Add to Basket
|+1 404 474 4654|
|+1 888 205 9894 (TF)|
DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) antibody
Alternatives Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|9 references available|
|Quantity||0.1 ml (Variants)|
|Price||Product not available in this region.|
|Immunogen||This antibody was raised against the first 10 kDa of the N-terminus of the DNMT1protein.|
|Description||Inactivation of tumor suppressor genes is a key component of the development of allcommon forms of human cancers. Aberrant methylation may contribute to many of thechanges, both physiological and pathological, associated with aging, including tumordevelopment. Hypermethylation of CpG island-containing promoters is associated withthe inactivation of genes important to tumor angiogenesis and progression. DNAmethytransferase (DNMT1) is necessary for the maintenance of the methylationprocess. Alternate Names: anti-DNMT-1 antibody, anti-DNMT 1 antibody, anti-DNMT antibody, anti-DNA9cytosine-5-)-methylatransferase 1 antibody, anti-MCMT antibody, anti-DNA MTaseMmu1 antibody, anti-DNA methyltransferase M.HsaI antibody.|
|Specificity||This antibody is specific for DNMT1. Species Reactivity: ABIN151732 recognizes human. Other species have not been tested.|
|Synonyms||AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET1, MET2, methyltransferase 1, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI|
|Application Notes||Suggested working dilutions: immunoprecipitation 1:1000,Western Blot 1:5000. Positive Controls: T24 cell lysates.|
|Buffer||Preservative: No preservative.|
|Storage||Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.|
|Restrictions||For Research Use only|
|Western blot analysis using ABIN151732 to detect DNMT1 levels in T24 cells. Lane 1:50 ugs of total protein lysate from untreated T24 cells. Lane 2: 50 ugs of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days.|
Rhee, Bachman, Park et al.: "DNMT1 and DNMT3b cooperate to silence genes in human cancer cells." in: Nature, Vol. 416, Issue 6880, pp. 552-6, 2002 (PubMed).
Suzuki, Gabrielson, Chen et al.: "A genomic screen for genes upregulated by demethylation and histone deacetylase inhibition in human colorectal cancer." in: Nature genetics, Vol. 31, Issue 2, pp. 141-9, 2002 (PubMed).
Beaulieu, Morin, Chute et al.: "An essential role for DNA methyltransferase DNMT3B in cancer cell survival." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28176-81, 2002 (PubMed).
Neumeister, Albanese, Balent et al.: "Senescence and epigenetic dysregulation in cancer." in: The international journal of biochemistry & cell biology, Vol. 34, Issue 11, pp. 1475-90, 2002 (PubMed).
Robert, Morin, Beaulieu et al.: "DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells." in: Nature genetics, Vol. 33, Issue 1, pp. 61-5, 2003 (PubMed).
Bachman, Park, Rhee et al.: "Histone modifications and silencing prior to DNA methylation of a tumor suppressor gene." in: Cancer cell, Vol. 3, Issue 1, pp. 89-95, 2003 (PubMed).
Yang, Seto: "Collaborative spirit of histone deacetylases in regulating chromatin structure and gene expression." in: Current opinion in genetics & development, Vol. 13, Issue 2, pp. 143-53, 2003 (PubMed).
Estueve, Chin, Smallwood et al.: "Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication." in: Genes & development, Vol. 20, Issue 22, pp. 3089-103, 2006 (PubMed).
Morey Kinney, Smiraglia, James et al.: "Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate model." in: Molecular cancer research : MCR, Vol. 6, Issue 8, pp. 1365-74, 2008 (PubMed).