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BUB1B antibody (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast)) (N-Term)

Details for Product anti-BUB1B Antibody No. ABIN151818, Supplier: Log in to see
Antigen
  • bub1b
  • BUB1
  • BUB1-like
  • BubR1
  • BubRI
  • CG14030
  • CG14030/Bub1
  • Dmel\\CG14030
  • bub1
  • 31/13
  • BUBR1
  • BcDNA:LD23835
  • Bub1
  • CG 7838
  • CG7838
  • DmBUB1
  • Dmbub1
  • Dmel\\CG7838
  • bubR1
  • bubr1
  • dBUB1
  • l(2)k03113
  • l(2)k06109
  • mcl(2)Z1525
  • BUB1beta
  • Bub1A
  • MAD3L
  • MVA1
  • SSK1
  • hBUBR1
  • AU045529
  • BUB1 mitotic checkpoint serine/threonine kinase B
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
  • Bub1 homologue
  • Bub1-related kinase
  • budding uninhibited by benzimidazoles 1 homolog, beta (S. cerevisiae)
  • bub1b
  • BUB1B
  • Bub1
  • BubR1
  • Bub1b
Epitope
N-Term, AA 1-350
46
26
16
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
169
72
13
9
2
2
Host
Mouse
96
73
6
Clonality (Clone)
Monoclonal ()
Conjugate
This BUB1B antibody is un-conjugated
5
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
150
72
58
45
34
31
27
14
13
5
4
3
3
3
2
2
1
Options
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Immunogen A portion of human BubR1 tagged at the N-terminus with pMAL (residues 1-350). [UniProt# O60566]
Clone 8G1
Isotype IgG1 kappa
Specificity This antibody is specific for hBUBR1. There should be no cross-reactivity with hBUB1.
Purification Protein G purified
Alternative Name BubR1 (BUB1B Antibody Abstract)
Background Gene Symbol: BUB1B
Molecular Weight Theoretical MW: 120 kDa
Gene ID 701
UniProt O60566
Research Area Kinases/Phosphatases, Cell Cycle, Cancer
Application Notes Western Blot 1:2000-1:5000, Flow Cytometry 1 μg per million cells, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, CyTOF-readyThis BubR1 (8G1) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western blot. By WB, this antibody recognizes a band at ~120 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-BUB1B antibody (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast)) (N-Term) (ABIN151818) BUBR1 detected in HeLa cell lysate using ABIN151818.
Western Blotting (WB) image for anti-BUB1B antibody (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast)) (N-Term) (ABIN151818) Detection of BUBR1 in HeLa WCE using ABIN151818.
Immunofluorescence (IF) image for anti-BUB1B antibody (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast)) (N-Term) (ABIN151818) anti-Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast) (BUB1B) (AA 1-350), (N-Term) antibody (Image 3)
Flow Cytometry (FACS) image for anti-BUB1B antibody (Budding Uninhibited By Benzimidazoles 1 Homolog beta (Yeast)) (N-Term) (ABIN151818) Flow Cytometry: BubR1 Antibody (8G1) [ABIN151818] - Intracellular flow cytometric sta...
Product cited in: Deeraksa, Pan, Sha, Liu, Eissa, Lin, Yu-Lee: "Plk1 is upregulated in androgen-insensitive prostate cancer cells and its inhibition leads to necroptosis." in: Oncogene, Vol. 32, Issue 24, pp. 2973-83, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Silió, Redondo-Muñoz, Carrera: "Phosphoinositide 3-kinase β regulates chromosome segregation in mitosis." in: Molecular biology of the cell, Vol. 23, Issue 23, pp. 4526-42, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

De Rycker, Rigoreau, Dowding, Parker: "A high-content, cell-based screen identifies micropolyin, a new inhibitor of microtubule dynamics." in: Chemical biology & drug design, Vol. 73, Issue 6, pp. 599-610, 2009 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Bohers, Sarafan-Vasseur, Drouet, Paresy, Latouche, Flaman, Sesboüé, Frebourg: "Gradual reduction of BUBR1 protein levels results in premature sister-chromatid separation then in aneuploidy." in: Human genetics, Vol. 124, Issue 5, pp. 473-8, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting

Trachana, van Wely, Guerrero, Fütterer, Martínez-A: "Dido disruption leads to centrosome amplification and mitotic checkpoint defects compromising chromosome stability." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 8, pp. 2691-6, 2007 (PubMed).

Chan, Jablonski, Sudakin, Hittle, Yen: "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC." in: The Journal of cell biology, Vol. 146, Issue 5, pp. 941-54, 1999 (PubMed).

Background publications Johnson, Scott, Holt, Hussein, Taylor: "Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression." in: Journal of cell science, Vol. 117, Issue Pt 8, pp. 1577-89, 2004 (PubMed).