Detection of Human NBS1 by Immunoprecipitation and Western Blot. Sample: Nuclear extract (50 mcg for WB, 1 mg for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-NBS1 (ABIN151745) used at 0.08 mcg/ml for WB and 1.25 mcg/mg lysate for IP. Detection: Chemiluminescence. (* band is believed to be a proteolytic fragment of NBS1).
Western Blot: NBS1 Antibody [ABIN151745] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NBS1 antibody (Lot #2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NBS1 was also immunoprecipitated by a prior batch (Lot #1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).