Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Antigen: Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)

Synonyms: HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a

Clonality: Monoclonal (HA111)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN151762

Additional Information

Alternative name: HIF1A / HIF1 alpha

Gene ID: 3091

UniProt: Q16665

Immunogen: Human HIF-1 alpha amino acids 329-530.

Cross-Reactivity: Human

Isotype: IgG2a

Clone: HA111

Description: Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF hydroxylases provide insight into hypoxic cell responses, which may be used to helpisolate therapeutic targets. Alternate Names: anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF1 alpha antibody, anti-ARNTinteracting proteinantibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody,anti-HIF-1alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxiainducible factor 1 alpha subunit basic helix antibody.

Specificity: This antibody is specific for HIF-1 alpha. Species Reactivity: ABIN151762 recognizes human. Other species have not been tested. Localization: Cytoplasmic in normoxia, nuclear translocation in response to hypoxia.

Application Details

Application Notes: Nuclear extracts should be used for Western analysis

Concentration: 1.1 mg/ml

Purification: Purified

Buffer: Preservative: 0.1% sodium azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Images

Figure 1 illustrates Westerm blot detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein using ABIN151762. (explants were subjected to 21 and 2% oxygen for 4 hours) using ABIN151428.

Publications

Gillespie, Whang, Ragel et al.: "Silencing of hypoxia inducible factor-1alpha by RNA interference attenuates human glioma cell growth in vivo." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 8, pp. 2441-8, 2007 (PubMed).

Lauzier, Robitaille, Chan et al.: "(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors." in: Molecular pharmacology, Vol. 74, Issue 1, pp. 282-8, 2008 (PubMed).