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DNMT3A antibody (DNA (Cytosine-5-)-Methyltransferase 3 alpha)

Details for Product anti-DNMT3A Antibody No. ABIN151733, Supplier: Log in to see
Antigen
  • DNMT3A
  • DNMT3A2
  • M.HsaIIIA
  • MmuIIIA
Reactivity
Human, Mouse (Murine)
246
95
37
5
4
4
4
3
2
1
1
1
1
1
1
1
Host
Rabbit
195
53
2
2
1
Clonality
Polyclonal
Conjugate
This DNMT3A antibody is un-conjugated
14
13
11
10
8
8
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
216
98
60
57
45
33
12
11
6
6
4
2
1
Supplier
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Immunogen Amino acids 10-118 of human Dnmt3a [UniProt# Q9Y6K1]
Purification Unpurified
Alternative Name DNMT3A (DNMT3A Antibody Abstract)
Background Gene Symbol: DNMT3A
Molecular Weight Theoretical MW: 120 kDa
Gene ID 1788
UniProt Q9Y6K1
Application Notes Western Blot 1:1000, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:200
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-DNMT3A antibody (DNA (Cytosine-5-)-Methyltransferase 3 alpha) (ABIN151733) Western blot analysis using ABIN151733 to detect overexpressed DNMT3A level in HCT-11...
Immunohistochemistry (IHC) image for anti-DNMT3A antibody (DNA (Cytosine-5-)-Methyltransferase 3 alpha) (ABIN151733) Immunohistochemistry: Dnmt3a Antibody [ABIN151733] - Dnmt3a antibody was tested in hu...
Immunofluorescence (IF) image for anti-DNMT3A antibody (DNA (Cytosine-5-)-Methyltransferase 3 alpha) (ABIN151733) Immunocytochemistry/Immunofluorescence: Dnmt3a Antibody [ABIN151733] - Dnmt3a antibod...
Product cited in: Selvakumar, Gjidoda, Hovde, Henry: "Regulation of human RNA polymerase III transcription by DNMT1 and DNMT3a DNA methyltransferases." in: The Journal of biological chemistry, Vol. 287, Issue 10, pp. 7039-50, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Al-Salihi, Yu, Burnett, Alexander, Samlowski, Fitzpatrick: "The depletion of DNA methyltransferase-1 and the epigenetic effects of 5-aza-2'deoxycytidine (decitabine) are differentially regulated by cell cycle progression." in: Epigenetics, Vol. 6, Issue 8, pp. 1021-8, 2011 (PubMed). (Sample species: Human).

Kaminskyy, Surova, Vaculova, Zhivotovsky: "Combined inhibition of DNA methyltransferase and histone deacetylase restores caspase-8 expression and sensitizes SCLC cells to TRAIL." in: Carcinogenesis, Vol. 32, Issue 10, pp. 1450-8, 2011 (PubMed).

Shikauchi, Saiura, Kubo, Niwa, Yamamoto, Murase, Yoshikawa: "SALL3 interacts with DNMT3A and shows the ability to inhibit CpG island methylation in hepatocellular carcinoma." in: Molecular and cellular biology, Vol. 29, Issue 7, pp. 1944-58, 2009 (PubMed).

Fanelli, Caprodossi, Ricci-Vitiani, Porcellini, Tomassoni-Ardori, Amatori, Andreoni, Magnani, De Maria, Santoni, Minucci, Pelicci: "Loss of pericentromeric DNA methylation pattern in human glioblastoma is associated with altered DNA methyltransferases expression and involves the stem cell compartment." in: Oncogene, Vol. 27, Issue 3, pp. 358-65, 2008 (PubMed). (Sample species: Human).

Reynolds, Sigaroudinia, Zardo, Wilson, Benton, Miller, Hong, Fridlyand, Costello, Tlsty: "Tumor suppressor p16INK4A regulates polycomb-mediated DNA hypermethylation in human mammary epithelial cells." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24790-802, 2006 (PubMed).

La Salle, Trasler: "Dynamic expression of DNMT3a and DNMT3b isoforms during male germ cell development in the mouse." in: Developmental biology, Vol. 296, Issue 1, pp. 71-82, 2006 (PubMed). (Sample species: Mouse (Murine)).

Robert, Morin, Beaulieu, Gauthier, Chute, Barsalou, MacLeod: "DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells." in: Nature genetics, Vol. 33, Issue 1, pp. 61-5, 2003 (PubMed). (Sample species: Human). Further details: Western Blotting

Weisenberger, Velicescu, Preciado-Lopez, Gonzales, Tsai, Liang, Jones: "Identification and characterization of alternatively spliced variants of DNA methyltransferase 3a in mammalian cells." in: Gene, Vol. 298, Issue 1, pp. 91-9, 2002 (PubMed).

Beaulieu, Morin, Chute, Robert, Nguyen, MacLeod: "An essential role for DNA methyltransferase DNMT3B in cancer cell survival." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28176-81, 2002 (PubMed). (Sample species: Human). Further details: Western Blotting