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NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) (AA 56-642) antibody

Details for Product No. ABIN151804, Supplier: Log in to see
Antigen
  • CG9938
  • Dmel\\CG9938
  • Hec1
  • NDC80
  • dNdc80
  • dmNdc80
  • hec1
  • KNTC2
  • ndc10
  • tid3
  • 2610020P18Rik
  • AV139730
  • HEC
  • HEC1
  • Kntc2
  • kntc2l
  • wu:fb52e02
  • zgc:111801
  • HsHec1
  • TID3
  • hsNDC80
  • kntc2
Epitope
AA 56-642
18
5
4
4
3
3
2
2
1
1
1
1
Reactivity
Human
77
19
10
3
2
2
Host
Mouse
42
19
16
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
67
29
14
12
9
8
7
3
3
2
2
1
1
Supplier
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Immunogen Human HEC1 protein consisting of amino acids 56-642.
Clone 9G3-23
Isotype IgG2b
Specificity HEC1 (9G3.23)
Cross-Reactivity (Details) May also react with Mouse, Pig,African green monkey. (not validated)
Purification Protein G purified
Alternative Name NDC80 (NDC80 Antibody Abstract)
Background Human HEC1 is a coiled-coil protein that has been demonstrated to have a role inM-phase progression. It is essential for proper chromosome segregation. Its depletionresults in the impairment of chromosome congression and results in spindle checkpointactivation. Alternate Names: anti-HEC antibody, anti-Highly Expressed in Cancer antibody, anti-KinetochoreAssociated 2 antibody, anti-KNTC2 antibody.
Gene Symbol: NDC80
Gene ID 10403
Application Notes Recommended dilutions: Immunocytochemistry/Immunofluorescence 4-10 µg/mL, Immunoprecipitation 1-5 µg/mL, Western Blot 1:250-1:1000
Restrictions For Research Use only
Format Liquid
Buffer PBS [pH 7.4]
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) (AA 56-642) antibody (ABIN151804) anti-NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) (AA 56-642) antibody
Product cited in: Ciferri, De Luca, Monzani et al.: "Architecture of the human ndc80-hec1 complex, a critical constituent of the outer kinetochore." in: The Journal of biological chemistry, Vol. 280, Issue 32, pp. 29088-95, 2005 (PubMed).

Ganem, Compton: "The KinI kinesin Kif2a is required for bipolar spindle assembly through a functional relationship with MCAK." in: The Journal of cell biology, Vol. 166, Issue 4, pp. 473-8, 2004 (PubMed).

Martin-Lluesma, Stucke, Nigg: "Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2." in: Science (New York, N.Y.), Vol. 297, Issue 5590, pp. 2267-70, 2002 (PubMed).

Zheng, Chen, Riley et al.: "Retinoblastoma protein enhances the fidelity of chromosome segregation mediated by hsHec1p." in: Molecular and cellular biology, Vol. 20, Issue 10, pp. 3529-37, 2000 (PubMed).

Background publications Chen, Riley, Chen et al.: "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 6049-56, 1997 (PubMed).