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TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (C-Term)

Details for Product anti-TP53BP1 Antibody No. ABIN151771, Supplier: Log in to see
Antigen
  • 53BP1
  • m53BP1
  • MGC160112
  • p202
  • Tp53bp1
  • TP53BP1
  • Trp53bp1
Alternatives
anti-Human TP53BP1 antibody for Western Blotting
Epitope
C-Term
25
24
14
14
13
8
8
7
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
202
103
56
9
9
9
1
Host
Rabbit
172
26
3
1
Clonality
Polyclonal
Conjugate
This TP53BP1 antibody is un-conjugated
7
7
7
7
6
6
5
5
4
4
4
2
2
2
2
2
2
2
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA), Western Blotting (WB)
149
67
61
57
50
41
27
23
9
7
7
4
3
2
1
Supplier
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Available images

Immunogen The epitope recognized by this antibody maps to a region between residues 1925 and the C-terminus (residue 1972) of human 53BP1 (NP_005648.1).
Cross-Reactivity (Details) Human has been tested in both WB,ICC/IF, mouse has only been tested in ICC/IF. rat,reactivity reported in scientific literature (PMID: 24244353)
Purification Immunogen affinity purified
Alternative Name 53BP1 (TP53BP1 Antibody Abstract)
Background Gene Symbol: TP53BP1
NCBI Accession NP_005648
Pathways DNA Damage Repair
Application Notes Western Blot 1:2000-1:10000, Chromatin Immunoprecipitation, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:1000, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen, Proximity Ligation Assay 1:1000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for 53BP1 Antibody IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changesII. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation styleB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (C-Term) (ABIN151771) Western Blot analysis of human 53BP1, using ABIN151771. Sample: Whole cell lysate (20...
Immunohistochemistry (IHC) image for anti-TP53BP1 antibody (Tumor Protein P53 Binding Protein 1) (C-Term) (ABIN151771) Placenta, Villi 40X
Product cited in: Cristini, Park, Capranico, Legube, Favre, Sordet: "DNA-PK triggers histone ubiquitination and signaling in response to DNA double-strand breaks produced during the repair of transcription-blocking topoisomerase I lesions." in: Nucleic acids research, Vol. 44, Issue 3, pp. 1161-78, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Bakr, Köcher, Volquardsen, Reimer, Borgmann, Dikomey, Rothkamm, Mansour: "Functional crosstalk between DNA damage response proteins 53BP1 and BRCA1 regulates double strand break repair choice." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 119, Issue 2, pp. 276-81, 2016 (PubMed).

Szczepanski, Hussain, Sur, Altmüller, Thiele, Abdullah, Waseem, Moawia, Nürnberg, Noegel, Baig, Nürnberg: "A novel homozygous splicing mutation of CASC5 causes primary microcephaly in a large Pakistani family." in: Human genetics, Vol. 135, Issue 2, pp. 157-70, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Kibe, Zimmermann, de Lange: "TPP1 Blocks an ATR-Mediated Resection Mechanism at Telomeres." in: Molecular cell, Vol. 61, Issue 2, pp. 236-46, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Penterling, Drexler, Böhland, Stamp, Wilke, Braselmann, Caldwell, Reindl, Girst, Greubel, Siebenwirth, Mansour, Borgmann, Dollinger, Unger, Friedl: "Depletion of Histone Demethylase Jarid1A Resulting in Histone Hyperacetylation and Radiation Sensitivity Does Not Affect DNA Double-Strand Break Repair." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156599, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Ochs, Somyajit, Altmeyer, Rask, Lukas, Lukas: "53BP1 fosters fidelity of homology-directed DNA repair." in: Nature structural & molecular biology, Vol. 23, Issue 8, pp. 714-21, 2016 (PubMed).

Hashizume, Zhang, Mueller, Prados, Lulla, Goldman, Saratsis, Mazar, Stegh, Cheng, Horbinski, Haas-Kogan, Sarkaria, Waldman, James: "Inhibition of DNA damage repair by the CDK4/6 inhibitor palbociclib delays irradiated intracranial atypical teratoid rhabdoid tumor and glioblastoma xenograft regrowth." in: Neuro-oncology, Vol. 18, Issue 11, pp. 1519-1528, 2016 (PubMed). Further details: Immunohistochemistry

Morales, Richard, Patidar, Motea, Dang, Manley, Boothman: "XRN2 Links Transcription Termination to DNA Damage and Replication Stress." in: PLoS genetics, Vol. 12, Issue 7, pp. e1006107, 2016 (PubMed).

Chen, Zhu, Zou, Xing, Gilson, Lu, Ye: "The topoisomerase II catalytic inhibitor ICRF-193 preferentially targets telomeres that are capped by TRF2." in: American journal of physiology. Cell physiology, Vol. 308, Issue 5, pp. C372-7, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Walter, Lier, Geiselhart, Thalheimer, Huntscha, Sobotta, Moehrle, Brocks, Bayindir, Kaschutnig, Muedder, Klein, Jauch, Schroeder, Geiger, Dick, Holland-Letz, Schmezer, Lane, Rieger, Essers, Williams et al.: "Exit from dormancy provokes DNA-damage-induced attrition in haematopoietic stem cells. ..." in: Nature, Vol. 520, Issue 7548, pp. 549-52, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Boersma, Moatti, Segura-Bayona, Peuscher, van der Torre, Wevers, Orthwein, Durocher, Jacobs: "MAD2L2 controls DNA repair at telomeres and DNA breaks by inhibiting 5' end resection." in: Nature, Vol. 521, Issue 7553, pp. 537-40, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Mathieu, Verronese, Rice, Fouyssac, Bertrand, Picard, Chansel, Walter, Notarangelo, Butte, Nadeau, Csomos, Chen, Chen, Delgado, Rigal, Bardin, Schuetz, Moshous, Reumaux, Plenat, Phan, Zabot, Balme et al.: "PRKDC mutations associated with immunodeficiency, granuloma, and autoimmune regulator-dependent autoimmunity. ..." in: The Journal of allergy and clinical immunology, Vol. 135, Issue 6, pp. 1578-88.e5, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Reindl, Drexler, Girst, Greubel, Siebenwirth, Drexler, Dollinger, Friedl: "Nanoscopic exclusion between Rad51 and 53BP1 after ion irradiation in human HeLa cells." in: Physical biology, Vol. 12, Issue 6, pp. 66005, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Yi, Park, Kwak, Seong: "Co-treatment with BEZ235 Enhances Sensitivity of BRCA1-negative Breast Cancer Cells to Olaparib." in: Anticancer research, Vol. 35, Issue 7, pp. 3829-38, 2015 (PubMed). (Sample species: Human).

Belin, Lee, Mullins: "DNA damage induces nuclear actin filament assembly by Formin-2 and Spire-½ that promotes efficient DNA repair." in: eLife, Vol. 4, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Kato, Nakajima, Ui, Muto-Terao, Ogiwara, Nakada: "Fine-tuning of DNA damage-dependent ubiquitination by OTUB2 supports the DNA repair pathway choice." in: Molecular cell, Vol. 53, Issue 4, pp. 617-30, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Münch, Weidtkamp-Peters, Klement, Grigaravicius, Monajembashi, Salomoni, Pandolfi, Weißhart, Hemmerich: "The tumor suppressor PML specifically accumulates at RPA/Rad51-containing DNA damage repair foci but is nonessential for DNA damage-induced fibroblast senescence." in: Molecular and cellular biology, Vol. 34, Issue 10, pp. 1733-46, 2014 (PubMed).

Clynes, Jelinska, Xella, Ayyub, Taylor, Mitson, Bachrati, Higgs, Gibbons: "ATRX dysfunction induces replication defects in primary mouse cells." in: PLoS ONE, Vol. 9, Issue 3, pp. e92915, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Lu, Fang, Sykora, Kulikowicz, Zhang, Becker, Croteau, Bohr: "Senescence induced by RECQL4 dysfunction contributes to Rothmund-Thomson syndrome features in mice." in: Cell death & disease, Vol. 5, pp. e1226, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Klement, Goodarzi: "DNA double strand break responses and chromatin alterations within the aging cell." in: Experimental cell research, Vol. 329, Issue 1, pp. 42-52, 2014 (PubMed).