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Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Details for Product No. ABIN151762, Supplier: Log in to see
Antigen
  • AA959795
  • bHLHe78
  • hif-1a
  • HIF-1A
  • HIF-1alpha
  • HIF1
  • HIF1-alpha
  • HIF1-ALPHA
  • hif1a
  • HIF1alpha
  • MOP1
  • PASD8
Reactivity
Human
584
264
203
119
85
69
61
56
50
50
38
36
22
19
18
17
12
12
1
1
1
1
1
1
1
1
Host
Mouse
378
277
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
51
33
32
26
25
25
17
17
14
14
12
12
6
6
6
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
497
221
205
191
177
157
102
92
48
15
11
11
10
7
7
4
4
3
3
2
2
1
1
Supplier
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Immunogen Human HIF-1 alpha, corresponding to amino acids 329 - 530 [UniProt# Q16665].
Clone HA111
Isotype IgG2a
Purification Protein G purified
Alternative Name HIF-1 alpha (HIF1A Antibody Abstract)
Background Gene Symbol: HIF1A
Molecular Weight Theoretical MW: 93 kDa
Gene ID 3091
UniProt Q16665
Application Notes Western Blot 0.5 - 1.0 mg/mL, Immunohistochemistry 1:100, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:100
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-Paraffin Embedded Sections Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
1. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 degrees C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody (ABIN151762) Figure 1 illustrates Westerm blot detection of HIF-1 alpha (125-130 kDa) from human ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody (ABIN151762) Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (HA111) [ABIN151762] - HIF-1 anti...
Product cited in: Lee, Spata, Bayne, Buza, Durham, Allman, Vonderheide, Simon: "Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia." in: Cancer discovery, Vol. 6, Issue 3, pp. 256-69, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Lauzier, Robitaille, Chan, Giaccia, Richard: "(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors." in: Molecular pharmacology, Vol. 74, Issue 1, pp. 282-8, 2008 (PubMed).

Rajakumar, Michael, Daftary, Jeyabalan, Gilmour, Conrad: "Proteasomal activity in placentas from women with preeclampsia and intrauterine growth restriction: implications for expression of HIF-alpha proteins." in: Placenta, Vol. 29, Issue 3, pp. 290-9, 2008 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Gillespie, Whang, Ragel, Flynn, Kelly, Jensen: "Silencing of hypoxia inducible factor-1alpha by RNA interference attenuates human glioma cell growth in vivo." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 8, pp. 2441-8, 2007 (PubMed).

Zheng, Ruas, Cao, Salomons, Cao, Poellinger, Pereira: "Cell-type-specific regulation of degradation of hypoxia-inducible factor 1 alpha: role of subcellular compartmentalization." in: Molecular and cellular biology, Vol. 26, Issue 12, pp. 4628-41, 2006 (PubMed). (Sample species: Rabbit). Further details: Western Blotting,Immunoprecipitation

Jensen, Ragel, Whang, Gillespie: "Inhibition of hypoxia inducible factor-1alpha (HIF-1alpha) decreases vascular endothelial growth factor (VEGF) secretion and tumor growth in malignant gliomas." in: Journal of neuro-oncology, Vol. 78, Issue 3, pp. 233-47, 2006 (PubMed).