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Details for Product No. ABIN151665

Ring Finger Protein 20 (RNF20) antibody

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Antigen
Synonyms BRE1, BRE1A, hBRE1, 4833430L21Rik, AW540162, C79397, mKIAA4116
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 1 reference available
Catalog no. ABIN151665
Quantity 0.1 mg
Price
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Immunogen The epitope recognized by this antibody maps to a region between residue 1 and 50 ofhuman Ring Finger 20 using the numbering given in entry NP_062538.5 (GeneID56254).
Specificity Species Reactivity: Human. Initial blots performed on lysate from NIH3T3 cells failed to provide evidencethat this antibody reacts with the Mouse ortholog.
Cross-Reactivity Human
Purification Aff - Purified
Alternative Name BRE1A / RNF20
Background The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. YeastBRE1 is a ubiquitin ligase required for the ubiquitination of histone H2B and themethylation of histone H3. Alternate Names: anti-RNF20 antibody, anti-RNF-20 antibody, anti-Ring Finger 20 antibody, anti-BRE1Aantibody, anti-Homolog of S. cerevisiae BRE1 antibody.
Gene ID 56254
NCBI Accession NP_0625385
Application Notes Optimal working dilutions should be determined experimentally by the investigator.Prepare working dilution immediately before use.Suggested starting dilutions are as follows:*Western Blot: 1:2,000 to 1:10,000, Immunoprecipitation: 2 to 5 mcg/mg lysateImmunohistochemistry: not testedImmunocytochemistry: not tested*Investigator should determine optimal working conditions and dilutions.
Restrictions For Research Use only
Concentration 1 mg/ml
Buffer Tris-citrate/phosphate buffer, pH 7 to 8. Preservative: 0.09% Sodium Azide.
Preservative Sodium azide
Storage 4 °C
Shema, Tirosh, Aylon et al.: "The histone H2B-specific ubiquitin ligase RNF20/hBRE1 acts as a putative tumor suppressor through selective regulation of gene expression." in: Genes & development, Vol. 22, Issue 19, pp. 2664-76, 2008 (PubMed).

Alternatives for antigen "Ring Finger Protein 20 (RNF20)", type "Antibodies"
Hosts (30), (5)
Reactivities (33), (17), (16), (14), (14), (14), (12)
Applications (25), (12), (10), (4), (4), (4), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (3), (2), (2), (1), (1), (1), (1), (1)
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