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Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody

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(140), (57), (37), (1)
(112), (24), (3), (1)
(3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(87), (50), (49), (38), (24), (23), (14), (11), (7), (6), (3), (2), (1), (1)
Pubmed 3 references available
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Quantity 0.1 mL
Shipping to United States ( )
Immunogen The epitope recognized by this antibody maps to a region between residues 350 and 400 of human 53BP1
Cross-Reactivity (Details) Human has been tested in WB, IHC-P,ICC/IF, mouse,goat have only been tested in ICC/IF. Primate reactivity reported in scientific literature (PMID: 18389475).
Purification affinity purified
Alternative Name TP53BP1 (TP53BP1 Antibody Abstract)
Background P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putativesubstrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes tothe presumptive sites of damage, specifically, double-strand breaks. This also suggestsa role in DNA repair, maintaining genomic stability. Alternate Names: anti-53 Binding Protein 1 antibody, anti-53BP-1 antibody, anti-TP53BP1 antibody,anti-Tumor Protein p53 Binding Protein 1 antibody.
Gene Symbol: TP53BP1
Gene ID 7158
Research Area Transcription Factors
Pathways DNA Damage Repair
Application Notes This 53BP1 antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry and Flow Cytometry. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunohistochemistry-Paraffin and Immunohistochemistry were reported in scientific literature. Frozen section data from customer review. Use in chromatin immunoprecipitation reported in scientific literature (PMID 24591601)
Recommended dilutions: Chromatin Immunoprecipitation, Flow Cytometry 1.5 µg/mL, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1:400-1:2000, Western Blot 1:5000-1:25000
Protocol Immunohistochemistry Protocol Specific for NB100-304: 53BP1 Antibody Materials1) 1 Phosphate buffered saline (pH 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/L2) Citrate buffer, 0.01 M, pH6.0, Sodium Citrate 3g, Citric acid 0.4g3) 3 % Hydrogen peroxide4) Primary antibody5) Blocking serum (normal serum)6) Biotinylated secondary antibody7) DAB staining kitMethods
1. Dewax and hydration of slides using xylene and EtOH:Dry slides for 20 min in a 60 C ovenAdd Xylene, 2 x 10 min100 %, 95 %, 80 %, and 70 % EtOH, 5 min each EtOH concentration Rinse in PBS, 5'2 Antigen retrieval method (only for paraffin slides)1a. High-pressure antigen retrieval procedure (recommended method)Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 mL of Citrate buffer (0.01M, pH6.
0) within a pressure cookerOnce steam is produced, and ONLY when steam is visible, from the pressure cooker (usually 15-20 min), the required high-pressure will have been reached, and slides will be incubated for 2 min.Turn off heat, and allow buffer and slides to cool to room temperatureSlides are then rinsed in PBS for 5 minutes
. Add 3 % hydrogen peroxide solution, 10'at RT, then PBS, 3X5'
. Normal blocking serum, 20'at RT
. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C
. Rinse with PBS, 3 X 5' each rinse
. Add Biotin-conjugated second antibody, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Add Streptavidin-Peroxidase, 10'at RT
. Rinse with PBS, 3 X 5' each rinse
. Staining with DAB solution, 2-5'under microscope
. Stop the reaction by washing in tap water
. Counterstain in Haematoxylin for 3-5 minutes
. 75 %, 80 %, 95 % and 100 % ethanol, 5x2', xylene 2 x 10'
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Immunofluorescence (IF) image for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody (ABIN151770) anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody
Immunofluorescence (IF) image for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody (ABIN151770) anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody (Image 2)
Western Blotting (WB) image for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody (ABIN151770) anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody (Image 3)
Product cited in: Konishi, de Lange: "Cell cycle control of telomere protection and NHEJ revealed by a ts mutation in the DNA-binding domain of TRF2." in: Genes & development, Vol. 22, Issue 9, pp. 1221-30, 2008 (PubMed).

Hockemeyer, Palm, Else et al.: "Telomere protection by mammalian Pot1 requires interaction with Tpp1." in: Nature structural & molecular biology, Vol. 14, Issue 8, pp. 754-61, 2007 (PubMed).

Riballo, Kühne, Rief et al.: "A pathway of double-strand break rejoining dependent upon ATM, Artemis, and proteins locating to gamma-H2AX foci." in: Molecular cell, Vol. 16, Issue 5, pp. 715-24, 2004 (PubMed).

Catalog No. ABIN151770

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