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Ring Finger Protein 20 (RNF20) antibody

Details for Product No. ABIN151665, Supplier: Login to see
Antigen
  • 4833430L21Rik
  • AW540162
  • C79397
  • mKIAA4116
  • BRE1
  • BRE1A
  • hBRE1
Reactivity
Human
67
26
24
2
2
2
Host
Rabbit
59
8
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western Blotting (WB)
51
27
12
11
8
7
2
1
Supplier
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Immunogen The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID 56254).
Cross-Reactivity (Details) Mouse reactivity reported in the scientific literature (PMID: 23760504). Based on 100% sequence identity, this antibody is predicted to react with Rat, Dog, Rabbit, Pig, Panda, Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, Tasmanian devil, White-tufted-ear marmoset, Crab-eating macaque, European domestic ferret, Chinese hamster,Northern white-cheeked gibbon.
Purification affinity purified
Alternative Name BRE1A / RNF20 (RNF20 Antibody Abstract)
Background The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. YeastBRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and themethylation of histone H3. Alternate Names: anti-RNF20 antibody, anti-RNF-20 antibody, anti-Ring Finger 20 antibody, anti-BRE1Aantibody, anti-Homolog of S. cerevisiae BRE1 antibody.
Gene Symbol: RNF20
Gene ID 56254
NCBI Accession NP_062538
Pathways
Application Notes Recommended dilutions: Chromatin Immunoprecipitation 1:10-1:500, Immunoprecipitation 2-5 µg/mg lysate, Western Blot 1:2000-1:10000
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/Phosphate buffer, pH 7 to 8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-Ring Finger Protein 20 (RNF20) antibody (ABIN151665) anti-Ring Finger Protein 20 (RNF20) antibody
Product cited in: Shema, Tirosh, Aylon et al.: "The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression." in: Genes & development, Vol. 22, Issue 19, pp. 2664-76, 2008 (PubMed).