TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (C-Term)

Details for Product anti-TP53BP1 Antibody No. ABIN151771, Supplier: Log in to see
Antigen
  • TP53BP1
  • MGC160112
  • 53BP1
  • Tp53bp1
  • m53BP1
  • p202
  • Trp53bp1
  • tumor protein p53 binding protein 1
  • tumor protein p53 binding protein 1 (predicted), 5 prime
  • transformation related protein 53 binding protein 1
  • TP53BP1
  • tp53bp1
  • Trp53bp1
  • Tp53bp1
Alternatives
anti-Human TP53BP1 antibody for Western Blotting
Epitope
C-Term
47
28
18
16
16
10
8
7
4
4
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
238
153
68
19
19
13
12
4
4
4
3
2
2
1
Host
Rabbit
206
28
3
1
Clonality
Polyclonal
Conjugate
This TP53BP1 antibody is un-conjugated
10
10
8
8
8
7
7
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA), Western Blotting (WB)
179
90
88
82
81
49
40
27
24
12
7
5
5
2
2
1
Options
Supplier
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Supplier Product No.
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Immunogen The epitope recognized by this antibody maps to a region between residues 1925 and the C-terminus (residue 1972) of human 53BP1 (NP_005648.1).
Purification Immunogen affinity purified
Alternative Name 53BP1 (TP53BP1 Antibody Abstract)
Background Gene Symbol: TP53BP1
Gene ID 7158
NCBI Accession NP_005648
Pathways DNA Damage Repair
Application Notes Western Blot 1:2000-1:10000, Chromatin Immunoprecipitation, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen, Proximity Ligation Assay 1:1000This 53BP1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western Blot. Chromatin Immunoprecipitation and Immunohistochemistry-Frozen were reported in scientific literature.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for 53BP1 Antibody IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changesII. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation styleB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (C-Term) antibody (ABIN151771) Western Blot analysis of human 53BP1, using ABIN151771. Sample: Whole cell lysate (20...
Immunohistochemistry (IHC) image for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (C-Term) antibody (ABIN151771) Placenta, Villi 40X
Immunofluorescence (IF) image for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (C-Term) antibody (ABIN151771) Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [ABIN151771] - HeLa cells were...
Immunofluorescence (IF) image for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (C-Term) antibody (ABIN151771) Immunocytochemistry/Immunofluorescence: 53BP1 Antibody [ABIN151771] - Analysis in HeL...
Product cited in: Reindl, Girst, Walsh, Greubel, Schwarz, Siebenwirth, Drexler, Friedl, Dollinger: "Chromatin organization revealed by nanostructure of irradiation induced γH2AX, 53BP1 and Rad51 foci." in: Scientific reports, Vol. 7, pp. 40616, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Santaguida, Richardson, Iyer, MSaad, Zasadil, Knouse, Wong, Rhind, Desai, Amon: "Chromosome Mis-segregation Generates Cell-Cycle-Arrested Cells with Complex Karyotypes that Are Eliminated by the Immune System." in: Developmental cell, Vol. 41, Issue 6, pp. 638-651.e5, 2017 (PubMed).

Vancevska, Douglass, Pfeiffer, Manley, Lingner: "The telomeric DNA damage response occurs in the absence of chromatin decompaction." in: Genes & development, Vol. 31, Issue 6, pp. 567-577, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Cristini, Park, Capranico, Legube, Favre, Sordet: "DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions." in: Nucleic acids research, Vol. 44, Issue 3, pp. 1161-78, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Bakr, Köcher, Volquardsen, Reimer, Borgmann, Dikomey, Rothkamm, Mansour: "Functional crosstalk between DNA damage response proteins 53BP1 and BRCA1 regulates double strand break repair choice." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 119, Issue 2, pp. 276-81, 2016 (PubMed).

Szczepanski, Hussain, Sur, Altmüller, Thiele, Abdullah, Waseem, Moawia, Nürnberg, Noegel, Baig, Nürnberg: "A novel homozygous splicing mutation of CASC5 causes primary microcephaly in a large Pakistani family." in: Human genetics, Vol. 135, Issue 2, pp. 157-70, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Kibe, Zimmermann, de Lange: "TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres." in: Molecular cell, Vol. 61, Issue 2, pp. 236-46, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Penterling, Drexler, Böhland, Stamp, Wilke, Braselmann, Caldwell, Reindl, Girst, Greubel, Siebenwirth, Mansour, Borgmann, Dollinger, Unger, Friedl: "Depletion of Histone Demethylase Jarid1A Resulting in Histone Hyperacetylation and Radiation Sensitivity Does Not Affect DNA Double-Strand Break Repair." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156599, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Ochs, Somyajit, Altmeyer, Rask, Lukas, Lukas: "53BP1 fosters fidelity of homology-directed DNA repair." in: Nature structural & molecular biology, Vol. 23, Issue 8, pp. 714-21, 2016 (PubMed).

Hashizume, Zhang, Mueller, Prados, Lulla, Goldman, Saratsis, Mazar, Stegh, Cheng, Horbinski, Haas-Kogan, Sarkaria, Waldman, James: "Inhibition of DNA damage repair by the CDK4/6 inhibitor palbociclib delays irradiated intracranial atypical teratoid rhabdoid tumor and glioblastoma xenograft regrowth." in: Neuro-oncology, Vol. 18, Issue 11, pp. 1519-1528, 2016 (PubMed). Further details: Immunohistochemistry

Morales, Richard, Patidar, Motea, Dang, Manley, Boothman: "XRN2 Links Transcription Termination to DNA Damage and Replication Stress." in: PLoS genetics, Vol. 12, Issue 7, pp. e1006107, 2016 (PubMed).

Weyemi, Redon, Choudhuri, Aziz, Maeda, Boufraqech, Parekh, Sethi, Kasoji, Abrams, Merchant, Rajapakse, Bonner: "The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition." in: Nature communications, Vol. 7, pp. 10711, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Benkafadar, Menardo, Bourien, Nouvian, François, Decaudin, Maiorano, Puel, Wang: "Reversible p53 inhibition prevents cisplatin ototoxicity without blocking chemotherapeutic efficacy." in: EMBO molecular medicine, Vol. 9, Issue 1, pp. 7-26, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Croco, Marchionni, Bocchini, Angeloni, Stamato, Stefanelli, Hrelia, Sell, Lorenzini: "DNA Damage Detection by 53BP1: Relationship to Species Longevity." in: The journals of gerontology. Series A, Biological sciences and medical sciences, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Mutschelknaus, Peters, Winkler, Yentrapalli, Heider, Atkinson, Moertl: "Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation." in: PLoS ONE, Vol. 11, Issue 3, pp. e0152213, 2016 (PubMed).

van Ree, Nam, Jeganathan, Kanakkanthara, van Deursen: "Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes." in: Nature cell biology, Vol. 18, Issue 7, pp. 814-21, 2016 (PubMed).

Bakr, Köcher, Volquardsen, Petersen, Borgmann, Dikomey, Rothkamm, Mansour: "Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1-dependent end joining in G1." in: Oncotarget, Vol. 7, Issue 36, pp. 57679-57693, 2016 (PubMed).

Chen, Zhu, Zou, Xing, Gilson, Lu, Ye: "The topoisomerase II catalytic inhibitor ICRF-193 preferentially targets telomeres that are capped by TRF2." in: American journal of physiology. Cell physiology, Vol. 308, Issue 5, pp. C372-7, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Walter, Lier, Geiselhart, Thalheimer, Huntscha, Sobotta, Moehrle, Brocks, Bayindir, Kaschutnig, Muedder, Klein, Jauch, Schroeder, Geiger, Dick, Holland-Letz, Schmezer, Lane, Rieger, Essers, Williams et al.: "Exit from dormancy provokes DNA-damage-induced attrition in haematopoietic stem cells. ..." in: Nature, Vol. 520, Issue 7548, pp. 549-52, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Boersma, Moatti, Segura-Bayona, Peuscher, van der Torre, Wevers, Orthwein, Durocher, Jacobs: "MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end resection." in: Nature, Vol. 521, Issue 7553, pp. 537-40, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

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