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ATR antibody

Details for Product No. ABIN151774, Supplier: Log in to see
Antigen
  • 9130222L19Rik
  • ATR
  • FCTCS
  • FRP1
  • MEC1
  • SCKL
  • SCKL1
Reactivity
Human
120
22
15
7
3
1
1
1
1
1
Host
Mouse
93
25
9
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
81
62
40
23
19
19
13
9
2
1
Supplier
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Available images

Immunogen Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.
Clone 2B5
Isotype IgG
Purification Protein G purified
Background Gene Symbol: ATR
Molecular Weight Theoretical MW: 317 kDa
UniProt Q13535
Research Area DNA/RNA, Cell Cycle, Cancer
Application Notes Western Blot 1:500-1:1000, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 1:10-1:500, Functional
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-ATR antibody (ABIN151774) Western Blot analysis of ATR, using ABIN151774. Sample: Raji whole cell extracts.
Product cited in: Oku, Nakano, Kaneko, Ando, Kenmotsu, Itoh, Tsuiji, Seyama, Toyoshima, Tsuji: "Constitutive turnover of phosphorylation at Thr-412 of human p57/coronin-1 regulates the interaction with actin." in: The Journal of biological chemistry, Vol. 287, Issue 51, pp. 42910-20, 2012 (PubMed). (Sample species: Human).

Haro, Sheth, Scheinberg: "Dysregulation of IRP1-mediated iron metabolism causes gamma ray-specific radioresistance in leukemia cells." in: PLoS ONE, Vol. 7, Issue 11, pp. e48841, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Chen, Chen, Riley, Chen: "Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR." in: Cell cycle (Georgetown, Tex.), Vol. 10, Issue 4, pp. 655-63, 2011 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Ha, Fiskus, Rao, Balusu, Venkannagari, Nalabothula, Bhalla: "Hsp90 inhibitor-mediated disruption of chaperone association of ATR with hsp90 sensitizes cancer cells to DNA damage." in: Molecular cancer therapeutics, Vol. 10, Issue 7, pp. 1194-206, 2011 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Zuco, Benedetti, Zunino: "ATM- and ATR-mediated response to DNA damage induced by a novel camptothecin, ST1968." in: Cancer letters, Vol. 292, Issue 2, pp. 186-96, 2010 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Cui, Johnson, Bullock, Ali-Osman, Bigner, Friedman: "Bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nitrosourea activates the ATR-Chk1 pathway independently of the mismatch repair pathway." in: Molecular pharmacology, Vol. 75, Issue 6, pp. 1356-63, 2009 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Kulkarni, Das: "Differential roles of ATR and ATM in p53, Chk1, and histone H2AX phosphorylation in response to hyperoxia: ATR-dependent ATM activation." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 294, Issue 5, pp. L998-L1006, 2008 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Katsuki, Nakada, Yokoyama, Imoto, Inazawa, Nagasawa, Mizutani: "Caffeine yields aneuploidy through asymmetrical cell division caused by misalignment of chromosomes." in: Cancer science, Vol. 99, Issue 8, pp. 1539-45, 2008 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Liu, Matsuda, Plunkett et al.: "Ataxia-telangiectasia and Rad3-related and DNA-dependent protein kinase cooperate in G2 checkpoint activation by the DNA strand-breaking nucleoside analogue ..." in: Molecular cancer therapeutics, Vol. 7, Issue 1, pp. 133-42, 2008 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Wu, Shell, Liu, Zou: "ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation." in: Oncogene, Vol. 26, Issue 5, pp. 757-64, 2007 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Manthey, Opiyo, Glanzer, Dimitrova, Elliott, Oakley: "NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse." in: Journal of cell science, Vol. 120, Issue Pt 23, pp. 4221-9, 2007 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Wu, Shell, Yang, Zou et al.: "Phosphorylation of nucleotide excision repair factor xeroderma pigmentosum group A by ataxia telangiectasia mutated and Rad3-related-dependent checkpoint pathway promotes cell survival in response to ..." in: Cancer research, Vol. 66, Issue 6, pp. 2997-3005, 2006 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Tyagi, Singh, Agarwal, Agarwal et al.: "Silibinin activates p53-caspase 2 pathway and causes caspase-mediated cleavage of Cip1/p21 in apoptosis induction in bladder transitional-cell papilloma RT4 cells: evidence for a regulatory loop ..." in: Carcinogenesis, Vol. 27, Issue 11, pp. 2269-80, 2006 (PubMed).

Liu, Rusinol, Sinensky, Wang, Zou: "DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A." in: Journal of cell science, Vol. 119, Issue Pt 22, pp. 4644-9, 2006 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Li, Stern: "Regulation of CHK2 by DNA-dependent protein kinase." in: The Journal of biological chemistry, Vol. 280, Issue 12, pp. 12041-50, 2005 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Horton, Stefanick, Naron, Kedar, Wilson: "Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure." in: The Journal of biological chemistry, Vol. 280, Issue 16, pp. 15773-85, 2005 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Gibson, Bindra, Glazer: "Hypoxia-induced phosphorylation of Chk2 in an ataxia telangiectasia mutated-dependent manner." in: Cancer research, Vol. 65, Issue 23, pp. 10734-41, 2005 (PubMed). (Sample species: Human). Further details: Immunoprecipitation,Functional Studies

Background publications Abraham: "Cell cycle checkpoint signaling through the ATM and ATR kinases." in: Genes & development, Vol. 15, Issue 17, pp. 2177-96, 2001 (PubMed).

Post, Weng, Cimprich, Chen, Xu, Lee: "Phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G(1)/S checkpoint activation in response to DNA damage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 23, pp. 13102-7, 2001 (PubMed).