhibit p53-mediated apoptosis." in: EMBO reports, Vol. 12, Issue 7, pp. 682-9, 2011 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Buro, Chipumuro, Henriksen et al.: "Menin and RNF20 recruitment is associated with dynamic histone modifications that regulate signal transducer and activator of transcription 1 (STAT1)-activated transcription of the interferon ..." in: Epigenetics & chromatin, Vol. 3, Issue 1, pp. 16, 2010 (PubMed). (Sample species: Human). Further details: Western Blotting

Wu, Huen, Lu, Ye, Dou, Ljungman, Chen, Yu: "Histone ubiquitination associates with BRCA1-dependent DNA damage response." in: Molecular and cellular biology, Vol. 29, Issue 3, pp. 849-60, 2009 (PubMed).

Shema, Tirosh, Aylon, Huang, Ye, Moskovits, Raver-Shapira, Minsky, Pirngruber, Tarcic, Hublarova, Moyal, Gana-Weisz, Shiloh, Yarden, Johnsen, Vojtesek, Berger, Oren: "The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression." in: Genes & development, Vol. 22, Issue 19, pp. 2664-76, 2008 (PubMed).