EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (C-Term)

Details for Product anti-EGLN3 Antibody No. ABIN151769, Supplier: Log in to see
Antigen
  • zgc:77019
  • wu:fj78a08
  • EGLN3
  • phd3
  • PHD-3
  • PHD3
  • SM-20
  • 2610021G09Rik
  • AI505553
  • AI648162
  • Hif-p4h-3
  • Phd3
  • HIFP4H3
  • HIFPH3
  • egl nine homolog 3 (C. elegans)
  • egl nine homolog 3
  • EGL nine homolog 3 (C. elegans)
  • egl-9 family hypoxia-inducible factor 3
  • egln3
  • LOC100354210
  • EGLN3
  • LOC100219039
  • Egln3
Alternatives
anti-Primate EGLN3 antibody for Chromatin Immunoprecipitation
Epitope
C-Term
31
12
8
8
7
6
3
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Primate
124
51
46
23
8
7
7
5
4
3
2
2
2
2
1
1
Host
Rabbit
86
30
16
Clonality
Polyclonal
Conjugate
This EGLN3 antibody is un-conjugated
11
11
8
7
7
7
3
3
3
3
3
3
3
3
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
114
47
44
42
26
22
3
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen A synthetic peptide made to the C-terminus of humanPHD3/HIF Prolyl Hydroxylase 3. [LocusLink ID 112399]
Purification Immunogen affinity purified
Alternative Name EGLN3/PHD3 (EGLN3 Antibody Abstract)
Background Gene Symbol: EGLN3
Molecular Weight Theoretical MW: 27 kDa
Gene ID 112399
Pathways Positive Regulation of Endopeptidase Activity
Application Notes Western Blot 1:500-1:2000, Simple Western 1:1000, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-ParaffinThis PHD3/HIF Prolyl Hydroxylase 3 antibody is useful for Immunoprecipitation and Western blot, where a band is seen at approx. 27 kDa. This antibody has been tested against HeLa and MEF cell lysates. Immunoprecipitation was reported in PMID: 21575608. Chromatin Immunoprecipitation was reported in scientific literature.Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25161887). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 20801873) In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for HIF Prolyl Hydroxylase 3 Antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 35 µg of sample lysate per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (C-Term) (ABIN151769) Western Blot analysis of human PHD3, using ABIN151769. Samples: HeLa whole cell lysat...
Simple Western (SimWes) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (C-Term) (ABIN151769) Simple Western: EGLN3/PHD3 Antibody [ABIN151769] - Simple Western lane view shows a s...
Western Blotting (WB) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (C-Term) (ABIN151769) Western Blot: EGLN3/PHD3 Antibody [ABIN151769] - Total protein from Mouse MEF, Human ...
Western Blotting (WB) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (C-Term) (ABIN151769) Western Blot: EGLN3/PHD3 Antibody [ABIN151769] - Whole cell protein from MEF cells wa...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (C-Term) (ABIN151769) Immunohistochemistry-Paraffin: EGLN3/PHD3 Antibody [ABIN151769] - Egl nine homolog 3 ...
Immunofluorescence (IF) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (C-Term) (ABIN151769) Immunocytochemistry/Immunofluorescence: EGLN3/PHD3 Antibody [ABIN151769] - analysis o...
Immunofluorescence (IF) image for anti-EGLN3 antibody (Egl Nine Homolog 3 (C. Elegans)) (C-Term) (ABIN151769) Immunocytochemistry/Immunofluorescence: EGLN3/PHD3 Antibody [ABIN151769] - HeLa cells...
Product cited in: Gibier, Hémon, Fanchon, Gaudelot, Pottier, Ringot, Van Seuningen, Glowacki, Cauffiez, Blum, Copin, Perrais, Gnemmi: "Dual role of MUC1 mucin in kidney ischemia-reperfusion injury: Nephroprotector in early phase, but pro-fibrotic in late phase." in: Biochimica et biophysica acta, Vol. 1863, Issue 6, pp. 1336-1349, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Shin, Nunomiya, Kitajima, Dan, Miyata, Nagatomi: "Prolyl hydroxylase domain 2 deficiency promotes skeletal muscle fiber-type transition via a calcineurin/NFATc1-dependent pathway." in: Skeletal muscle, Vol. 6, pp. 5, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Heir, Srikumar, Bikopoulos, Bunda, Poon, Lee, Raught, Ohh: "Oxygen-dependent Regulation of Erythropoietin Receptor Turnover and Signaling." in: The Journal of biological chemistry, Vol. 291, Issue 14, pp. 7357-72, 2016 (PubMed).

Tan, Prosser, Dunn, Vanags, Ridiandries, Tsatralis, Leece, Clayton, Yuen, Robertson, Lam, Celermajer, Ng, Bursill: "High-Density Lipoproteins Rescue Diabetes-Impaired Angiogenesis via Scavenger Receptor Class B Type I." in: Diabetes, Vol. 65, Issue 10, pp. 3091-103, 2016 (PubMed).

Zieseniss, Hesse, Jatho, Krull, Hölscher, Vogel, Katschinski: "Cardiomyocyte-Specific Transgenic Expression of Prolyl-4-Hydroxylase Domain 3 Impairs the Myocardial Response to Ischemia." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 3, pp. 843-51, 2015 (PubMed).

Kim, Kwak, Cha, Jeong, Rhee, Cheon: "The role of prolyl hydroxylase domain protein (PHD) during rosiglitazone-induced adipocyte differentiation." in: The Journal of biological chemistry, Vol. 289, Issue 5, pp. 2755-64, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Reischl, Li, Walkinshaw, Flippin, Marti, Kunze: "Inhibition of HIF prolyl-4-hydroxylases by FG-4497 reduces brain tissue injury and edema formation during ischemic stroke." in: PLoS ONE, Vol. 9, Issue 1, pp. e84767, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Kim, Neinast, Frank, Sun, Park, Zehr, Vishvanath, Morselli, Amelotte, Palmer, Gupta, Scherer, Clegg: "ERα upregulates Phd3 to ameliorate HIF-1 induced fibrosis and inflammation in adipose tissue." in: Molecular metabolism, Vol. 3, Issue 6, pp. 642-51, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Garvalov, Foss, Henze, Bethani, Gräf-Höchst, Singh, Filatova, Dopeso, Seidel, Damm, Acker-Palmer, Acker: "PHD3 regulates EGFR internalization and signalling in tumours." in: Nature communications, Vol. 5, pp. 5577, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Tan, Prosser, Vanags, Monger, Ng, Bursill: "High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1α." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 1, pp. 206-17, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Swain, Wottawa, Hillemann, Beneke, Odagiri, Terada, Endo, Oike, Farhat, Katschinski: "Prolyl-4-hydroxylase domain 3 (PHD3) is a critical terminator for cell survival of macrophages under stress conditions." in: Journal of leukocyte biology, Vol. 96, Issue 3, pp. 365-75, 2014 (PubMed). (Sample species: Mouse (Murine)).

Lindholm, Fischer, Poellinger, Johnson, Gustafsson, Sundberg, Rundqvist: "Negative regulation of HIF in skeletal muscle of elite endurance athletes: a tentative mechanism promoting oxidative metabolism." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 307, Issue 3, pp. R248-55, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Henze, Garvalov, Seidel, Cuesta, Ritter, Filatova, Foss, Dopeso, Essmann, Maxwell, Reifenberger, Carmeliet, Acker-Palmer, Acker: "Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR." in: Nature communications, Vol. 5, pp. 5582, 2014 (PubMed).

Fu, Taubman: "EGLN3 inhibition of NF-κB is mediated by prolyl hydroxylase-independent inhibition of IκB kinase γ ubiquitination." in: Molecular and cellular biology, Vol. 33, Issue 15, pp. 3050-61, 2013 (PubMed). Further details: Western Blotting

Galán-Cobo, Sánchez-Silva, Serna, Abreu-Rodríguez, Muñoz-Cabello, Echevarría: "Cellular overexpression of aquaporins slows down the natural HIF-2α degradation during prolonged hypoxia." in: Gene, Vol. 522, Issue 1, pp. 18-26, 2013 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Michailidou, Turban, Miller, Zou, Schrader, Ratcliffe, Hadoke, Walker, Iredale, Morton, Seckl: "Increased angiogenesis protects against adipose hypoxia and fibrosis in metabolic disease-resistant 11β-hydroxysteroid dehydrogenase type 1 (HSD1)-deficient mice." in: The Journal of biological chemistry, Vol. 287, Issue 6, pp. 4188-97, 2012 (PubMed).

Kunze, Zhou, Veltkamp, Wielockx, Breier, Marti: "Neuron-specific prolyl-4-hydroxylase domain 2 knockout reduces brain injury after transient cerebral ischemia." in: Stroke, Vol. 43, Issue 10, pp. 2748-56, 2012 (PubMed). (Sample species: Mouse (Murine)).

Yan, Jiao, Zhang, Lv, Xue, Fan, Wu, Fang: "Prolyl hydroxylase domain protein 3 targets Pax2 for destruction." in: Biochemical and biophysical research communications, Vol. 409, Issue 2, pp. 315-20, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Rabie, Kunze, Marti: "Impaired hypoxic response in senescent mouse brain." in: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Vol. 29, Issue 6, pp. 655-61, 2011 (PubMed).

Luo, Hu, Chang, Zhong, Knabel, OMeally, Cole, Pandey, Semenza: "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1." in: Cell, Vol. 145, Issue 5, pp. 732-44, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation