DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) antibody

Antigen: DNA (Cytosine-5)-Methyltransferase 1 (DNMT1)

Synonyms: AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET1, MET2, methyltransferase 1, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN151732

Additional Information

Alternative name: DNMT1

Gene ID: 1786

Immunogen: This antibody was raised against the first 10 kDa of the N-terminus of the DNMT1protein.

Cross-Reactivity: Human

Format: Serum

Description: Inactivation of tumor suppressor genes is a key component of the development of allcommon forms of human cancers. Aberrant methylation may contribute to many of thechanges, both physiological and pathological, associated with aging, including tumordevelopment. Hypermethylation of CpG island-containing promoters is associated withthe inactivation of genes important to tumor angiogenesis and progression. DNAmethytransferase (DNMT1) is necessary for the maintenance of the methylationprocess. Alternate Names: anti-DNMT-1 antibody, anti-DNMT 1 antibody, anti-DNMT antibody, anti-DNA9cytosine-5-)-methylatransferase 1 antibody, anti-MCMT antibody, anti-DNA MTaseMmu1 antibody, anti-DNA methyltransferase M.HsaI antibody.

Specificity: This antibody is specific for DNMT1. Species Reactivity: ABIN151732 recognizes human. Other species have not been tested.

Synonyms: AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET1, MET2, methyltransferase 1, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI

Application Details

Application Notes: Suggested working dilutions: immunoprecipitation 1:1000,Western Blot 1:5000. Positive Controls: T24 cell lysates.

Purification: Serum

Buffer: Preservative: No preservative.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: DNA/RNA

Restrictions: For Research Use only

Images

Western blot analysis using ABIN151732 to detect DNMT1 levels in T24 cells. Lane 1:50 ugs of total protein lysate from untreated T24 cells. Lane 2: 50 ugs of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days.

Publications

Rhee, Bachman, Park et al.: "DNMT1 and DNMT3b cooperate to silence genes in human cancer cells." in: Nature, Vol. 416, Issue 6880, pp. 552-6, 2002 (PubMed).

Suzuki, Gabrielson, Chen et al.: "A genomic screen for genes upregulated by demethylation and histone deacetylase inhibition in human colorectal cancer." in: Nature genetics, Vol. 31, Issue 2, pp. 141-9, 2002 (PubMed).

Beaulieu, Morin, Chute et al.: "An essential role for DNA methyltransferase DNMT3B in cancer cell survival." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28176-81, 2002 (PubMed).

Neumeister, Albanese, Balent et al.: "Senescence and epigenetic dysregulation in cancer." in: The international journal of biochemistry & cell biology, Vol. 34, Issue 11, pp. 1475-90, 2002 (PubMed).

Robert, Morin, Beaulieu et al.: "DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells." in: Nature genetics, Vol. 33, Issue 1, pp. 61-5, 2003 (PubMed).

Bachman, Park, Rhee et al.: "Histone modifications and silencing prior to DNA methylation of a tumor suppressor gene." in: Cancer cell, Vol. 3, Issue 1, pp. 89-95, 2003 (PubMed).

Yang, Seto: "Collaborative spirit of histone deacetylases in regulating chromatin structure and gene expression." in: Current opinion in genetics & development, Vol. 13, Issue 2, pp. 143-53, 2003 (PubMed).

Estueve, Chin, Smallwood et al.: "Direct interaction between DNMT1 and G9a coordinates DNA and histone methylation during replication." in: Genes & development, Vol. 20, Issue 22, pp. 3089-103, 2006 (PubMed).

Morey Kinney, Smiraglia, James et al.: "Stage-specific alterations of DNA methyltransferase expression, DNA hypermethylation, and DNA hypomethylation during prostate cancer progression in the transgenic adenocarcinoma of mouse prostate model." in: Molecular cancer research : MCR, Vol. 6, Issue 8, pp. 1365-74, 2008 (PubMed).