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Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody

Details for Product No. ABIN151900, Supplier: Log in to see
Antigen
  • hif1a
  • AA959795
  • HIF1alpha
  • MOP1
  • bHLHe78
  • HIF-1A
  • HIF-1alpha
  • HIF1
  • HIF1-ALPHA
  • PASD8
  • HIF1-alpha
  • hif-1a
  • hypoxia inducible factor 1 alpha
  • hypoxia inducible factor 1, alpha subunit
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • hypoxia-inducible factor 1 alpha
  • hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • Protein HIF-1
  • hif-1a
  • Hif1a
  • HIF1A
  • hif-1
Alternatives
anti-Human Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) antibody for Electrophoretic Mobility-Shift Assay
Epitope
C-Term
84
59
40
31
20
18
16
15
14
13
13
13
13
10
8
8
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Monkey, Mouse (Murine), Primate, Rat (Rattus)
610
296
249
145
92
76
66
63
58
54
43
37
28
21
19
19
18
16
16
7
3
2
2
2
1
1
1
1
1
Host
Rabbit
383
279
2
Clonality
Polyclonal
Conjugate
Un-conjugated
51
33
33
26
25
25
17
17
14
14
12
12
6
6
6
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Simple Western (SimWes), Western Blotting (WB)
518
228
201
184
170
169
106
90
61
15
15
13
13
13
10
7
6
4
3
3
3
2
2
2
1
1
Options
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Immunogen The immunogen recognized by this antibody maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1 (Q16665).
Isotype IgG
Purification Immunogen affinity purified
Alternative Name HIF-1 alpha (HIF1A Antibody Abstract)
Background Gene Symbol: HIF1A
Molecular Weight Theoretical MW: 93 kDa
Gene ID 3091
UniProt Q16665
Pathways Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis
Application Notes Western Blot 1:2000-1:10000, Simple Western 1:200, Chromatin Immunoprecipitation 1:10 - 1:500, Flow Cytometry 0.125 μg per 1 million cells in a 150 mcl reaction, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 2-5 μg/mg lysate, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Proximity Ligation Assay 1:200-1:4000, ImmunofluorescenceChIP usage was reported in scientific literature (PMID: 25557133). In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. For IHC-P, Tris-EDTA pH 9.0 buffer is recommended for the heat induced epitope retrieval. ELISA (PMID: 17556599 and 16966370).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer TBS and 0.1 % BSA
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Detection of mouse HIF1-alpha on hypoxia treated MEFs using ABIN151900.
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (Image 2)
Immunohistochemistry (IHC) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Mouse Brain, Neurons 40X
Simple Western (SimWes) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Simple Western: HIF-1 alpha Antibody [ABIN188610] - Simple Western lane view shows a ...
Flow Cytometry (FACS) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Flow Cytometry: HIF-1 alpha Antibody [ABIN188610] - Hela cells were treated for 15 hr...
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Western Blot: HIF-1 alpha Antibody [ABIN188610] - HIF-1 alpha induction on Caki-1 cel...
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Western Blot: HIF-1 alpha Antibody [ABIN188610] - Analysis of HIF-1 alpha in human my...
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Western Blot: HIF-1 alpha Antibody [ABIN188610] - Homogenate from pig (lanes 1 and 2)...
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Western Blot: HIF-1 alpha Antibody [ABIN188610] - Detection of Human HIF1 alpha by We...
Immunoprecipitation (IP) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Immunoprecipitation: HIF-1 alpha Antibody [ABIN188610] - Analysis in HEK293 cells. Im...
Immunofluorescence (IF) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [ABIN188610] - Formaldeh...
Proximity Ligation Assay (PLA) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Proximity Ligation Assay: HIF-1 alpha Antibody [ABIN188610] - Shows representative re...
Immunofluorescence (IF) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Immunofluorescence: HIF-1 alpha Antibody [ABIN188610] - Murine primary bone marrow de...
Proximity Ligation Assay (PLA) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Proximity Ligation Assay: HIF-1 alpha Antibody [ABIN188610] - Secondary-conjugate Duo...
Proximity Ligation Assay (PLA) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Proximity Ligation Assay: HIF-1 alpha Antibody [ABIN188610] - Secondary-conjugate Duo...
Proximity Ligation Assay (PLA) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (C-Term) antibody (ABIN151900) Proximity Ligation Assay: HIF-1 alpha Antibody [ABIN188610] - Secondary-conjugate Duo...
Product cited in: Lojk, Mis, Pirkmajer, Pavlin: "siRNA delivery into cultured primary human myoblasts--optimization of electroporation parameters and theoretical analysis." in: Bioelectromagnetics, Vol. 36, Issue 8, pp. 551-63, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Shum, White, Mills, Bentley, Eliseev: "Energy Metabolism in Mesenchymal Stem Cells During Osteogenic Differentiation." in: Stem cells and development, Vol. 25, Issue 2, pp. 114-22, 2016 (PubMed).

Chapple, Keeley, Mastronicola, Arno, Vizcay-Barrena, Fleck, Siow, Mann: "Bach1 differentially regulates distinct Nrf2-dependent genes in human venous and coronary artery endothelial cells adapted to physiological oxygen levels." in: Free radical biology & medicine, Vol. 92, pp. 152-62, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Shukla, Chaudhry, Mir, Gangwar, Yadav, Manda, Meena, Rao: "Chronic ethanol feeding promotes azoxymethane and dextran sulfate sodium-induced colonic tumorigenesis potentially by enhancing mucosal inflammation." in: BMC cancer, Vol. 16, pp. 189, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Kobayashi, Liu, Binns, Urrutia, Davidoff, Kapitsinou, Pfaff, Olauson, Wernerson, Fogo, Fong, Gross, Haase: "Distinct subpopulations of FOXD1 stroma-derived cells regulate renal erythropoietin." in: The Journal of clinical investigation, Vol. 126, Issue 5, pp. 1926-38, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Mirzamohammadi, Papaioannou, Inloes, Rankin, Xie, Schipani, Orkin, Kobayashi: "Polycomb repressive complex 2 regulates skeletal growth by suppressing Wnt and TGF-β signalling." in: Nature communications, Vol. 7, pp. 12047, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Semba, Takeda, Isagawa, Sugiura, Honda, Wake, Miyazawa, Yamaguchi, Miura, Jenkins, Choi, Kim, Asagiri, Cowburn, Abe, Soma, Koyama, Katoh, Sayama, Goda, Johnson, Manabe, Nagai, Komuro: "HIF-1α-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity." in: Nature communications, Vol. 7, pp. 11635, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Prakash, Nadella, Singh, Schmitz-Winnenthal: "CD14/TLR4 priming potentially recalibrates and exerts anti-tumor efficacy in tumor associated macrophages in a mouse model of pancreatic carcinoma." in: Scientific reports, Vol. 6, pp. 31490, 2016 (PubMed).

Mills, Kelly, Logan, Costa, Varma, Bryant, Tourlomousis, Däbritz, Gottlieb, Latorre, Corr, McManus, Ryan, Jacobs, Szibor, Xavier, Braun, Frezza, Murphy, ONeill: "Succinate Dehydrogenase Supports Metabolic Repurposing of Mitochondria to Drive Inflammatory Macrophages." in: Cell, Vol. 167, Issue 2, pp. 457-470.e13, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Nüsken, Wohlfarth, Lippach, Rauh, Schneider, Dötsch, Nüsken: "Reduced Perinatal Leptin Availability May Contribute to Adverse Metabolic Programming in a Rat Model of Uteroplacental Insufficiency." in: Endocrinology, Vol. 157, Issue 5, pp. 1813-25, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Farsijani, Liu, Kobayashi, Davidoff, Sha, Fandrey, Ikizler, OConnor, Haase: "Renal epithelium regulates erythropoiesis via HIF-dependent suppression of erythropoietin." in: The Journal of clinical investigation, Vol. 126, Issue 4, pp. 1425-37, 2016 (PubMed).

Turlejski, Humoud, Desai, Smith, Marina: "Immunohistochemical evidence of tissue hypoxia and astrogliosis in the rostral ventrolateral medulla of spontaneously hypertensive rats." in: Brain research, Vol. 1650, pp. 178-183, 2016 (PubMed).

McGettrick, Corcoran, Barry, McFarland, Crès, Curtis, Franklin, Corr, Mok, Cummins, Taylor, ONeill, Nolan: "Trypanosoma brucei metabolite indolepyruvate decreases HIF-1α and glycolysis in macrophages as a mechanism of innate immune evasion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 48, pp. E7778-E7787, 2016 (PubMed). Method employed by authors: Immunocytochemistry (ICC), Immunofluorescence (IF) (Sample species: Human).

Wang, Wang, Li, Entenberg, Xue, Wang, Condeelis: "Direct visualization of the phenotype of hypoxic tumor cells at single cell resolution in vivo using a new hypoxia probe." in: Intravital, Vol. 5, Issue 2, 2016 (PubMed). (Sample species: Human).

Appel, Turnwald, Ankerne, Wohlfarth, Appel, Rother, Janoschek, Alejandre Alcazar, Schnare, Meißner, Dötsch: "Hypoxia-Mediated Soluble Fms-Like Tyrosine Kinase 1 Increase Is Not Attenuated in Interleukin 6-Deficient Mice." in: Reproductive sciences (Thousand Oaks, Calif.), Vol. 22, Issue 6, pp. 735-42, 2015 (PubMed).

Palsson-McDermott, Curtis, Goel, Lauterbach, Sheedy, Gleeson, van den Bosch, Quinn, Domingo-Fernandez, Johnston, Jiang, Jiang, Israelsen, Keane, Thomas, Clish, Vander Heiden, Vanden Heiden, Xavier et al.: "Pyruvate kinase M2 regulates Hif-1α activity and IL-1β induction and is a critical determinant of the warburg effect in LPS-activated macrophages. ..." in: Cell metabolism, Vol. 21, Issue 1, pp. 65-80, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Chang, Paterno, Duscher, Maan, Chen, Januszyk, Rodrigues, Rennert, Bishop, Whitmore, Whittam, Longaker, Gurtner: "Exercise induces stromal cell-derived factor-1α-mediated release of endothelial progenitor cells with increased vasculogenic function." in: Plastic and reconstructive surgery, Vol. 135, Issue 2, pp. 340e-50e, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Robin, Marcillac, Raddatz: "A hypoxic episode during cardiogenesis downregulates the adenosinergic system and alters the myocardial anoxic tolerance." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 308, Issue 7, pp. R614-26, 2015 (PubMed). (Sample species: Chicken). Further details: Western Blotting

Nüsken, Herrmann, Wohlfarth, Goecke, Appel, Schneider, Dötsch, Nüsken: "Strong hypoxia reduces leptin synthesis in purified primary human trophoblasts." in: Placenta, Vol. 36, Issue 4, pp. 427-32, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Teranishi, Matsubara, Krausz, Le, Gonzalez, Yoshizato, Ikeda, Kawada: "Involvement of hepatic stellate cell cytoglobin in acute hepatocyte damage through the regulation of CYP2E1-mediated xenobiotic metabolism." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 95, Issue 5, pp. 515-24, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting