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RPE65 antibody (Retinal Pigment Epithelium-Specific Protein 65kDa)

Details for Product anti-RPE65 Antibody No. ABIN151819, Supplier: Log in to see
Antigen
  • RPE56c
  • si:ch211-198n5.12
  • 65kDa
  • A930029L06Rik
  • LCA2
  • Mord1
  • RP20
  • rd12
  • mRPE65
  • sRPE65
  • retinal pigment epithelium-specific protein 65c
  • retinal pigment epithelium-specific protein 65kDa
  • retinal pigment epithelium 65
  • rpe65c
  • LOC100219959
  • LOC100352270
  • Rpe65
  • RPE65
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
52
50
44
31
21
20
18
18
2
2
1
1
Host
Mouse
27
26
10
1
Clonality (Clone)
Monoclonal ()
Conjugate
This RPE65 antibody is un-conjugated
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
46
23
21
18
16
16
13
12
5
2
1
Options
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Immunogen Bovine RPE65 microsomal membrane proteins. [UniProt# Q28175]
Clone 401-8B11-3D9
Isotype IgG1 kappa
Specificity This antibody is specific for RPE65.
Purification Protein G purified
Alternative Name RPE65 (RPE65 Antibody Abstract)
Background Gene Symbol: RPE65
Molecular Weight Theoretical MW: 65 kDa
Gene ID 6121
UniProt Q28175
Research Area Signaling, Metabolism, Neurology
Application Notes Western Blot 1:500 - 1:1000, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:250-1:500, Immunohistochemistry-Frozen 1:250-1:500, CyTOF-readyFor Western blot, this antibody has been validated in lysates of bovine RPE membrane and COS7 cells transfected with human RPE65, and in both samples, the antibody recognized a band at ~65 kDa, representing RPE65 protein. Note:- Hamel et al. have reported that in cultured bovine RPE cells, the levels of RPE65 are undetectable in WB after day 14, however, the mRNA levels are detectable by Northern for at least 7 weeks (PMID: 8340400). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for RPE65 Antibody Western Blot
1. Bovine RPE membrane (5ug) and recombinant human RPE65 (~20ug ) expressed in COS7 cells were loaded on a NuPAGE Novex Bis-Tris 10 %gel along with SeeBlue Plus2 marker.
. Run in NuPAGE MOPS electrophoresis buffer (Invitrogen). 3 Samples were probed with NB 100-355 RPE65 antibody (1:5,000 ) followed by alkaline phosphatase-conjugated goat anti-mouse IgG incubation.
. Visualized by NBT/BCIP substrate.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-RPE65 antibody (Retinal Pigment Epithelium-Specific Protein 65kDa) (ABIN151819) RPE65 detected in bovine and human samples. Lane 1: bovine RPE membrane, lane 2: reco...
Immunofluorescence (IF) image for anti-RPE65 antibody (Retinal Pigment Epithelium-Specific Protein 65kDa) (ABIN151819) Immunohistochemical staining of RPE65 in mouse retina tissue using ABIN151819.
Immunofluorescence (IF) image for anti-RPE65 antibody (Retinal Pigment Epithelium-Specific Protein 65kDa) (ABIN151819) Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) [ABIN151819] - ...
Product cited in: Inoue, Hasegawa, Yamada, Date, Mizutani, Nakata, Akamatsu: "Lignin Induces ES Cells to Differentiate into Neuroectodermal Cells through Mediation of the Wnt Signaling Pathway." in: PLoS ONE, Vol. 8, Issue 6, pp. e66376, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Cashman, Gracias, Adhi, Kumar-Singh: "Adenovirus-mediated delivery of Factor H attenuates complement C3 induced pathology in the murine retina: a potential gene therapy for age-related macular degeneration." in: The journal of gene medicine, Vol. 17, Issue 10-12, pp. 229-43, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Parker, Mitrousis, Shoichet: "Hydrogel for Simultaneous Tunable Growth Factor Delivery and Enhanced Viability of Encapsulated Cells in Vitro." in: Biomacromolecules, Vol. 17, Issue 2, pp. 476-84, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Chen, Rajapakse, Fraczek, Luo, Forrester, Xu: "Retinal pigment epithelial cell multinucleation in the aging eye - a mechanism to repair damage and maintain homoeostasis." in: Aging cell, Vol. 15, Issue 3, pp. 436-45, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Ayala-Peña, Pilotti, Volonté, Rotstein, Politi, German: "Protective effects of retinoid x receptors on retina pigment epithelium cells." in: Biochimica et biophysica acta, Vol. 1863, Issue 6 Pt A, pp. 1134-45, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Iwabe, Ying, Aguirre, Beltran: "Assessment of visual function and retinal structure following acute light exposure in the light sensitive T4R rhodopsin mutant dog." in: Experimental eye research, Vol. 146, pp. 341-53, 2016 (PubMed). (Sample species: Dog (Canine)). Further details: Immunohistochemistry (Frozen Sections)

Zhou, Rivera, Bhosle, Lahaie, Shao, Tahiri, Zhu, Polosa, Dorfman, Beaudry-Richard, Costantino, Lodygensky, Lachapelle, Chemtob: "Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1β." in: The American journal of pathology, Vol. 186, Issue 12, pp. 3100-3116, 2016 (PubMed). Method employed by authors: Flow Cytometry (FACS)

Di Lauro, Rodriguez-Crespo, Gayoso, Garcia-Gutierrez, Pastor, Srivastava, Fernandez-Bueno: "A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells." in: Molecular vision, Vol. 22, pp. 243-53, 2016 (PubMed).

Yan, He, Zhan, Wang: "Induction of ectopic retina-like tissue by transgenic expression of neurogenin." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116171, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Singh, Srivastava, Martín, Alonso, Pastor: "Bioactive substrates for human retinal pigment epithelial cell growth from elastin-like recombinamers." in: Journal of biomedical materials research. Part A, Vol. 102, Issue 3, pp. 639-46, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Prasad, Verma, Li: "Expression and cellular localization of the Mas receptor in the adult and developing mouse retina." in: Molecular vision, Vol. 20, pp. 1443-55, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Fernandez-Bueno, Rodriguez de la Rua, Hileeto, Parrado, Regueiro-Purriños, Sala-Puigdollers, Srivastava, Gonzalo-Orden, Pastor: "Histology and immunochemistry evaluation of autologous translocation of retinal pigment epithelium-choroid graft in porcine eyes." in: Acta ophthalmologica, Vol. 91, Issue 2, pp. e125-32, 2013 (PubMed). (Sample species: Pig (Porcine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Wallace, Moir, Malone, Duncan, Devarajan, Crane: "Regulation of T-lymphocyte CCL3 and CCL4 production by retinal pigment epithelial cells." in: Investigative ophthalmology & visual science, Vol. 54, Issue 1, pp. 722-30, 2013 (PubMed).

Shirasawa, Sonoda, Terasaki, Arimura, Otsuka, Yamashita, Uchino, Hisatomi, Ishibashi, Sakamoto: "TNF-α disrupts morphologic and functional barrier properties of polarized retinal pigment epithelium." in: Experimental eye research, Vol. 110, pp. 59-69, 2013 (PubMed). (Sample species: Pig (Porcine)). Further details: Western Blotting

Guziewicz, Zangerl, Komáromy, Iwabe, Chiodo, Boye, Hauswirth, Beltran, Aguirre: "Recombinant AAV-mediated BEST1 transfer to the retinal pigment epithelium: analysis of serotype-dependent retinal effects." in: PLoS ONE, Vol. 8, Issue 10, pp. e75666, 2013 (PubMed). (Sample species: Dog (Canine)). Further details: Immunohistochemistry (Frozen Sections)

Steinfeld, Steinfeld, Coronato, Hampel, Layer, Araki, Vogel-Höpker: "RPE specification in the chick is mediated by surface ectoderm-derived BMP and Wnt signalling." in: Development (Cambridge, England), Vol. 140, Issue 24, pp. 4959-69, 2013 (PubMed). (Sample species: Chicken). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Leung, Gvritishvili, Liu, Tombran-Tink: "ZIP2 and ZIP4 mediate age-related zinc fluxes across the retinal pigment epithelium." in: Journal of molecular neuroscience : MN, Vol. 46, Issue 1, pp. 122-37, 2012 (PubMed). Further details: Immunocytochemistry,Flow Cytometry,Immunofluorescence,Immunohistochemistry

Kleinman, Kaneko, Cho, Dridi, Fowler, Blandford, Albuquerque, Hirano, Terasaki, Kondo, Fujita, Ambati, Tarallo, Gelfand, Bogdanovich, Baffi, Ambati: "Short-interfering RNAs induce retinal degeneration via TLR3 and IRF3." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 20, Issue 1, pp. 101-8, 2012 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Krohne, Westenskow, Kurihara, Friedlander, Lehmann, Dorsey, Li, Zhu, Schultz, Wang, Siuzdak, Ding, Friedlander: "Generation of retinal pigment epithelial cells from small molecules and OCT4 reprogrammed human induced pluripotent stem cells." in: Stem cells translational medicine, Vol. 1, Issue 2, pp. 96-109, 2012 (PubMed).

Cho, Kim, Ku, Park, Lee, Yoo, Park, Song, Choi, Yu: "Generation of retinal pigment epithelial cells from human embryonic stem cell-derived spherical neural masses." in: Stem cell research, Vol. 9, Issue 2, pp. 101-9, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence