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TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400)

Details for Product anti-TP53BP1 Antibody No. ABIN151770, Supplier: Log in to see
Antigen
  • TP53BP1
  • MGC160112
  • 53BP1
  • Tp53bp1
  • m53BP1
  • p202
  • Trp53bp1
  • tumor protein p53 binding protein 1
  • tumor protein p53 binding protein 1 (predicted), 5 prime
  • transformation related protein 53 binding protein 1
  • TP53BP1
  • tp53bp1
  • Trp53bp1
  • Tp53bp1
Alternatives
anti-Human TP53BP1 antibody for Western Blotting
Epitope
AA 350-400
44
27
17
16
15
10
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6
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Fish, Goat, Human, Mouse (Murine), Primate, Rat (Rattus)
236
152
69
18
18
12
12
4
4
4
3
2
2
1
Host
Rabbit
204
28
3
1
Clonality
Polyclonal
Conjugate
This TP53BP1 antibody is un-conjugated
10
10
8
8
7
7
7
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
179
90
87
80
79
52
35
27
24
11
7
6
5
2
2
1
Options
Supplier
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Immunogen The epitope recognized by this antibody maps to a region between residues 350 and 400 of human 53BP1 [NP_005648.1].
Isotype IgG
Purification Immunogen affinity purified
Alternative Name 53BP1 (TP53BP1 Antibody Abstract)
Background Gene Symbol: TP53BP1
Molecular Weight Theoretical MW: 214 kDa
Gene ID 7158
NCBI Accession NP_005648
Pathways DNA Damage Repair
Application Notes Western Blot 1:5000-1:25000, Chromatin Immunoprecipitation, Flow Cytometry 1.5 μg/mL, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:1000-1:5000, Immunoprecipitation, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-FrozenThis 53BP1 antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry and Flow Cytometry. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunohistochemistry-Paraffin and Immunohistochemistry were reported in scientific literature. Frozen section data from customer review. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24591601) Use in Immunoprecipitation reported in scientific literature (PMID 25645366).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry Protocol Specific for NB100-304: 53BP1 Antibody Materials1) 1 Phosphate buffered saline (pH 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/L2) Citrate buffer, 0.01 M, pH6.0, Sodium Citrate 3g, Citric acid 0.4g3) 3 % Hydrogen peroxide4) Primary antibody5) Blocking serum (normal serum)6) Biotinylated secondary antibody7) DAB staining kitMethods
1. Dewax and hydration of slides using xylene and EtOH:Dry slides for 20 min in a 60 C ovenAdd Xylene, 2 x 10 min100 %, 95 %, 80 %, and 70 % EtOH, 5 min each EtOH concentration Rinse in PBS, 5'2 Antigen retrieval method (only for paraffin slides)1a. High-pressure antigen retrieval procedure (recommended method)Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 mL of Citrate buffer (0.01M, pH6.
0) within a pressure cookerOnce steam is produced, and ONLY when steam is visible, from the pressure cooker (usually 15-20 min), the required high-pressure will have been reached, and slides will be incubated for 2 min.Turn off heat, and allow buffer and slides to cool to room temperatureSlides are then rinsed in PBS for 5 minutes
. Add 3 % hydrogen peroxide solution, 10'at RT, then PBS, 3X5'
. Normal blocking serum, 20'at RT
. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C
. Rinse with PBS, 3 X 5' each rinse
. Add Biotin-conjugated second antibody, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Add Streptavidin-Peroxidase, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Staining with DAB solution, 2-5'under microscope
. Stop the reaction by washing in tap water
. Counterstain in Haematoxylin for 3-5 minutes
. 75 %, 80 %, 95 % and 100 % ethanol, 5x2', xylene 2 x 10'
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage -20 °C
Storage Comment Store at -20 °C.
Supplier Images
Immunofluorescence (IF) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 350-400) antibody
Immunofluorescence (IF) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 350-400) antibody (Image 2)
Western Blotting (WB) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 350-400) antibody (Image 3)
Flow Cytometry (FACS) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Flow Cytometry: 53BP1 Antibody [ABIN151770] - 1 million Jurkat cells were fixed, perm...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Immunohistochemistry-Paraffin: 53BP1 Antibody [ABIN151770] - Human breast tumors stai...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Immunohistochemistry-Paraffin: 53BP1 Antibody [ABIN151770] - IHC staining of 53BP1 in...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Immunohistochemistry-Paraffin: 53BP1 Antibody [ABIN151770] - Detection of Human and M...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Immunohistochemistry-Frozen: 53BP1 Antibody [ABIN151770] - Irradiated cochlear spiral...
Immunofluorescence (IF) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [ABIN151770] - 53BP1 antibody ...
Product cited in: Ortega-Martínez: "Dexamethasone acts as a radiosensitizer in three astrocytoma cell lines via oxidative stress." in: Redox biology, Vol. 5, pp. 388-97, 2016 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Durdik, Kosik, Gursky, Vokalova, Markova, Belyaev: "Imaging flow cytometry as a sensitive tool to detect low-dose-induced DNA damage by analyzing 53BP1 and γH2AX foci in human lymphocytes." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 87, Issue 12, pp. 1070-8, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Flow Cytometry,Immunofluorescence

Kubben, Brimacombe, Donegan, Li, Misteli: "A high-content imaging-based screening pipeline for the systematic identification of anti-progeroid compounds." in: Methods (San Diego, Calif.), Vol. 96, pp. 46-58, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Chaudhary, Marshall, Currell, Kacperek, Schettino, Prise: "Variations in the Processing of DNA Double-Strand Breaks Along 60-MeV Therapeutic Proton Beams." in: International journal of radiation oncology, biology, physics, Vol. 95, Issue 1, pp. 86-94, 2016 (PubMed).

Marková, Somsedíková, Vasilyev, Pobijaková, Lacková, Lukačko, Belyaev: "DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy." in: International journal of radiation biology, Vol. 91, Issue 12, pp. 934-45, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Chatalic, Konijnenberg, Nonnekens, de Blois, Hoeben, de Ridder, Brunel, Fehrentz, Martinez, van Gent, Nock, Maina, van Weerden, de Jong: "In Vivo Stabilization of a Gastrin-Releasing Peptide Receptor Antagonist Enhances PET Imaging and Radionuclide Therapy of Prostate Cancer in Preclinical Studies." in: Theranostics, Vol. 6, Issue 1, pp. 104-17, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Zhang, Liu, Chen, Yang, Wang, Liu, Deng, Qin, Correia, Lee, Kim, Sparks, Nair, Evans, Kalari, Zhang, Wang, You, Kaufmann, Lou, Pei: "A cell cycle-dependent BRCA1-UHRF1 cascade regulates DNA double-strand break repair pathway choice." in: Nature communications, Vol. 7, pp. 10201, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Lescale, Abramowski, Bedora-Faure, Murigneux, Vera, Roth, Revy, de Villartay, Deriano: "RAG2 and XLF/Cernunnos interplay reveals a novel role for the RAG complex in DNA repair." in: Nature communications, Vol. 7, pp. 10529, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Pauty, Côté, Rodrigue, Velic, Masson, Fortin: "Investigation of the DNA damage response to SFOM-0046, a new small-molecule drug inducing DNA double-strand breaks." in: Scientific reports, Vol. 6, pp. 23302, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Kreienkamp, Croke, Neumann, Bedia-Diaz, Graziano, Dusso, Dorsett, Carlberg, Gonzalo: "Vitamin D receptor signaling improves Hutchinson-Gilford progeria syndrome cellular phenotypes." in: Oncotarget, Vol. 7, Issue 21, pp. 30018-31, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Ho, Guilbaud, Blow, Sale, Watson: "The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability." in: Cell reports, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Stankovic-Valentin, Drzewicka, König, Schiebel, Melchior: "Redox regulation of SUMO enzymes is required for ATM activity and survival in oxidative stress." in: The EMBO journal, Vol. 35, Issue 12, pp. 1312-29, 2016 (PubMed).

Jullien, Vignard, Fedor, Béry, Olichon, Crozatier, Erard, Cassard, Ducommun, Salles, Mirey: "Chromatibody, a novel non-invasive molecular tool to explore and manipulate chromatin in living cells." in: Journal of cell science, Vol. 129, Issue 13, pp. 2673-83, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Parekh, Choudhuri, Weyemi, Martin, Bonner, Redon: "Evaluation of surrogate tissues as indicators of drug activity in a melanoma skin model." in: Cancer medicine, Vol. 5, Issue 8, pp. 1731-41, 2016 (PubMed). Further details: Immunohistochemistry

Fong, Mazo, Das, Goodman, Kim, ORourke, Izquierdo, Tsou: "53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis." in: eLife, Vol. 5, 2016 (PubMed).

Mao, Liu, Zhang, Zhang, Liu, Gao, Rong, Zhao: "Homologous recombination-dependent repair of telomeric DSBs in proliferating human cells." in: Nature communications, Vol. 7, pp. 12154, 2016 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Lambrus, Daggubati, Uetake, Scott, Clutario, Sluder, Holland: "A USP28-53BP1-p53-p21 signaling axis arrests growth after centrosome loss or prolonged mitosis." in: The Journal of cell biology, Vol. 214, Issue 2, pp. 143-53, 2016 (PubMed). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Butterworth, Nicol, Ghita, Rosa, Chaudhary, McGarry, McCarthy, Jimenez-Sanchez, Bazzi, Roux, Tillement, Coulter, Prise: "Preclinical evaluation of gold-DTDTPA nanoparticles as theranostic agents in prostate cancer radiotherapy." in: Nanomedicine (London, England), Vol. 11, Issue 16, pp. 2035-47, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Chua, Lee, Chiang, Chen, Lu, Chen, Yang, Lin, Chen, Kao, Chen, Chang, Yip: "The cardioprotective effect of melatonin and exendin-4 treatment in a rat model of cardiorenal syndrome." in: Journal of pineal research, Vol. 61, Issue 4, pp. 438-456, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Patel, Suram, Mirani, Bischof, Herbig: "Derepression of hTERT gene expression promotes escape from oncogene-induced cellular senescence." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 34, pp. E5024-33, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence