MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody

Antigen: MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A)

Synonyms: ATLD, HNGS1, MRE11, MRE11B, Mre11, zgc:64018, MRE11A

Clonality: Monoclonal (12D7)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN151920

Additional Information

Alternative name: MRE11

Gene ID: 4361

Immunogen: Amino acids 182-582 of Mre11 expressed in e. coli.

Cross-Reactivity: Human

Isotype: IgG1

Clone: 12D7

Description: Mre11 is an 80 kDa protein that acts as a Mn+2 dependent double stranded DNA 3'-5'exonuclease and a single stranded DNA endonuclease. It is part of a larger complexwith Rad50, p95 (nibrin) and possibly other associated proteins involved in the nuclelyticend-processing of double strand breaks and telomere maintenance. Alternate Names: anti-Ataxia-telangiectasia disorder-like antibody, anti-ATLD antibody, anti-MRE11aantibody, anti-MRE11b antibody.

Specificity: Species Reactivity: Cross-reacts with Human. Not yet tested in other species.

Application Details

Application Notes: Suggested working dilutions: immunofluorescence 1:100,immunoprecipitation 1-5 ug/ml,Western Blot 1-5 ug/ml,Immunohistochemistry-Paraffin 1:100. Positive Controls: T24 cells

Concentration: 1 mg/ml

Purification: Purified

Buffer: Phosphate buffered saline. Preservative: no preservative.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

anti-MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody

anti-MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody (Image 2)

Publications

Carney, Maser, Olivares et al.: "The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response." in: Cell, Vol. 93, Issue 3, pp. 477-86, 1998 (PubMed).

Trujillo, Yuan, Lee et al.: "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95." in: The Journal of biological chemistry, Vol. 273, Issue 34, pp. 21447-50, 1998 (PubMed).

Moreau, Ferguson, Symington: "The nuclease activity of Mre11 is required for meiosis but not for mating type switching, end joining, or telomere maintenance." in: Molecular and cellular biology, Vol. 19, Issue 1, pp. 556-66, 1999 (PubMed).

Zhong, Chen, Li et al.: "Association of BRCA1 with the hRad50-hMre11-p95 complex and the DNA damage response." in: Science (New York, N.Y.), Vol. 285, Issue 5428, pp. 747-50, 1999 (PubMed).

Limoli, Giedzinski, Bonner et al.: "UV-induced replication arrest in the xeroderma pigmentosum variant leads to DNA double-strand breaks, gamma -H2AX formation, and Mre11 relocalization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 1, pp. 233-8, 2002 (PubMed).

Giannini, Rinaldi, Ristori et al.: "Mutations of an intronic repeat induce impaired MRE11 expression in primary human cancer with microsatellite instability." in: Oncogene, Vol. 23, Issue 15, pp. 2640-7, 2004 (PubMed).

Ottini, Falchetti, Saieva et al.: "MRE11 expression is impaired in gastric cancer with microsatellite instability." in: Carcinogenesis, Vol. 25, Issue 12, pp. 2337-43, 2004 (PubMed).

Helmink, Bredemeyer, Lee et al.: "MRN complex function in the repair of chromosomal Rag-mediated DNA double-strand breaks." in: The Journal of experimental medicine, Vol. 206, Issue 3, pp. 669-79, 2009 (PubMed).

Zheng, Kanagaraj, Mihaljevic et al.: "MRE11 complex links RECQ5 helicase to sites of DNA damage." in: Nucleic acids research, Vol. 37, Issue 8, pp. 2645-57, 2009 (PubMed).