TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400)

Details for Product anti-TP53BP1 Antibody No. ABIN151770, Supplier: Log in to see
Antigen
  • TP53BP1
  • MGC160112
  • 53BP1
  • Tp53bp1
  • m53BP1
  • p202
  • Trp53bp1
  • tumor protein p53 binding protein 1
  • tumor protein p53 binding protein 1 (predicted), 5 prime
  • transformation related protein 53 binding protein 1
  • TP53BP1
  • tp53bp1
  • Trp53bp1
  • Tp53bp1
Alternatives
anti-Rat (Rattus) TP53BP1 antibody for Chromatin Immunoprecipitation
Epitope
AA 350-400
48
27
17
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2
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2
1
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1
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1
Reactivity
Fish, Goat, Human, Mouse (Murine), Primate, Rat (Rattus)
238
153
67
18
18
12
12
4
4
4
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2
2
1
Host
Rabbit
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28
3
1
Clonality
Polyclonal
Conjugate
This TP53BP1 antibody is un-conjugated
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10
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5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
179
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81
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49
39
27
24
11
7
6
5
2
2
1
Options
Supplier
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Immunogen The epitope recognized by this antibody maps to a region between residues 350 and 400 of human 53BP1 [NP_005648.1].
Isotype IgG
Purification Immunogen affinity purified
Alternative Name 53BP1 (TP53BP1 Antibody Abstract)
Background Gene Symbol: TP53BP1
Molecular Weight Theoretical MW: 214 kDa
Gene ID 7158
NCBI Accession NP_005648
Pathways DNA Damage Repair
Application Notes Western Blot 1:5000-1:25000, Chromatin Immunoprecipitation, Flow Cytometry 1.5 μg/mL, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:1000-1:5000, Immunoprecipitation, Immunohistochemistry-Paraffin 1:1000-1:5000, Immunohistochemistry-FrozenThis 53BP1 antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry and Flow Cytometry. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunohistochemistry-Paraffin and Immunohistochemistry were reported in scientific literature. Frozen section data from customer review. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24591601) Use in Immunoprecipitation reported in scientific literature (PMID 25645366).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry Protocol Specific for NB100-304: 53BP1 Antibody Materials1) 1 Phosphate buffered saline (pH 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/L2) Citrate buffer, 0.01 M, pH6.0, Sodium Citrate 3g, Citric acid 0.4g3) 3 % Hydrogen peroxide4) Primary antibody5) Blocking serum (normal serum)6) Biotinylated secondary antibody7) DAB staining kitMethods
1. Dewax and hydration of slides using xylene and EtOH:Dry slides for 20 min in a 60 C ovenAdd Xylene, 2 x 10 min100 %, 95 %, 80 %, and 70 % EtOH, 5 min each EtOH concentration Rinse in PBS, 5'2 Antigen retrieval method (only for paraffin slides)1a. High-pressure antigen retrieval procedure (recommended method)Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 mL of Citrate buffer (0.01M, pH6.
0) within a pressure cookerOnce steam is produced, and ONLY when steam is visible, from the pressure cooker (usually 15-20 min), the required high-pressure will have been reached, and slides will be incubated for 2 min.Turn off heat, and allow buffer and slides to cool to room temperatureSlides are then rinsed in PBS for 5 minutes
. Add 3 % hydrogen peroxide solution, 10'at RT, then PBS, 3X5'
. Normal blocking serum, 20'at RT
. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C
. Rinse with PBS, 3 X 5' each rinse
. Add Biotin-conjugated second antibody, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Add Streptavidin-Peroxidase, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Staining with DAB solution, 2-5'under microscope
. Stop the reaction by washing in tap water
. Counterstain in Haematoxylin for 3-5 minutes
. 75 %, 80 %, 95 % and 100 % ethanol, 5x2', xylene 2 x 10'
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage -20 °C
Storage Comment Store at -20 °C.
Supplier Images
Immunofluorescence (IF) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 350-400) antibody
Immunofluorescence (IF) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 350-400) antibody (Image 2)
Western Blotting (WB) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 350-400) antibody (Image 3)
Flow Cytometry (FACS) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Flow Cytometry: 53BP1 Antibody [ABIN151770] - 1 million Jurkat cells were fixed, perm...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Immunohistochemistry-Paraffin: 53BP1 Antibody [ABIN151770] - Human breast tumors stai...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Immunohistochemistry-Paraffin: 53BP1 Antibody [ABIN151770] - IHC staining of 53BP1 in...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Immunohistochemistry-Paraffin: 53BP1 Antibody [ABIN151770] - Detection of Human and M...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Immunohistochemistry-Frozen: 53BP1 Antibody [ABIN151770] - Irradiated cochlear spiral...
Immunofluorescence (IF) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (AA 350-400) (ABIN151770) Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [ABIN151770] - 53BP1 antibody ...
Product cited in: Dixon, Platani, Makarov, Schirmer: "Microinjection of Antibodies Targeting the Lamin A/C Histone-Binding Site Blocks Mitotic Entry and Reveals Separate Chromatin Interactions with HP1, CenpB and PML." in: Cells, Vol. 6, Issue 2, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Feldman, Wuerffel, Achour, Wang, Carpenter, Kenter: "53BP1 Contributes to Igh Locus Chromatin Topology during Class Switch Recombination." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 6, pp. 2434-2444, 2017 (PubMed). (Sample species: Mouse (Murine)).

Rossiello, Aguado, Sepe, Iannelli, Nguyen, Pitchiaya, Carninci, dAdda di Fagagna: "DNA damage response inhibition at dysfunctional telomeres by modulation of telomeric DNA damage response RNAs." in: Nature communications, Vol. 8, pp. 13980, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).

Ji, Zhu, Gao, Zhang, Yan, Cen, Li, Zeng, Liao, Hou, Gao, Gao, Wei, Hui: "Baf60b-mediated ATM-p53 activation blocks cell identity conversion by sensing chromatin opening." in: Cell research, Vol. 27, Issue 5, pp. 642-656, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).

Yang, Chen, Deng, Jagannathan, Tobias, Schultz, Wang, Lengner, Rustgi, Lynch, Johnson: "Mutual reinforcement between telomere capping and canonical Wnt signalling in the intestinal stem cell niche." in: Nature communications, Vol. 8, pp. 14766, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).

Kalimutho, Bain, Mukherjee, Nag, Nanayakkara, Harten, Harris, Subramanian, Sinha, Shirasawa, Srihari, Burma, Khanna: "Enhanced dependency of KRAS-mutant colorectal cancer cells on RAD51-dependent homologous recombination repair identified from genetic interactions in Saccharomyces cerevisiae." in: Molecular oncology, Vol. 11, Issue 5, pp. 470-490, 2017 (PubMed).

Reynolds, Bicknell, Carroll, Higgs, Shaheen, Murray, Papadopoulos, Leitch, Murina, Tarnauskaitė, Wessel, Zlatanou, Vernet, von Kriegsheim, Mottram, Logan, Bye, Li, Brean, Maddirevula, Challis et al.: "Mutations in DONSON disrupt replication fork stability and cause microcephalic dwarfism. ..." in: Nature genetics, Vol. 49, Issue 4, pp. 537-549, 2017 (PubMed).

Tan, Nakajima, Wang, Sun, Xue, Wu, Hellwig, Zeng, Yates, Smithgall, Lei, Jiang, Levine, Su, Lan: "Nek7 Protects Telomeres from Oxidative DNA Damage by Phosphorylation and Stabilization of TRF1." in: Molecular cell, Vol. 65, Issue 5, pp. 818-831.e5, 2017 (PubMed).

Liu, Cussiol, Dibitetto, Sims, Twayana, Weiss, Freire, Marini, Pellicioli, Smolka: "TOPBP1(Dpb11) plays a conserved role in homologous recombination DNA repair through the coordinated recruitment of 53BP1(Rad9)." in: The Journal of cell biology, Vol. 216, Issue 3, pp. 623-639, 2017 (PubMed).

Nonnekens, Chatalic, Molkenboer-Kuenen, Beerens, Bruchertseifer, Morgenstern, Veldhoven-Zweistra, Schottelius, Wester, van Gent, van Weerden, Boerman, de Jong, Heskamp: "(213)Bi-Labeled Prostate-Specific Membrane Antigen-Targeting Agents Induce DNA Double-Strand Breaks in Prostate Cancer Xenografts." in: Cancer biotherapy & radiopharmaceuticals, Vol. 32, Issue 2, pp. 67-73, 2017 (PubMed).

Liu, Liu, Gao, Reske, Li, Wu, Yang, Chen, Luo, Xiao: "A single non-synonymous NCOA5 variation in type 2 diabetic patients with hepatocellular carcinoma impairs the function of NCOA5 in cell cycle regulation." in: Cancer letters, Vol. 391, pp. 152-161, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Viziteu, Klein, Basbous, Lin, Hirtz, Gourzones, Tiers, Bruyer, Vincent, Grandmougin, Seckinger, Goldschmidt, Constantinou, Pasero, Hose, Moreaux: "RECQ1 helicase is involved in replication stress survival and drug resistance in multiple myeloma." in: Leukemia, Vol. 31, Issue 10, pp. 2104-2113, 2017 (PubMed).

Vancevska, Douglass, Pfeiffer, Manley, Lingner: "The telomeric DNA damage response occurs in the absence of chromatin decompaction." in: Genes & development, Vol. 31, Issue 6, pp. 567-577, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Cottineau, Kottemann, Lach, Kang, Vély, Deenick, Lazarov, Gineau, Wang, Farina, Chansel, Lorenzo, Piperoglou, Ma, Nitschke, Belkadi, Itan, Boisson, Jabot-Hanin, Picard, Bustamante, Eidenschenk et al.: "Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. ..." in: The Journal of clinical investigation, Vol. 127, Issue 5, pp. 1991-2006, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Latella, DallAgnese, Sesillo, Nardoni, Cosentino, Lahm, Sacco, Puri: "DNA damage signaling mediates the functional antagonism between replicative senescence and terminal muscle differentiation." in: Genes & development, Vol. 31, Issue 7, pp. 648-659, 2017 (PubMed).

Hilmi, Jangal, Marques, Zhao, Saad, Zhang, Luo, Syme, Rejon, Yu, Krum, Fabian, Richard, Alaoui-Jamali, Orthwein, McCaffrey, Witcher: "CTCF facilitates DNA double-strand break repair by enhancing homologous recombination repair." in: Science advances, Vol. 3, Issue 5, pp. e1601898, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Garzón, Rodríguez, Kong, Chabes, Rodríguez-Acebes, Méndez, Moreno, García-Higuera: "Shortage of dNTPs underlies altered replication dynamics and DNA breakage in the absence of the APC/C cofactor Cdh1." in: Oncogene, 2017 (PubMed). (Sample species: Mouse (Murine)).

Yosef, Pilpel, Papismadov, Gal, Ovadya, Vadai, Miller, Porat, Ben-Dor, Krizhanovsky: "p21 maintains senescent cell viability under persistent DNA damage response by restraining JNK and caspase signaling." in: The EMBO journal, Vol. 36, Issue 15, pp. 2280-2295, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Mikuła-Pietrasik, Uruski, Pakuła, Maksin, Szubert, Woźniak, Naumowicz, Szpurek, Tykarski, Książek: "Oxidative stress contributes to hepatocyte growth factor-dependent pro-senescence activity of ovarian cancer cells." in: Free radical biology & medicine, Vol. 110, pp. 270-279, 2017 (PubMed).

Ha, Ma, Lin, Tang, Lian, Zhao, Li, Zhen, Pei, Han, Malumbres, Jin, Chen, Zhao, Zhu, Zhang: "The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites." in: Nature communications, Vol. 8, pp. 15751, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).