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DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody

Details for Product No. ABIN151732, Supplier: Log in to see
Antigen
  • cb1075
  • cb983
  • dmnt1
  • fb05h01
  • mta
  • wu:fb05h01
  • ADCADN
  • AIM
  • CXXC9
  • DNMT
  • HSN1E
  • MCMT
  • DDM2
  • DECREASED DNA METHYLATION 2
  • DMT01
  • DMT1
  • DNA METHYLTRANSFERASE
  • DNA METHYLTRANSFERASE 01
  • DNA METHYLTRANSFERASE 1
  • MET2
  • METHYLTRANSFERASE 2
  • METHYLTRANSFERASE I
  • METI
  • methyltransferase 1
  • Cxxc9
  • Dnmt
  • Dnmt1o
  • MTase
  • Met-1
  • Met1
  • MommeD2
  • m.MmuI
  • DNA (cytosine-5-)-methyltransferase 1
  • DNA (cytosine-5)-methyltransferase 1
  • DNA methyltransferase (cytosine-5) 1
  • DNMT1
  • dnmt1
  • Dnmt1
  • MET1
Epitope
N-Term
66
43
36
22
21
20
19
16
15
15
15
11
8
7
7
7
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
430
182
95
73
40
26
8
8
6
5
5
3
2
2
2
1
1
1
1
Host
Rabbit
267
137
20
8
3
Clonality
Polyclonal
Conjugate
Un-conjugated
24
24
21
19
18
18
6
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
3
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
284
139
118
112
89
73
53
52
50
38
36
16
6
5
2
2
2
2
2
1
Options
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Immunogen Contained within amino acids 1-125 of the N-terminus of human Dnmt1 [UniProt# P26358]
Purification Unpurified
Alternative Name DNMT1 (DNMT1 Antibody Abstract)
Background Gene Symbol: DNMT1
Molecular Weight Theoretical MW: 190 kDa
Gene ID 1786
UniProt P26358
Research Area DNA/RNA
Application Notes Western Blot 1:1000, Simple Western 1:100, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100This Dnmt1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western Blot, where a band can be seen at approx. 190 kDa. NOTE: This antibody does not neutralize the enzymatic activity of its corresponding methyltransferase. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-Paraffin Protocol for Dnmt1 Antibody Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunocytochemistry/Immunofluorescence Protocol for Dnmt1 Antibody Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Buffer Whole antisera
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody (ABIN151732) Western blot analysis using ABIN151732 to detect DNMT1 levels in T24 cells. Lane 1:50...
Simple Western (SimWes) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody (ABIN151732) Simple Western: DNMT1 Antibody [ABIN151732] - Simple Western lane view shows a specif...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody (ABIN151732) Immunohistochemistry-Paraffin: DNMT1 Antibody [ABIN151732] - IHC staining of Dnmt1 in...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody (ABIN151732) Immunohistochemistry-Paraffin: DNMT1 Antibody [ABIN151732] - IHC staining of Dnmt1 in...
Immunofluorescence (IF) image for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (N-Term) antibody (ABIN151732) Immunocytochemistry/Immunofluorescence: DNMT1 Antibody [ABIN151732] - The Dnmt1 antib...
Product cited in: Cui, Irudayaraj: "Dissecting the behavior and function of MBD3 in DNA methylation homeostasis by single-molecule spectroscopy and microscopy." in: Nucleic acids research, Vol. 43, Issue 6, pp. 3046-55, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Vispé, Deroide, Davoine, Desjobert, Lestienne, Fournier, Novosad, Bréand, Besse, Busato, Tost, De Vries, Cussac, Riond, Arimondo: "Consequences of combining siRNA-mediated DNA methyltransferase 1 depletion with 5-aza-2'-deoxycytidine in human leukemic KG1 cells." in: Oncotarget, Vol. 6, Issue 17, pp. 15265-82, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Chang, Pirozzi, Wen, Chung, Chiu, Errico, Luongo, Lombardi, Ferrone: "Multiple structural and epigenetic defects in the human leukocyte antigen class I antigen presentation pathway in a recurrent metastatic melanoma following immunotherapy." in: The Journal of biological chemistry, Vol. 290, Issue 44, pp. 26562-75, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Wong, Gertz, Chestnut, Martin: "Mitochondrial DNMT3A and DNA methylation in skeletal muscle and CNS of transgenic mouse models of ALS." in: Frontiers in cellular neuroscience, Vol. 7, pp. 279, 2014 (PubMed). (Sample species: Mouse (Murine)).

Gros, Chauvigné, Poulet, Menon, Ausseil, Dufau, Arimondo: "Development of a universal radioactive DNA methyltransferase inhibition test for high-throughput screening and mechanistic studies." in: Nucleic acids research, Vol. 41, Issue 19, pp. e185, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Lai, Mav, Shah, Grimm, Phadke, Hatzi, Melnick, Geigerman, Sobol, Jaye, Wade: "DNA methylation profiling in human B cells reveals immune regulatory elements and epigenetic plasticity at Alu elements during B-cell activation." in: Genome research, Vol. 23, Issue 12, pp. 2030-41, 2013 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Selvakumar, Gjidoda, Hovde, Henry: "Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases." in: The Journal of biological chemistry, Vol. 287, Issue 10, pp. 7039-50, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Yin, Zhang, Lv, He, Wu, Liu, Ding, Dong, Qu, Xu, Zhu, Huang: "Insufficient maintenance DNA methylation is associated with abnormal embryonic development." in: BMC medicine, Vol. 10, pp. 26, 2012 (PubMed). Further details: Western Blotting,Immunohistochemistry

Chang, Chu, Peng, Mao, Shan, Hsu, Lin, Tsai, Yu, Chang: "Krüppel-like factor 10 expression as a prognostic indicator for pancreatic adenocarcinoma." in: The American journal of pathology, Vol. 181, Issue 2, pp. 423-30, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Al-Salihi, Yu, Burnett, Alexander, Samlowski, Fitzpatrick: "The depletion of DNA methyltransferase-1 and the epigenetic effects of 5-aza-2'deoxycytidine (decitabine) are differentially regulated by cell cycle progression." in: Epigenetics, Vol. 6, Issue 8, pp. 1021-8, 2011 (PubMed).

Kaminskyy, Surova, Vaculova, Zhivotovsky: "Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL." in: Carcinogenesis, Vol. 32, Issue 10, pp. 1450-8, 2011 (PubMed).

Shaknovich, Cerchietti, Tsikitas, Kormaksson, De, Figueroa, Ballon, Yang, Weinhold, Reimers, Clozel, Luttrop, Ekstrom, Frank, Vasanthakumar, Godley, Michor, Elemento, Melnick: "DNA methyltransferase 1 and DNA methylation patterning contribute to germinal center B-cell differentiation." in: Blood, Vol. 118, Issue 13, pp. 3559-69, 2011 (PubMed).

Kinney, Moser, Pascual, Greally, Foster, Karpf: "Opposing roles of Dnmt1 in early- and late-stage murine prostate cancer." in: Molecular and cellular biology, Vol. 30, Issue 17, pp. 4159-74, 2010 (PubMed). (Sample species: Mouse (Murine)).

Reams, Brewer, Guldin: "Remote sensing alone is insufficient for quantifying changes in forest cover." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 38, pp. E145; author reply E146, 2010 (PubMed).

Morey Kinney, Smiraglia, James, Moser, Foster, Karpf et al.: "Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate ..." in: Molecular cancer research : MCR, Vol. 6, Issue 8, pp. 1365-74, 2008 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Estève, Chin, Smallwood, Feehery, Gangisetty, Karpf, Carey, Pradhan: "Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication." in: Genes & development, Vol. 20, Issue 22, pp. 3089-103, 2006 (PubMed).

James, Link, Karpf: "Epigenetic regulation of X-linked cancer/germline antigen genes by DNMT1 and DNMT3b." in: Oncogene, Vol. 25, Issue 52, pp. 6975-85, 2006 (PubMed). (Sample species: Human).

Robert, Morin, Beaulieu, Gauthier, Chute, Barsalou, MacLeod: "DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells." in: Nature genetics, Vol. 33, Issue 1, pp. 61-5, 2003 (PubMed). (Sample species: Human). Further details: Western Blotting

Bachman, Park, Rhee, Rajagopalan, Herman, Baylin, Kinzler, Vogelstein: "Histone modifications and silencing prior to DNA methylation of a tumor suppressor gene." in: Cancer cell, Vol. 3, Issue 1, pp. 89-95, 2003 (PubMed).

Yang, Seto: "Collaborative spirit of histone deacetylases in regulating chromatin structure and gene expression." in: Current opinion in genetics & development, Vol. 13, Issue 2, pp. 143-53, 2003 (PubMed).