NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) (AA 56-642) antibody

Details for Product No. ABIN151804
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Antigen
Synonyms CG9938, Dmel\\CG9938, Hec1, NDC80, dNdc80, dmNdc80, hec1, KNTC2, ndc10, tid3, 2610020P18Rik, AV139730, HEC, HEC1, Kntc2, kntc2l, wu:fb52e02, zgc:111801, HsHec1, TID3, hsNDC80, kntc2
Epitope
AA 56-642
(9), (8), (4), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(74), (28), (16), (12), (12), (12)
Host
Mouse
(52), (15), (7)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(61), (30), (17), (14), (13), (10), (6), (6), (4), (2), (2), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN151804
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Immunogen Human HEC1 protein consisting of amino acids 56-642.
Clone 9G3-23
Isotype IgG2b
Specificity HEC1 (9G3.23)
Cross-Reactivity (Details) May also react with Mouse, Pig,African green monkey. (not validated)
Purification Protein G purified
Alternative Name NDC80
Background Human HEC1 is a coiled-coil protein that has been demonstrated to have a role inM-phase progression. It is essential for proper chromosome segregation. Its depletionresults in the impairment of chromosome congression and results in spindle checkpointactivation. Alternate Names: anti-HEC antibody, anti-Highly Expressed in Cancer antibody, anti-KinetochoreAssociated 2 antibody, anti-KNTC2 antibody.
Gene Symbol: NDC80
Gene ID 10403
Application Notes Recommended dilutions: Immunocytochemistry/Immunofluorescence 4-10 µg/mL, Immunoprecipitation 1-5 µg/mL, Western Blot 1:250-1:1000
Restrictions For Research Use only
Format Liquid
Buffer PBS [pH 7.4]
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) (AA 56-642) antibody anti-NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) (AA 56-642) antibody
General Chen, Riley, Chen et al.: "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 6049-56, 1997 (PubMed).

Zheng, Chen, Riley et al.: "Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p." in: Molecular and cellular biology, Vol. 20, Issue 10, pp. 3529-37, 2000 (PubMed).

Martin-Lluesma, Stucke, Nigg: "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2." in: Science (New York, N.Y.), Vol. 297, Issue 5590, pp. 2267-70, 2002 (PubMed).

Ganem, Compton: "The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK." in: The Journal of cell biology, Vol. 166, Issue 4, pp. 473-8, 2004 (PubMed).

Ciferri, De Luca, Monzani et al.: "Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore." in: The Journal of biological chemistry, Vol. 280, Issue 32, pp. 29088-95, 2005 (PubMed).

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