Tumor Protein P53 Binding Protein 1 (TP53BP1) antibody

Antigen: Tumor Protein P53 Binding Protein 1 (TP53BP1)

Synonyms: p202, 53BP1, FLJ41424, MGC138366, Trp53bp1, TP53BP1, MGC160112

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN151771

Additional Information

Alternative name: TP53BP1

Gene ID: 7158

NCBI Acession: NP_0056481

Immunogen: The epitope recognized by this antibody maps to a region between residues 1925 andthe C-terminus (residue 1972) of human tumor protein p53 binding protein 1 using thenumbering given in entry NP_005648.1 (GeneID 7158).

Cross-Reactivity: Human

Description: P53-binding protein 1 (53BP1) plays a critical role in tumor suppression and is a putativesubstrate of ATM kinase. Upon DNA damage, it is phosphorylated and relocalizes tothe presumptive sites of damage, specifically, double-strand breaks. This also suggestsa role in DNA repair, maintaining genomic stability. Alternate Names: anti-53BP1 antibody, anti-53BP-1 antibody, anti-TP53BP1 antibody, anti-Tumor Proteinp53 Binding Protein 1 antibody.

Specificity: ABIN151771 is specific for human 53BP1 protein. Species Reactivity: Human and Mouse in Western Blot and immunocytochemistry/immunofluorescence andit reacts with Mouse in immunocytochemistry/immunofluorescence (Western Blot hasnot been done using this antibody on Mouse protein).

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:200- 1:10000,Immunocytochemistry 1:200-1:1000. Positive Controls: U205, 293T, and MO59K cell lysates (human) and MEF lysates (mouse)

Concentration: 1 mg/ml

Purification: Aff - Purified

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8. Preservative: 0.09% Sodium Azide.

Storage: Store at 4C. Do not freeze.

Research Area: Transcription Factors

Restrictions: For Research Use only

Images

Western Blot analysis of human 53BP1, using ABIN151771. Sample: Whole cell lysate (20 ug/lane) from U2Os cells resolved on a 3 to 8% trisacetate gel.

Placenta, Villi 40X

Publications

Silverman, Takai, Buonomo et al.: "Human Rif1, ortholog of a yeast telomeric protein, is regulated by ATM and 53BP1 and functions in the S-phase checkpoint." in: Genes & development, Vol. 18, Issue 17, pp. 2108-19, 2004 (PubMed).

Rothkamm, Balroop, Shekhdar et al.: "Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure." in: Radiology, Vol. 242, Issue 1, pp. 244-51, 2006 (PubMed).

Ibarra, Schwob, Muendez: "Excess MCM proteins protect human cells from replicative stress by licensing backup origins of replication." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 26, pp. 8956-61, 2008 (PubMed).

Guirouilh-Barbat, Redon, Pommier: "Transcription-coupled DNA double-strand breaks are mediated via the nucleotide excision repair and the Mre11-Rad50-Nbs1 complex." in: Molecular biology of the cell, Vol. 19, Issue 9, pp. 3969-81, 2008 (PubMed).

Wirthner, Wrann, Balamurugan et al.: "Impaired DNA double-strand break repair contributes to chemoresistance in HIF-1 alpha-deficient mouse embryonic fibroblasts." in: Carcinogenesis, Vol. 29, Issue 12, pp. 2306-16, 2008 (PubMed).

Palm, Hockemeyer, Kibe et al.: "Functional dissection of human and mouse POT1 proteins." in: Molecular and cellular biology, Vol. 29, Issue 2, pp. 471-82, 2008 (PubMed).

Solier, Sordet, Kohn et al.: "Death receptor-induced activation of the Chk2- and histone H2AX-associated DNA damage response pathways." in: Molecular and cellular biology, Vol. 29, Issue 1, pp. 68-82, 2008 (PubMed).

Spardy, Duensing, Hoskins et al.: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008 (PubMed).