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Der1-Like Domain Family, Member 1 (DERL1) (AA 200-251), (C-Term) antibody

Details for Product No. ABIN151899, Supplier: Log in to see
Antigen
  • 1110021N07Rik
  • AI195141
  • AW551338
  • CG10908
  • DDBDRAFT_0188135
  • DDBDRAFT_0266772
  • DDB_0188135
  • DDB_0266772
  • DER-1
  • DER1
  • DERLIN-1
  • derlin-1
  • Derlin-1
  • Dmel\\CG10908
  • F17A13.150
  • F17A13_150
  • MGC84200
  • RGD1311835
  • wu:fb09d07
  • wu:fb94d05
  • wu:fj92g12
  • zgc:55637
Epitope
C-Term, AA 200-251
40
18
3
3
2
1
1
1
Reactivity
Chinese Hamster, Dog (Canine), Human, Mouse (Murine)
66
34
17
14
11
Host
Rabbit
63
3
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
49
26
19
15
12
7
7
5
2
2
1
Supplier
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Immunogen A synthetic peptide made to the C-terminal region of human Derlin 1 (between residues 200-251). [UniProt# Q9BUN8]
Cross-Reactivity (Details) Predicted to react with Xenopus, bovine, chicken, rat, zebrafish,mouse based on 100% sequence homology.
Purification Immunogen affinity purified
Alternative Name Derlin 1 (DERL1 Antibody Abstract)
Background Gene Symbol: DERL1
Molecular Weight Theoretical MW: 29 kDa
Gene ID 79139
UniProt Q9BUN8
Application Notes Western Blot 1:1000-1:5000, Immunohistochemistry 5-10 μg/mL, Immunocytochemistry/Immunofluorescence 1:2000, Immunohistochemistry-Paraffin 5-10 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Derlin 1 Antibody Western Blot
1. Run samples on a 10-15 % SDS-PAGE.
. Transfer proteins to a methanol soaked PVDF membrane for 2.5 hr at 250 milliamper at 4C on a stir plate.
. Immerse PVDF membrane in blocking buffer and block for 1 hour at room temperature.
. Wash membrane 3 times with TBS-T.
. Incubate with primary Derlin-1 antibody , diluted in blocking buffer 1:500, overnight at 4C
. Wash membrane 3 x 10 min with TBS-T.
. Incubate with secondary antibody, diluted in blocking buffer, at room temp for 1 hour.
. Wash 3 x 10 min with TBS-T.
. Detect with Amersham ECL kit (RPN 2106).
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/phosphate, pH 7-8
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-Der1-Like Domain Family, Member 1 (DERL1) (AA 200-251), (C-Term) antibody (ABIN151899) Detection of Derlin protein in canine KRM microsomes using ABIN151899.
Immunohistochemistry (IHC) image for anti-Der1-Like Domain Family, Member 1 (DERL1) (AA 200-251), (C-Term) antibody (ABIN151899) Staining of human liver using ABIN151899.
Immunofluorescence (IF) image for anti-Der1-Like Domain Family, Member 1 (DERL1) (AA 200-251), (C-Term) antibody (ABIN151899) Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [ABIN151899] - Derlin-1 ant...
Product cited in: Sugawara, Kano, Murata: "Rab2A is a pivotal switch protein that promotes either secretion or ER-associated degradation of (pro)insulin in insulin-secreting cells." in: Scientific reports, Vol. 4, pp. 6952, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Huttunen, Guénette, Peach, Greco, Xia, Kim, Barren, Tanzi, Kovacs: "HtrA2 regulates beta-amyloid precursor protein (APP) metabolism through endoplasmic reticulum-associated degradation." in: The Journal of biological chemistry, Vol. 282, Issue 38, pp. 28285-95, 2007 (PubMed). (Sample species: Chinese Hamster). Further details: Western Blotting