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Der1-Like Domain Family, Member 1 (DERL1) (AA 200-251), (C-Term) antibody

Details for Product No. ABIN151899, Supplier: Log in to see
Antigen
  • CG10908
  • Dmel\\CG10908
  • DDBDRAFT_0188135
  • DDBDRAFT_0266772
  • DDB_0188135
  • DDB_0266772
  • MGC84200
  • wu:fb09d07
  • wu:fb94d05
  • wu:fj92g12
  • zgc:55637
  • 1110021N07Rik
  • AI195141
  • AW551338
  • Derlin-1
  • derlin-1
  • DER-1
  • DER1
  • RGD1311835
  • DERLIN-1
  • F17A13.150
  • F17A13_150
  • Derlin-1
  • derlin-1
  • derlin-1.1
  • hypothetical protein
  • Der1-like domain family, member 1
  • derlin 1
  • Der-1
  • EDI_023440
  • PTRG_01331
  • derl1
  • DERL1
  • LOC100355027
  • Derl1
  • DER1
Epitope
C-Term, AA 200-251
38
18
3
2
2
2
2
1
1
1
Reactivity
Chinese Hamster, Dog (Canine), Human, Mouse (Murine)
67
43
18
16
11
1
1
1
1
Host
Rabbit
65
3
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
50
26
20
19
13
12
12
5
2
2
2
Options
Supplier
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Immunogen A synthetic peptide made to the C-terminal region of human Derlin 1 (between residues 200-251). [UniProt# Q9BUN8]
Purification Immunogen affinity purified
Alternative Name Derlin 1 (DERL1 Antibody Abstract)
Background Gene Symbol: DERL1
Molecular Weight Theoretical MW: 29 kDa
Gene ID 79139
UniProt Q9BUN8
Application Notes Western Blot 1:1000-1:5000, Immunohistochemistry 5-10 μg/mL, Immunocytochemistry/Immunofluorescence 1:2000, Immunohistochemistry-Paraffin 5-10 μg/mLThis Derlin 1 antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~29 kDa. In ICC/IF, ER staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Derlin 1 Antibody Western Blot
1. Run samples on a 10-15 % SDS-PAGE.
. Transfer proteins to a methanol soaked PVDF membrane for 2.5 hr at 250 milliamper at 4C on a stir plate.
. Immerse PVDF membrane in blocking buffer and block for 1 hour at room temperature.
. Wash membrane 3 times with TBS-T.
. Incubate with primary Derlin-1 antibody , diluted in blocking buffer 1:500, overnight at 4C
. Wash membrane 3 x 10 min with TBS-T.
. Incubate with secondary antibody, diluted in blocking buffer, at room temp for 1 hour.
. Wash 3 x 10 min with TBS-T.
. Detect with Amersham ECL kit (RPN 2106).
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-Der1-Like Domain Family, Member 1 (DERL1) (AA 200-251), (C-Term) antibody (ABIN151899) Detection of Derlin protein in canine KRM microsomes using ABIN151899.
Immunohistochemistry (IHC) image for anti-Der1-Like Domain Family, Member 1 (DERL1) (AA 200-251), (C-Term) antibody (ABIN151899) Staining of human liver using ABIN151899.
Immunofluorescence (IF) image for anti-Der1-Like Domain Family, Member 1 (DERL1) (AA 200-251), (C-Term) antibody (ABIN151899) Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [ABIN151899] - Derlin-1 ant...
Product cited in: Sugawara, Kano, Murata: "Rab2A is a pivotal switch protein that promotes either secretion or ER-associated degradation of (pro)insulin in insulin-secreting cells." in: Scientific reports, Vol. 4, pp. 6952, 2014 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Huttunen, Guénette, Peach, Greco, Xia, Kim, Barren, Tanzi, Kovacs: "HtrA2 regulates beta-amyloid precursor protein (APP) metabolism through endoplasmic reticulum-associated degradation." in: The Journal of biological chemistry, Vol. 282, Issue 38, pp. 28285-95, 2007 (PubMed). (Sample species: Chinese Hamster). Further details: Western Blotting