Add to Basket
|+1 404 474 4654|
|+1 888 205 9894 (TF)|
CAMP Responsive Element Binding Protein 1 (CREB1) (pSer121) antibody
|Synonyms||CREB, MGC9284, Creb, Creb-1, AV083133, 2310001E10Rik, 3526402H21Rik, JAL, hJAL, ATCE1, CREB3, CREB4, AIBZIP, CREB1, creb1, MGC52669, creb, MGC69285, MGC55598, zgc:55598, Creb1, DKFZp468J2311|
Alternatives Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Quantity||0.1 ml (0.3 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Gene ID||1385, 12912|
|Immunogen||A phospho-peptide spanning the Ser-121 phosphorylation site of human CREB.|
|Cross-Reactivity||Human, Mouse (Murine)|
|Description||Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zippertranscription factor that binds the cyclic AMP response element (CRE) and activatestranscription in response to a variety of extracellular signals including neurotransmitters,hormones, membrane depolarization, and growth and neurotrophic factors. CREB isalso regulated by DNA-damaging stimuli and is directly phosphorylated on Ser-121 bythe ataxia-telangiectasia-mutated protein kinase, ATM in response to ionizing radiation. Alternate Names: anti-Active transcription factor CREB antibody, anti-cAMP responsive element bindingprotein 1 antibody, anti-CREB1 antibody, anti-MGC9284 antibody, anti-Transactivatorprotein antibody.|
|Specificity||This antibody is specific for the Ser-121-phosphorylated form of CREB.Phosphorylated:Ser121. Species Reactivity: Reacts with human and mouse CREB. Other species have not been tested. Localization: Nuclear.|
|Application Notes||Suggested working dilutions: immunoprecipitation.5 ug,Western Blot 1:500|
|Purification||Aff - Purified|
|Buffer||Tris 50% glycerol. Preservative: 0.05% sodium azide.|
|Storage||Aliquot and store at -20C. Avoid freeze-thaw cycles.|
|Restrictions||For Research Use only|
|Detection of phospho-CREB in human HEK 293T cells. Top panel: probed with ABIN151873. Bottom panel: probed with CREB antibody. Lanes 2 and 4 treated with lambda phosphatase, lanes 3 and 4 treated with 0.2mM H202.|
Shi, Venkataraman, Dodson et al.: "Direct regulation of CREB transcriptional activity by ATM in response to genotoxic stress." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 16, pp. 5898-903, 2004 (PubMed).
Shi, Dodson, Shaikh et al.: "Ataxia-telangiectasia-mutated (ATM) is a T-antigen kinase that controls SV40 viral replication in vivo." in: The Journal of biological chemistry, Vol. 280, Issue 48, pp. 40195-200, 2005 (PubMed).
Dodson, Tibbetts: "DNA replication stress-induced phosphorylation of cyclic AMP response element-binding protein mediated by ATM." in: The Journal of biological chemistry, Vol. 281, Issue 3, pp. 1692-7, 2006 (PubMed).
Shanware, Trinh, Williams et al.: "Coregulated ataxia telangiectasia-mutated and casein kinase sites modulate cAMP-response element-binding protein-coactivator interactions in response to DNA damage." in: The Journal of biological chemistry, Vol. 282, Issue 9, pp. 6283-91, 2007 (PubMed).
Shi, Dodson, Mukhopadhyay et al.: "Identification of carboxyl-terminal MCM3 phosphorylation sites using polyreactive phosphospecific antibodies." in: The Journal of biological chemistry, Vol. 282, Issue 12, pp. 9236-43, 2007 (PubMed).