Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Details for Product No. ABIN151762
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Antigen
Synonyms HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hif1a, HIF1-alpha, HIF-1a, HIF1a, hif-1a
Reactivity
Human
(245), (101), (80), (56), (38), (34), (20), (19), (17), (16), (12), (2), (2), (2), (1), (1)
Host
Mouse
(174), (112), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(27), (14), (12), (12), (12), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(231), (79), (79), (67), (58), (50), (36), (28), (15), (11), (10), (9), (7), (4), (1), (1)
Pubmed 2 references available
Catalog no. ABIN151762
Quantity 0.1 mL
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Immunogen Human HIF-1 alpha amino acids 329-530.
Clone HA111
Isotype IgG2a
Specificity This antibody is specific for HIF-1 alpha. Species Reactivity: ABIN151762 recognizes human. Other species have not been tested. Localization: Cytoplasmic in normoxia, nuclear translocation in response to hypoxia.
Cross-Reactivity Human
Purification Purified
Alternative Name HIF1A / HIF1 alpha
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF hydroxylases provide insight into hypoxic cell responses, which may be used to helpisolate therapeutic targets. Alternate Names: anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF1 alpha antibody, anti-ARNTinteracting proteinantibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody,anti-HIF-1alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxiainducible factor 1 alpha subunit basic helix antibody.
Gene ID 3091
UniProt Q16665
Application Notes Nuclear extracts should be used for Western analysis
Restrictions For Research Use only
Format Liquid
Concentration 1.1 mg/ml
Buffer Preservative: 0.1% sodium azide.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody Figure 1 illustrates Westerm blot detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein using ABIN151762. (explants were subjected to 21 and 2% oxygen for 4 hours) using ABIN151428.
General Gillespie, Whang, Ragel et al.: "Silencing of hypoxia inducible factor-1alpha by RNA interference attenuates human glioma cell growth in vivo." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 8, pp. 2441-8, 2007 (PubMed).

Lauzier, Robitaille, Chan et al.: "(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors." in: Molecular pharmacology, Vol. 74, Issue 1, pp. 282-8, 2008 (PubMed).

Hosts (174), (112), (2), (1)
Reactivities (245), (101), (80), (56), (38), (34), (20), (19), (17), (16), (12), (2), (2), (2), (1), (1)
Applications (231), (79), (79), (67), (58), (50), (36), (28), (15), (11), (10), (9), (7), (4), (1), (1)
Conjugates (27), (14), (12), (12), (12), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (36), (23), (15), (10), (8), (7), (7), (6), (6), (6), (5), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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