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Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN) (C-Term) antibody

Details for Product No. ABIN151887, Supplier: Log in to see
Antigen
  • 2310046M24Rik
  • A830014H24Rik
  • cb728
  • FIH1
  • HIF-1a
  • hif-1a
  • hif1a
  • HIF1a
  • id:ibd5139
Epitope
C-Term
36
25
20
3
3
2
2
2
2
2
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
172
71
49
5
3
2
2
1
Host
Rabbit
104
67
Clonality
Polyclonal
Conjugate
Un-conjugated
9
9
8
7
6
6
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
146
67
61
51
42
37
25
12
6
4
2
2
2
1
Supplier
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Immunogen A synthetic peptide made to the C-terminal region of human Factor Inhibiting HIF-1 (between residues 300 and the C-terminus). [UniProt# Q9NWT6]
Purification Immunogen affinity purified
Alternative Name FIH-1/HIF-1AN (HIF1AN Antibody Abstract)
Background Gene Symbol: HIF1AN
Molecular Weight Theoretical MW: 40.3 kDa
Gene ID 55662
UniProt Q9NWT6
Application Notes Western Blot 1:1000-1:5000, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Electron Microscopy
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for Factor Inhibiting HIF-1 Antibody
1. (30-40ug) of protein were separated via gel electrophoresis (10-20 %) under denaturing conditions and transferred to nitrocellulose membrane.
. Membrane was blocked overnight (4C) in TBS/Tween-20 (TBST) + 5 % milk (blocking buffer).
. Membrane was incubated with primary anti-FIH [cat# NB 100-428] (1:500) in blocking buffer for 2 hours at room temp.
. Membrane was washed 3 times with TBST. 10 minutes each wash.
. Membrane was incubated with HRP conjugated Donkey anti-rabbit secondary in blocking buffer for 2 hours at room temp.
. Membrane was washed 3 times with TBST. 10 minutes each wash.
. Membranes were developed for visualization using Super Signal(R) West Pico Chemiluminescent Substrate and captured on Hyperfilm ECL (Amersham Biosciences).Immunohistochemistry Protocol
. Prepared 18 micron frozen rat brain tissue sections (post fixed with 10 % formalin for 30 minutes).
. Dilute the primary antibody 1:100 and incubated with diluted FIH antibody (cat# NB 100-428) at 4C, overnight.
. Diluted the secondary antibody, biotinylated anti-rabbit IgG, according to the manufacturer's guidelines and incubated overnight at 4C.
. Diluted the tertiary antibody, HRP-conjugated streptavidin, according to the manufacturer's guidelines and incubated overnight at 4C.
. Visualized with DAB oxidation by HRP.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/phosphate, pH 7-8
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Immunohistochemistry (IHC) image for anti-Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN) (C-Term) antibody (ABIN151887) Immunohistochemistry: Factor Inhibiting HIF-1 Antibody [ABIN151887SS] - Detection of ...
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN) (C-Term) antibody (ABIN151887) Western Blot: Factor Inhibiting HIF-1 Antibody [ABIN151887SS] - Detection of FIH-1 in...
Product cited in: Nakaoka, Hara, Yoshino, Kanamori, Matsui, Shimamura, Sato, Murakami, Seiki, Sakamoto: "NECAB3 Promotes Activation of Hypoxia-inducible factor-1 during Normoxia and Enhances Tumourigenicity of Cancer Cells." in: Scientific reports, Vol. 6, pp. 22784, 2016 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Karttunen, Duffield, Scrimgeour, Squires, Lim, Dallas, Scragg, Chicher, Dave, Whitelaw, Peers, Gorman, Gleadle, Rychkov, Peet: "Oxygen-dependent hydroxylation by FIH regulates the TRPV3 ion channel." in: Journal of cell science, Vol. 128, Issue 2, pp. 225-31, 2015 (PubMed). Further details: Western Blotting

Kiriakidis, Henze, Kruszynska-Ziaja, Skobridis, Theodorou, Paleolog, Mazzone: "Factor-inhibiting HIF-1 (FIH-1) is required for human vascular endothelial cell survival." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 7, pp. 2814-27, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Rawluszko, Bujnicka, Horbacka, Krokowicz, Jagodziński: "Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer." in: BMC cancer, Vol. 13, pp. 526, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Janke, Brockmeier, Kuhlmann, Eisenacher, Nolde, Meyer, Mairbäurl, Metzen: "Factor inhibiting HIF-1 (FIH-1) modulates protein interactions of apoptosis-stimulating p53 binding protein 2 (ASPP2)." in: Journal of cell science, Vol. 126, Issue Pt 12, pp. 2629-40, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Wilkins, Karttunen, Hampton-Smith, Murchland, Chapman-Smith, Peet: "Factor inhibiting HIF (FIH) recognizes distinct molecular features within hypoxia-inducible factor-α (HIF-α) versus ankyrin repeat substrates." in: The Journal of biological chemistry, Vol. 287, Issue 12, pp. 8769-81, 2012 (PubMed).

Nishida, Kusubata, Tashiro, Gritli, Sato, Ohki-Koizumi, Morita, Nagano, Sakamoto, Koshikawa, Kuchimaru, Kizaka-Kondoh, Seiki, Nakauchi, Heissig, Hattori: "MT1-MMP plays a critical role in hematopoiesis by regulating HIF-mediated chemokine/cytokine gene transcription within niche cells." in: Blood, Vol. 119, Issue 23, pp. 5405-16, 2012 (PubMed).

Lim, Kiriakidis, Paleolog, Davies: "Increased activation of the hypoxia-inducible factor pathway in varicose veins." in: Journal of vascular surgery, Vol. 55, Issue 5, pp. 1427-39, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Singleton, Trudgian, Fischer, Kessler, Ratcliffe, Cockman: "Quantitative mass spectrometry reveals dynamics of factor-inhibiting hypoxia-inducible factor-catalyzed hydroxylation." in: The Journal of biological chemistry, Vol. 286, Issue 39, pp. 33784-94, 2011 (PubMed).

Melvin, Mudie, Rocha: "The chromatin remodeler ISWI regulates the cellular response to hypoxia: role of FIH." in: Molecular biology of the cell, Vol. 22, Issue 21, pp. 4171-81, 2011 (PubMed). (Sample species: Human).

Schödel, Bohr, Klanke, Schley, Schlötzer-Schrehardt, Warnecke, Kurtz, Amann, Eckardt, Willam: "Factor inhibiting HIF limits the expression of hypoxia-inducible genes in podocytes and distal tubular cells." in: Kidney international, Vol. 78, Issue 9, pp. 857-67, 2010 (PubMed).

Luo, Zhang, Nicholson, Zhu, McBride et al.: "Molecular characterization of antibody epitopes of Ehrlichia chaffeensis ankyrin protein 200 and tandem repeat protein 47 and evaluation of synthetic immunodeterminants for serodiagnosis of human ..." in: Clinical and vaccine immunology : CVI, Vol. 17, Issue 1, pp. 87-97, 2010 (PubMed).

Chan, Kawahara, Sutphin, Chang, Chi, Giaccia: "Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment." in: Cancer cell, Vol. 15, Issue 6, pp. 527-38, 2009 (PubMed).

Minamishima, Moslehi, Padera, Bronson, Liao, Kaelin: "A feedback loop involving the Phd3 prolyl hydroxylase tunes the mammalian hypoxic response in vivo." in: Molecular and cellular biology, Vol. 29, Issue 21, pp. 5729-41, 2009 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Zheng, Linke, Dias, Zheng, Gradin, Wallis, Hamilton, Gustafsson, Ruas, Wilkins, Bilton, Brismar, Whitelaw, Pereira, Gorman, Ericson, Peet, Lendahl, Poellinger: "Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3368-73, 2008 (PubMed). Further details: Western Blotting

Xu, Vaithiyalingam, Glick, Mordes, Chazin, Cortez: "The basic cleft of RPA70N binds multiple checkpoint proteins, including RAD9, to regulate ATR signaling." in: Molecular and cellular biology, Vol. 28, Issue 24, pp. 7345-53, 2008 (PubMed).

Li, Wang, Dutta, Lau, Jiang, Datta, Mukhopadhyay: "Protein kinase C-mediated modulation of FIH-1 expression by the homeodomain protein CDP/Cut/Cux." in: Molecular and cellular biology, Vol. 27, Issue 20, pp. 7345-53, 2007 (PubMed).

Birle, Hedley: "Suppression of the hypoxia-inducible factor-1 response in cervical carcinoma xenografts by proteasome inhibitors." in: Cancer research, Vol. 67, Issue 4, pp. 1735-43, 2007 (PubMed).

Ferguson, Wu, Smith, Charles, Powers, Wang, Patterson: "ASB4 is a hydroxylation substrate of FIH and promotes vascular differentiation via an oxygen-dependent mechanism." in: Molecular and cellular biology, Vol. 27, Issue 18, pp. 6407-19, 2007 (PubMed).

Khan, Michalopoulos, Stolz: "Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation." in: The American journal of pathology, Vol. 169, Issue 4, pp. 1251-69, 2006 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunoelectron Microscopy,Immunofluorescence