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NDC80 antibody (NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae))

Details for Product anti-NDC80 Antibody No. ABIN151804, Supplier: Log in to see
Antigen
  • 2610020P18Rik
  • AV139730
  • CG9938
  • Dmel\\CG9938
  • dmNdc80
  • dNdc80
  • HEC
  • hec1
  • HEC1
  • Hec1
  • HsHec1
  • hsNDC80
  • kntc2
  • KNTC2
  • Kntc2
  • kntc2l
  • ndc10
  • NDC80
  • tid3
  • TID3
  • wu:fb52e02
  • zgc:111801
Reactivity
Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
96
31
22
15
13
13
3
2
Host
Mouse
41
36
19
Clonality (Clone)
Monoclonal ()
Conjugate
This NDC80 antibody is un-conjugated
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
84
36
24
18
15
12
9
7
3
3
1
1
1
Supplier
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Immunogen Human HEC1 protein consisting of amino acids 56 - 642.
Clone 9G3-23
Isotype IgG2a
Cross-Reactivity (Details) May also react with Mouse, Pig,African green monkey. (not validated)
Purification Protein G purified
Alternative Name NDC80 (NDC80 Antibody Abstract)
Background Gene Symbol: NDC80
Molecular Weight Theoretical MW: 80 kDa
Gene ID 10403
UniProt O14777
Application Notes Western Blot 1:500 - 1:3000, Flow Cytometry 1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 4 - 10 μg/mL, Immunoprecipitation 1 - 5 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer PBS ( pH 7.4)
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-NDC80 antibody (NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae)) (ABIN151804) anti-NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) antibody
Product cited in: Tauchman, Boehm, DeLuca: "Stable kinetochore-microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells." in: Nature communications, Vol. 6, pp. 10036, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Yao, Kondoh, Natsume, Yamanaka, Inoue, Toki, Takagi, Shimizu, Yamori, Osada, Noda: "A small compound targeting TACC3 revealed its different spatiotemporal contributions for spindle assembly in cancer cells." in: Oncogene, Vol. 33, Issue 33, pp. 4242-52, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Sivakumar, Daum, Tipton, Rankin, Gorbsky: "The spindle and kinetochore-associated (Ska) complex enhances binding of the anaphase-promoting complex/cyclosome (APC/C) to chromosomes and promotes mitotic exit." in: Molecular biology of the cell, Vol. 25, Issue 5, pp. 594-605, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Kleyman, Kabeche, Compton: "STAG2 promotes error correction in mitosis by regulating kinetochore-microtubule attachments." in: Journal of cell science, Vol. 127, Issue Pt 19, pp. 4225-33, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Hwang, Peddibhotla, McHenry, Chang, Yochum, Park, Sears, Vargo-Gogola: "P190B RhoGAP Regulates Chromosome Segregation in Cancer Cells." in: Cancers, Vol. 4, Issue 2, pp. 475-89, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Maier, Kirsch, Anderhub, Zentgraf, Krämer: "The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells." in: Cell cycle (Georgetown, Tex.), Vol. 12, Issue 9, pp. 1457-71, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Kabeche, Compton: "Cyclin A regulates kinetochore microtubules to promote faithful chromosome segregation." in: Nature, Vol. 502, Issue 7469, pp. 110-3, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Chan, Jeyaprakash, Nigg, Santamaria: "Aurora B controls kinetochore-microtubule attachments by inhibiting Ska complex-KMN network interaction." in: The Journal of cell biology, Vol. 196, Issue 5, pp. 563-71, 2012 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

De Antoni, Maffini, Knapp, Musacchio, Santaguida: "A small-molecule inhibitor of Haspin alters the kinetochore functions of Aurora B." in: The Journal of cell biology, Vol. 199, Issue 2, pp. 269-84, 2012 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Krenn, Wehenkel, Li, Santaguida, Musacchio: "Structural analysis reveals features of the spindle checkpoint kinase Bub1-kinetochore subunit Knl1 interaction." in: The Journal of cell biology, Vol. 196, Issue 4, pp. 451-67, 2012 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Matson, Demirel, Stukenberg, Burke: "A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint." in: Genes & development, Vol. 26, Issue 6, pp. 542-7, 2012 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Sundin, Guimaraes, Deluca: "The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis." in: Molecular biology of the cell, Vol. 22, Issue 6, pp. 759-68, 2011 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Tooley, Miller, Stukenberg: "The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement." in: Molecular biology of the cell, Vol. 22, Issue 8, pp. 1217-26, 2011 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Screpanti, De Antoni, Alushin, Petrovic, Melis, Nogales, Musacchio: "Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore." in: Current biology : CB, Vol. 21, Issue 5, pp. 391-8, 2011 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Santaguida, Vernieri, Villa, Ciliberto, Musacchio: "Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction." in: The EMBO journal, Vol. 30, Issue 8, pp. 1508-19, 2011 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Barnhart, Kuich, Stellfox, Ward, Bassett, Black, Foltz: "HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore." in: The Journal of cell biology, Vol. 194, Issue 2, pp. 229-43, 2011 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Ma, Titus, Gable, Ross, Wadsworth: "TPX2 regulates the localization and activity of Eg5 in the mammalian mitotic spindle." in: The Journal of cell biology, Vol. 195, Issue 1, pp. 87-98, 2011 (PubMed).

Thompson, Compton: "Chromosome missegregation in human cells arises through specific types of kinetochore-microtubule attachment errors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 44, pp. 17974-8, 2011 (PubMed).

Serio, Margaria, Jensen, Oldani, Bartek, Bussolino, Lanzetti: "Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 42, pp. 17337-42, 2011 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Santaguida, Tighe, DAlise, Taylor, Musacchio: "Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine." in: The Journal of cell biology, Vol. 190, Issue 1, pp. 73-87, 2010 (PubMed). Further details: Immunocytochemistry,Immunofluorescence