anti-PINK1 antibody (PTEN Induced Putative Kinase 1) (N-Term)

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N-Term, AA 1-50
(38), (34), (21), (15), (13), (13), (6), (6), (6), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(130), (79), (44), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(109), (44), (16)
This PINK1 antibody is un-conjugated
(10), (8), (7), (7), (7), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(146), (67), (45), (26), (23), (21), (4), (3)
Pubmed 2 references available
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Quantity 0.1 mL
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Immunogen An N-terminal region synthetic peptide made to the human PINK1 protein sequence (between residues 1-50).
Cross-Reactivity (Details) rat,reactivity reported in scientific literature (PMID: 24411077)
Purification affinity purified
Alternative Name PINK1 (PINK1 Antibody Abstract)
Background Phosphatase and Tensin Homolog (PTEN) dephosphorylates lipids. Malfunctions in thiscontribute to many human cancers. PTEN induced putative kinase 1 (Pink1) was firstdiscovered when researching the PTEN signaling pathway, thus Pink1 is also involvedin many human cancers. It is found in the mitochondria and its homozygousC-terminus mutation has been implicated in the early development of Parkinson'sdisease. Alternate Names: anti-Pink1 antibody, anti-PARK6 antibody, anti-BRPK antibody, anti-PTEN-inducedputative kinase protein 1 antibody, anti-Serine/threonin-protein kinase PINK1mitochondria [precursor] antibody.
Gene Symbol: PINK1
Gene ID 65018, 68943
UniProt Q9BXM7
Research Area Signaling, Metabolism
Application Notes This PINK1 antibody is useful for Western blot, where a band is seen ~60 kDa.
Recommended dilutions: Western Blot 1:500
Protocol Protocol specific for PINK1 Antibody Western Blot
1. Load 10-30 µg of whole cell lysate and mitochondrial fractions* on an 18 % Criterion Polyacrylamide Tris-HCl gel (BioRad).
. Transfer the proteins at 90V for 2 hours at 4 °C.
. Block the membrane in 5 % NFDM + TBSt for 1 hour at room temperature.
. Dilute the PINK1 primary antibody 1:500 in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane for 1 hour in TBST, changing the wash buffer every 5-10 minutes.
. Dilute the secondary antibody (as per manufacturer's guidelines) in TBST + 1 % BSA and incubate for 1 hour at room temperature.
. Develop the blot using ECL Plus.*NOTE: Mitochondria were isolated using Pierce's mitochondria isolation kit (#89874). The protocol for this kit procedure is online at Pierce.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-PINK1 antibody (PTEN Induced Putative Kinase 1) (N-Term) (ABIN151937) anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 1-50), (N-Term) antibody
Product cited in: Exner, Treske, Paquet et al.: "Loss-of-function of human PINK1 results in mitochondrial pathology and can be rescued by parkin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 45, pp. 12413-8, 2007 (PubMed).

Meijer, Karimi-Busheri, Huang et al.: "Pnk1, a DNA kinase/phosphatase required for normal response to DNA damage by gamma-radiation or camptothecin in Schizosaccharomyces pombe." in: The Journal of biological chemistry, Vol. 277, Issue 6, pp. 4050-5, 2002 (PubMed).