Egl Nine Homolog 3 (C. Elegans) (EGLN3) antibody

Details for Product No. ABIN151769
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Synonyms HIFP4H3, HIFPH3, PHD3, 2610021G09Rik, AI505553, AI648162, Hif-p4h-3, Phd3, SM-20, phd3, PHD-3, zgc:77019, wu:fj78a08, EGLN3
(51), (23), (23), (17), (14), (13), (6), (1), (1)
(36), (14), (5)
(5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(43), (12), (10), (10), (5), (2), (1), (1), (1)
Pubmed 7 references available
Catalog no. ABIN151769
Quantity 0.1 mL
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Immunogen A synthetic peptide made to the C-terminus of the human protein sequence of HIF prolylhydroxylase 3. [LocusLink ID 112399].
Specificity This antibody is specific for HIF prolyl hydroxylase 3. Species Reactivity: Human and Mouse. Reactivity is likely in Rat due to 26/28 residue homology betweenthe Rat protein and the peptide used as the immunogen for this antibody.
Cross-Reactivity Human, Mouse (Murine)
Purification Aff - Purified
Alternative Name EGLN3 / PHD3
Background HIF prolyl hydroxylase 3 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis.HIF prolyl hydroxylase 1, specifically, catalyzes the posttranslational formation of4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(564) andHIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation viathe von Hippel-Lindau ubiquitylation complex. It may also play a role in cell growthregulation in muscle cells and in apoptosis in neuronal tissues, promoting cell deaththrough a caspase-dependent mechanism. Alternate Names: anti-EGL9 homolog of C. elegans 3 antibody, anti-EGLN3 antibody, anti-Factorresponsive smooth muscle protein antibody, anti-PHD3 antibody, anti-HIF ProlylHydroxylase 3 antibody, anti-HIFP4H3 antibody, anti-HIFPH3 antibody, anti-P4H3antibody, anti-Prolyl Hydroxylase Domain Containing Protein 3 antibody, anti-SM20antibody.
Gene ID 112399, 112407
Application Notes Suggested working dilutions: Western Blot 1:500 to 1:2,500
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/ml
Buffer Tris-citrate/phosphate [pH 7-8]. Preservative: 0.09% Sodium Azide
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-Egl Nine Homolog 3 (C. Elegans) (EGLN3) antibody Western Blot analysis of human PHD3, using ABIN151769. Samples: HeLa whole cell lysates and MEFs.
General Bruick, McKnight: "A conserved family of prolyl-4-hydroxylases that modify HIF." in: Science (New York, N.Y.), Vol. 294, Issue 5545, pp. 1337-40, 2001 (PubMed).

Ivan, Haberberger, Gervasi et al.: "Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 21, pp. 13459-64, 2002 (PubMed).

Ietta, Wu, Winter et al.: "Dynamic HIF1A regulation during human placental development." in: Biology of reproduction, Vol. 75, Issue 1, pp. 112-21, 2006 (PubMed).

Takeda, Aguila, Parikh et al.: "Regulation of adult erythropoiesis by prolyl hydroxylase domain proteins." in: Blood, Vol. 111, Issue 6, pp. 3229-35, 2008 (PubMed).

Minamishima, Moslehi, Bardeesy et al.: "Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure." in: Blood, Vol. 111, Issue 6, pp. 3236-44, 2008 (PubMed).

Mikhaylova, Ignacak, Barankiewicz et al.: "The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress." in: Molecular and cellular biology, Vol. 28, Issue 8, pp. 2701-17, 2008 (PubMed).

Rodríguez-Jiménez, Moreno-Manzano, Lucas-Dominguez et al.: "Hypoxia causes downregulation of mismatch repair system and genomic instability in stem cells." in: Stem cells (Dayton, Ohio), Vol. 26, Issue 8, pp. 2052-62, 2008 (PubMed).

Hosts (36), (14), (5)
Reactivities (51), (23), (23), (17), (14), (13), (6), (1), (1)
Applications (43), (12), (10), (10), (5), (2), (1), (1), (1)
Conjugates (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (5), (3), (2), (1), (1), (1), (1)
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