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DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody

Details for Product No. ABIN151732, Supplier: Log in to see
Antigen
  • ADCADN
  • AIM
  • cb983
  • cb1075
  • CXXC9
  • Cxxc9
  • DDM2
  • DECREASED DNA METHYLATION 2
  • dmnt1
  • DMT01
  • DMT1
  • DNA METHYLTRANSFERASE
  • DNA METHYLTRANSFERASE 01
  • DNA METHYLTRANSFERASE 1
  • Dnmt
  • DNMT
  • Dnmt1o
  • fb05h01
  • HSN1E
  • m.MmuI
  • MCMT
  • Met-1
  • Met1
  • MET2
  • methyltransferase 1
  • METHYLTRANSFERASE 2
  • METHYLTRANSFERASE I
  • METI
  • MommeD2
  • mta
  • MTase
  • wu:fb05h01
Epitope
N-Term
63
42
35
27
22
21
20
19
14
14
14
12
9
9
9
7
7
7
6
6
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
408
131
64
57
36
23
5
5
3
3
1
1
1
1
1
1
1
1
Host
Rabbit
254
130
20
8
3
Clonality
Polyclonal
Conjugate
Un-conjugated
24
23
21
19
18
18
8
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
271
134
124
123
101
73
55
49
23
20
10
7
3
2
2
2
2
Supplier
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Immunogen Contained within amino acids 1-125 of the N-terminus of human Dnmt1 [UniProt# P26358]
Purification Unpurified
Alternative Name DNMT1 (DNMT1 Antibody Abstract)
Background Gene Symbol: DNMT1
Molecular Weight Theoretical MW: 190 kDa
Gene ID 1786
UniProt P26358
Research Area DNA/RNA
Application Notes Western Blot 1:1000, Simple Western 1:100, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-Paraffin Protocol for Dnmt1 Antibody Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunocytochemistry/Immunofluorescence Protocol for Dnmt1 Antibody Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody (ABIN151732) Western blot analysis using ABIN151732 to detect DNMT1 levels in T24 cells. Lane 1:50...
Simple Western (SimWes) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody (ABIN151732) Simple Western: DNMT1 Antibody [ABIN151732] - Simple Western lane view shows a specif...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody (ABIN151732) Immunohistochemistry-Paraffin: DNMT1 Antibody [ABIN151732] - IHC staining of Dnmt1 in...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody (ABIN151732) Immunohistochemistry-Paraffin: DNMT1 Antibody [ABIN151732] - IHC staining of Dnmt1 in...
Immunofluorescence (IF) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody (ABIN151732) Immunocytochemistry/Immunofluorescence: DNMT1 Antibody [ABIN151732] - The Dnmt1 antib...
Product cited in: Cui, Irudayaraj: "Dissecting the behavior and function of MBD3 in DNA methylation homeostasis by single-molecule spectroscopy and microscopy." in: Nucleic acids research, Vol. 43, Issue 6, pp. 3046-55, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Vispé, Deroide, Davoine, Desjobert, Lestienne, Fournier, Novosad, Bréand, Besse, Busato, Tost, De Vries, Cussac, Riond, Arimondo: "Consequences of combining siRNA-mediated DNA methyltransferase 1 depletion with 5-aza-2'-deoxycytidine in human leukemic KG1 cells." in: Oncotarget, Vol. 6, Issue 17, pp. 15265-82, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Chang, Pirozzi, Wen, Chung, Chiu, Errico, Luongo, Lombardi, Ferrone: "Multiple structural and epigenetic defects in the human leukocyte antigen class I antigen presentation pathway in a recurrent metastatic melanoma following immunotherapy." in: The Journal of biological chemistry, Vol. 290, Issue 44, pp. 26562-75, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Wong, Gertz, Chestnut, Martin: "Mitochondrial DNMT3A and DNA methylation in skeletal muscle and CNS of transgenic mouse models of ALS." in: Frontiers in cellular neuroscience, Vol. 7, pp. 279, 2014 (PubMed). (Sample species: Mouse (Murine)).

Gros, Chauvigné, Poulet, Menon, Ausseil, Dufau, Arimondo: "Development of a universal radioactive DNA methyltransferase inhibition test for high-throughput screening and mechanistic studies." in: Nucleic acids research, Vol. 41, Issue 19, pp. e185, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Lai, Mav, Shah, Grimm, Phadke, Hatzi, Melnick, Geigerman, Sobol, Jaye, Wade: "DNA methylation profiling in human B cells reveals immune regulatory elements and epigenetic plasticity at Alu elements during B-cell activation." in: Genome research, Vol. 23, Issue 12, pp. 2030-41, 2013 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Selvakumar, Gjidoda, Hovde, Henry: "Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases." in: The Journal of biological chemistry, Vol. 287, Issue 10, pp. 7039-50, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Yin, Zhang, Lv, He, Wu, Liu, Ding, Dong, Qu, Xu, Zhu, Huang: "Insufficient maintenance DNA methylation is associated with abnormal embryonic development." in: BMC medicine, Vol. 10, pp. 26, 2012 (PubMed). Further details: Western Blotting,Immunohistochemistry

Chang, Chu, Peng, Mao, Shan, Hsu, Lin, Tsai, Yu, Chang: "Krüppel-like factor 10 expression as a prognostic indicator for pancreatic adenocarcinoma." in: The American journal of pathology, Vol. 181, Issue 2, pp. 423-30, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Al-Salihi, Yu, Burnett, Alexander, Samlowski, Fitzpatrick: "The depletion of DNA methyltransferase-1 and the epigenetic effects of 5-aza-2'deoxycytidine (decitabine) are differentially regulated by cell cycle progression." in: Epigenetics, Vol. 6, Issue 8, pp. 1021-8, 2011 (PubMed).

Kaminskyy, Surova, Vaculova, Zhivotovsky: "Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL." in: Carcinogenesis, Vol. 32, Issue 10, pp. 1450-8, 2011 (PubMed).

Shaknovich, Cerchietti, Tsikitas, Kormaksson, De, Figueroa, Ballon, Yang, Weinhold, Reimers, Clozel, Luttrop, Ekstrom, Frank, Vasanthakumar, Godley, Michor, Elemento, Melnick: "DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation." in: Blood, Vol. 118, Issue 13, pp. 3559-69, 2011 (PubMed).

Kinney, Moser, Pascual, Greally, Foster, Karpf: "Opposing roles of Dnmt1 in early- and late-stage murine prostate cancer." in: Molecular and cellular biology, Vol. 30, Issue 17, pp. 4159-74, 2010 (PubMed). (Sample species: Mouse (Murine)).

Reams, Brewer, Guldin: "Remote sensing alone is insufficient for quantifying changes in forest cover." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 38, pp. E145; author reply E146, 2010 (PubMed).

Morey Kinney, Smiraglia, James, Moser, Foster, Karpf et al.: "Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate ..." in: Molecular cancer research : MCR, Vol. 6, Issue 8, pp. 1365-74, 2008 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Estève, Chin, Smallwood, Feehery, Gangisetty, Karpf, Carey, Pradhan: "Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication." in: Genes & development, Vol. 20, Issue 22, pp. 3089-103, 2006 (PubMed).

James, Link, Karpf: "Epigenetic regulation of X-linked cancer/germline antigen genes by DNMT1 and DNMT3b." in: Oncogene, Vol. 25, Issue 52, pp. 6975-85, 2006 (PubMed). (Sample species: Human).

Robert, Morin, Beaulieu, Gauthier, Chute, Barsalou, MacLeod: "DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells." in: Nature genetics, Vol. 33, Issue 1, pp. 61-5, 2003 (PubMed). (Sample species: Human). Further details: Western Blotting

Bachman, Park, Rhee, Rajagopalan, Herman, Baylin, Kinzler, Vogelstein: "Histone modifications and silencing prior to DNA methylation of a tumor suppressor gene." in: Cancer cell, Vol. 3, Issue 1, pp. 89-95, 2003 (PubMed).

Yang, Seto: "Collaborative spirit of histone deacetylases in regulating chromatin structure and gene expression." in: Current opinion in genetics & development, Vol. 13, Issue 2, pp. 143-53, 2003 (PubMed).