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Ring Finger Protein 20 (RNF20) antibody

Antigen

Ring Finger Protein 20 (RNF20)
Synonyms BRE1, BRE1A, hBRE1, FLJ11189, FLJ20382, KIAA2779, MGC129667, MGC129668
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunoprecipitation (IP), Western Blotting (WB)
1 reference available
Catalog no. ABIN151665
Quantity 0.1 mg  (1 mg/ml)  (Variants)
Price Product not available in this region.
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anti-Ring Finger Protein 20 (RNF20) antibody

Additional Information
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Alternative name BRE1A / RNF20
Gene ID 56254
NCBI Acession NP_0625385
Immunogen The epitope recognized by this antibody maps to a region between residue 1 and 50 ofhuman Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID56254).
Cross-Reactivity Human
Description The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. YeastBRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and themethylation of histone H3. Alternate Names: anti-RNF20 antibody, anti-RNF-20 antibody, anti-Ring Finger 20 antibody, anti-BRE1Aantibody, anti-Homolog of S. cerevisiae BRE1 antibody.
Specificity Species Reactivity: Human. Initial blots performed on lysate from NIH3T3 cells failed to provide evidencethat this antibody reacts with the Mouse ortholog.

Application Details
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Application Notes Optimal working dilutions should be determined experimentally by the investigator.Prepare working dilution immediately before use.Suggested starting dilutions are as follows:*Western Blot: 1:2,000 to 1:10,000, Immunoprecipitation: 2 to 5 mcg/mg lysateImmunohistochemistry: not testedImmunocytochemistry: not tested*Investigator should determine optimal working conditions and dilutions.
Concentration 1 mg/ml
Purification Aff - Purified
Buffer Tris-citrate/phosphate buffer, pH 7 to 8. Preservative: 0.09% Sodium Azide.
Storage Store at 4C. Do not freeze.
Restrictions For Research Use only

Publications
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Shema, Tirosh, Aylon et al.: "The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression." in: Genes & development, Vol. 22, Issue 19, pp. 2664-76, 2008 (PubMed).

Alternatives
Application Details Publications Images Variants back to top

Alternatives for antigen "Ring Finger Protein 20 (RNF20)", type "Antibodies"
Hosts Rabbit (31), Mouse (1)
Reactivities Human (29), Mouse (Murine) (22), Rat (Rattus) (22), Chicken (20), Cow (Bovine) (2), Dog (Canine) (2)
Applications Immunofluorescence (IF) (16), Western Blotting (WB) (16), ELISA (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes N-Term (2), AA 1-50 (1), AA 125-175 (1), Internal Region (1)