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Ataxia Telangiectasia Mutated (ATM) (pSer1981) antibody

Details for Product No. ABIN151772
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Antigen
Synonyms ATM, Atm, CG6535, Dmel\\CG6535, Tefu, atm, atm/tefu, dATM, tef, Xatm, at1, atdc, tel1, telo1, AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1, AI256621, C030026E19Rik
Epitope
pSer1981
(55), (19), (12), (12), (8), (8), (5), (5), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(226), (67), (39), (4), (4), (1), (1)
Host
Mouse
(164), (44), (16), (6), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(128), (61), (50), (46), (45), (40), (33), (13), (8), (5), (2), (2)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
Catalog No. ABIN151772
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  • +1 404 474 4654
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Immunogen Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM.
Clone 10H11-E12
Isotype IgG1 kappa
No Cross-Reactivity Dog (Canine)
Cross-Reactivity (Details) Does not work in canine per customer review
Purification Protein G purified
Alternative Name ATM
Background ATM-kinase plays an important regulatory role. DNA damage caused by such things asionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions thatregulate cell cycle, apoptosis, and DNA damage repair. Alternate Names: anti-phosphorylated ATM antibody, anti-ATM S1981 antibody, anti-ATM phosphorylatedSer 1981 antibody, anti-ATM (phospho S1981) antibody.
Gene Symbol: ATM
Gene ID 472, 300711
UniProt Q13315
Application Notes This ATM [p Ser1981] (10H11.E12) antibody useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band at ~370 kDa can be seen. In IHC-P, staining was observed in the nucleus and cytoplasm of mouse spleen.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry 1:200, Immunohistochemistry-Paraffin 1:200, Immunoprecipitation 1:10-1:500, Western Blot 1:1000
Protocol Protocol specific for ATM Kinase Antibody Western Blot Procedure1) Resolve protein samples on a 6 % SDS-PAGE gel at 185V for ~1.5 hours.
2) Transfer to PVDF membranes at 25V for ~1.5 hours.
) Block the membrane with TBST+BSA and goat serum for 1 hour at RT.
) Dilute primary antibody to 1:1,000 in blocking buffer.
) Incubate membrane overnight at 4 degrees Celcius in diluted anti-ATM-kinase.
) Wash 3 times ten minutes on a shaker.
) Incubate membranes with HRP conjugated anti-mouse IgG for 1 hour (RT), diluted in blocking buffer.
) Wash 3 times ten minutes on a shaker.
) Add ECL reagent, as per kit directions, and expose for 1-5 seconds.
Restrictions For Research Use only
Format Liquid
Concentration 0.97 mg/mL
Buffer Tris-glycine, 150 mM NaCl, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Ataxia Telangiectasia Mutated (ATM) (pSer1981) antibody Western Blot analysis of ATM-kinase, using ABIN151772. Sample: Irradiated or peroxidated human fibroblasts.
General Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003 (PubMed).

Yalcin, Zhang, Luciano et al.: "Foxo3 is essential for the regulation of ataxia telangiectasia mutated and oxidative stress-mediated homeostasis of hematopoietic stem cells." in: The Journal of biological chemistry, Vol. 283, Issue 37, pp. 25692-705, 2008 (PubMed).

Spardy, Duensing, Hoskins et al.: "HPV-16 E7 reveals a link between DNA replication stress, fanconi anemia D2 protein, and alternative lengthening of telomere-associated promyelocytic leukemia bodies." in: Cancer research, Vol. 68, Issue 23, pp. 9954-63, 2008 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN151772
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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