Solute Carrier Family 31, Member 1 (SLC31A1) (C-Term) antibody

Details for Product No. ABIN151866
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Antigen
Synonyms CRT, CT-R, CTR, CTR1, Slc31a1, Xctr1, ctr1, Ctr1, LRRGT00200, ctr-1, zgc:76839, 4930445G01Rik, AI787263, AU016967
Epitope
C-Term
(7), (1)
Reactivity
Human
(9)
Host
Rabbit
(9)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(5), (5), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mL
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Catalog No. ABIN151866
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Immunogen A synthetic peptide derived from a C-terminal sequence of human SLC31A1/CTR1
Purification affinity purified
Alternative Name CTR1
Background The trace metal copper (Cu) plays a crucial role in mammalian cells as a cofactor formany enzymes. Cu-related genetic diseases, such as Menkes disease and Wilsondisease, arise from a lack of Cu homeostasis in mammalian cells. CTR1 is ahigh-affinity copper-uptake protein. The C-terminal domain is similar to the Raf family ofprotein kinases, but it's first two-thirds encodes a novel protein domain. CTR1 providesthe primary avenue for copper uptake in mammalian cells, thereby, affecting Cuhomeostasis and embryonic development. Alternate Names: anti-copper transporter 1 antibody, anti-COPT1 antibody, anti-CTR1 antibody,anti-hCTR1 antibody, anti-solute carrier family 31 (copper transporters) member 1antibody, anti-SLC31A1 antibody. Related Diseases: Menkes disease and Wilson disease
Gene Symbol: SLC31A1
Gene ID 1317, 20529, 171135
UniProt O15431
Application Notes This SLC31A1/CTR1 antibody is useful for Immunocytochemistry/Immunofluorescence, and Immunohistochemistry-paraffin embedded sections..
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry 1:250, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin 1:250, Western Blot
Protocol Immunohistochemistry-paraffin protocol specific for SLC31A1 / CTR1 Antibody Immunohistochemistry Procedure
1. Cut 5 mm tissue sections and mount on a slide.
. Deparaffinize formalin-fixed and paraffin-embedded tissue sections with three 10 min soaks in xylene, and then rehydrate with two 5 min soaks in 100 % ethanol, two 5 minute soaks in 95 % ethanol and two 5 minute soaks in 70 % ethanol followed by a rinse (10 dips) in 1X PBS.
. Block endogenous peroxidases with 0.3 % H2O2 in PBS for 30 minutes followed by 3 rinses in 1X PBS.Perform antigen retrieval:
. Microwave in citrate buffer pH 5 for 5 minutes. Check buffer level and microwave again in citrate buffer pH 5 for another 5 minutes. Allow the slides to cool for 20 min and rinse 3X in 0.1 % Trition-X in PBS.
. Citrate buffer formula: 0.1M citric acid stock (21.01 g of citric acid in 1 L water) and 0.1M sodium citrate stock (29.41g of sodium citrate in 1 L water). Mix 9 mLs of citric acid stock with 41 mLs of sodium citrate stock and complete to 500 mL with distilled water.(Note: citrate buffer is now available for purchase from several companies including BD Pharmingen and Biogenx).Perform immunohistochemical staining according to the guidelines of the Catalyzed Signal Amplification System (DAKO, Carpinteria, CA, cat# K1500):
. Block non-specific protein binding by setting slides in chamber with 1 % BSA in PBS and incubating for 20 minutes.
. For amplification, incubate with CSA kit Protein block (solution #2) for 5 minutes.
. Incubate the slides with a 1:250 dilution of anti-hCTR1 antibody , diluted in 1 % BSA in PBS and incubate overnight at 4C.
. Wash slides with 3X TBS/0.1 % Tween.
. Apply Rabbit Link antibody (from DAKO, Carpinteria, CA, Cat # K1498 ) for 1 hour, followed by 3X wash with TBS/0.1 % Tween.
. Apply prepared streptavidin complex (from DAKO CSA kit, solutions #5,6,7) for 15 minutes, followed by 3X wash with TBS/0.1 % Tween.(Note: the prepared streptavidin complex needs to be made fresh and needs to sit for 30 minutes prior to use).
. Apply amplification reagent (from DAKO CSA kit, solution #8) for 15 minutes, followed by 3X wash with TBS/0.1 % Tween.
. Apply streptavidin peroxidase (from DAKO CSA kit, solution #9) for 15 minutes, followed by 3X wash with TBS/0.1 % Tween.
. Incubate with substrate: prepare fresh AEC (3-amino-9ethylcarbazol) (Vector Laboratories, Burlingame, California) and apply for 10-30 minutes followed by 3X wash with PBS.
. Counterstain with Mayers Hematoxylin for 2 minutes followed by 3X wash with water.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
anti-Solute Carrier Family 31, Member 1 (SLC31A1) (C-Term) antibody The photo on the left is antibody staining of hCTR1 overexpressing cell line. The photo on the right is antibody plus peptide competition staining.
anti-Solute Carrier Family 31, Member 1 (SLC31A1) (C-Term) antibody (2) Panel 1: human CTR1 staining of breast cancer tissue. Panel 2: human CTR1-antigen competition in breast cancer tissue.
General Holzer, Varki, Le et al.: "Expression of the human copper influx transporter 1 in normal and malignant human tissues." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 9, pp. 1041-9, 2006 (PubMed).

Peng, Lu, Janisse et al.: "PET of human prostate cancer xenografts in mice with increased uptake of 64CuCl2." in: Journal of nuclear medicine : official publication, Society of Nuclear Medicine, Vol. 47, Issue 10, pp. 1649-52, 2006 (PubMed).

Holzer, Howell: "The internalization and degradation of human copper transporter 1 following cisplatin exposure." in: Cancer research, Vol. 66, Issue 22, pp. 10944-52, 2006 (PubMed).

Jandial, Farshchi-Heydari, Larson et al.: "Enhanced delivery of cisplatin to intraperitoneal ovarian carcinomas mediated by the effects of bortezomib on the human copper transporter 1." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 2, pp. 553-60, 2009 (PubMed).

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