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ATM antibody (Ataxia Telangiectasia Mutated) (pSer1981)

Details for Product anti-ATM Antibody No. ABIN151773, Supplier: Login to see New
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(76), (22), (17), (16), (13), (13), (8), (8), (7), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(303), (75), (30), (6), (4), (1), (1)
(218), (58), (16), (9), (4)
Clonality (Clone)
Monoclonal ()
This ATM antibody is un-conjugated
(8), (8), (6), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
(175), (79), (71), (52), (44), (43), (25), (22), (16), (11), (7), (5), (4), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Quantity 0.1 mL
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Immunogen Synthetic peptide made to a region surrounding the phosphorylated Serine 1981 of human ATM.
Clone 10H11-E12
Isotype IgG1 kappa
Purification Ascites
Alternative Name ATM (ATM Antibody Abstract)
Background ATM-kinase plays an important regulatory role. DNA damage caused by such things asionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions thatregulate cell cycle, apoptosis, and DNA damage repair. Alternate Names: anti-phosphorylated ATM antibody, anti-ATM S1981 antibody, anti-ATM phosphorylatedSer 1981 antibody.
Gene Symbol: ATM
Gene ID 472
UniProt Q13315
Pathways p53 Signaling, Apoptosis, DNA Damage Repair
Application Notes This ATM [p Ser1981] (10H11.E12) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a single band is seen at ~370 kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:500, Western Blot 1:1000
Protocol Western Blot Protocol for ATM Antibody 1) Resolve protein samples on a 6 % SDS-PAGE gel at 185V for ~1.5 hours.
2) Transfer to PVDF membranes at 25V for ~1.5 hours.
) Block the membrane with TBST+BSA and goat serum for 1 hour at RT.
) Dilute purified primary antibody to 1:1000 in blocking buffer.
) Incubate membrane overnight at 4 degrees Celcius in diluted purified anti-ATM.
) Wash 3 times ten minutes on a shaker.
) Incubate membranes with HRP conjugated anti-mouse IgG for 1 hour (RT), diluted in blocking buffer.
) Wash 3 times ten minutes on a shaker.
) Add ECL reagent, as per kit directions, and expose for 1-5 seconds.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunocytochemistry Protocol for ATM Antibody Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
General Chu, Yang, Lu et al.: "Silencing of p29 affects DNA damage responses with UV irradiation." in: Cancer research, Vol. 66, Issue 17, pp. 8484-91, 2006 (PubMed).

Bakkenist, Kastan: "DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation." in: Nature, Vol. 421, Issue 6922, pp. 499-506, 2003 (PubMed).

Bartek, Lukas: "DNA repair: Damage alert." in: Nature, Vol. 421, Issue 6922, pp. 486-8, 2003 (PubMed).

Catalog No. ABIN151773

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