NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) antibody

Antigen: NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80)

Synonyms: HEC, HEC1, TID3, KNTC2, hsNDC80, Kntc2, AV139730, 2610020P18Rik, CG9938, DmelCG9938, dmNdc80, Hec1, ndc80, wu:fb52e02, zgc:111801, NDC80

Clonality: Monoclonal (9G3-23)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN151804

Additional Information

Alternative name: NDC80

Gene ID: 10403

Immunogen: Recombinant protein encoding amino acids 56-642 of human HEC1 purified from E. coli.

Cross-Reactivity: Human

Isotype: IgG2b

Clone: 9G3-23

Description: Human HEC1 is a coiled-coil protein that has been demonstrated to have a role inM-phase progression. It is essential for proper chromosome segregation. Its depletionresults in the impairment of chromosome congression and results in spindle checkpointactivation. Alternate Names: anti-HEC antibody, anti-Highly Expressed in Cancer antibody, anti-KinetochoreAssociated 2 antibody, anti-KNTC2 antibody.

Specificity: Species Reactivity: ABIN151804 reacts with human. Other species have not been tested.

Application Details

Application Notes: Suggested working dilutions: immunofluorescence 4-10 ug/ml,immunoprecipitation 1-5 ug,Western Blot 1:500-1:1000

Concentration: 1 mg/ml

Purification: Purified

Buffer: PBS [pH7.4]. Preservative: No preservative.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

anti-NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) antibody

Publications

Chen, Riley, Chen et al.: "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 6049-56, 1997 (PubMed).

Zheng, Chen, Riley et al.: "Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p." in: Molecular and cellular biology, Vol. 20, Issue 10, pp. 3529-37, 2000 (PubMed).

Martin-Lluesma, Stucke, Nigg: "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2." in: Science (New York, N.Y.), Vol. 297, Issue 5590, pp. 2267-70, 2002 (PubMed).

Ganem, Compton: "The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK." in: The Journal of cell biology, Vol. 166, Issue 4, pp. 473-8, 2004 (PubMed).

Ciferri, De Luca, Monzani et al.: "Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore." in: The Journal of biological chemistry, Vol. 280, Issue 32, pp. 29088-95, 2005 (PubMed).