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PINK1 antibody (PTEN Induced Putative Kinase 1) (N-Term)

Details for Product anti-PINK1 Antibody No. ABIN151937, Supplier: Log in to see
Antigen
  • 1190006F07Rik
  • AU042772
  • AW557854
  • BEST:GH23468
  • BRPK
  • CG4523
  • Dmel\\CG4523
  • dPINK1
  • dPink1
  • mFLJ00387
  • PARK6
  • PINK
  • pink1
  • PINK1
  • wu:fc39e12
  • zgc:101729
Epitope
N-Term, AA 1-50
45
38
32
21
19
19
17
13
13
6
5
5
5
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
179
131
74
2
1
1
1
1
1
1
1
1
Host
Rabbit
154
48
15
Clonality
Polyclonal
Conjugate
This PINK1 antibody is un-conjugated
13
12
10
7
7
7
5
5
5
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
174
89
71
61
59
56
3
3
2
2
Supplier
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Immunogen An N-terminal region synthetic peptide made to the human PINK1 protein sequence (between residues 1-50). [UniProt# Q9BXM7]
Cross-Reactivity (Details) rat,reactivity reported in scientific literature (PMID: 24411077)
Purification Immunogen affinity purified
Alternative Name PINK1 (PINK1 Antibody Abstract)
Background Gene Symbol: PINK1
Molecular Weight Theoretical MW: 63 kDa
Gene ID 65018, 68943
UniProt Q9BXM7
Research Area Signaling, Metabolism
Application Notes Western Blot 1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for PINK1 Antibody Western Blot
1. Load 10-30 µg of whole cell lysate and mitochondrial fractions* on an 18 % Criterion Polyacrylamide Tris-HCl gel (BioRad).
. Transfer the proteins at 90V for 2 hours at 4 °C.
. Block the membrane in 5 % NFDM + TBSt for 1 hour at room temperature.
. Dilute the PINK1 primary antibody 1:500 in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane for 1 hour in TBST, changing the wash buffer every 5-10 minutes.
. Dilute the secondary antibody (as per manufacturer's guidelines) in TBST + 1 % BSA and incubate for 1 hour at room temperature.
. Develop the blot using ECL Plus.*NOTE: Mitochondria were isolated using Pierce's mitochondria isolation kit (#89874). The protocol for this kit procedure is online at Pierce.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/phosphate, pH 7-8
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-PINK1 antibody (PTEN Induced Putative Kinase 1) (N-Term) (ABIN151937) anti-PTEN Induced Putative Kinase 1 (PINK1) (AA 1-50), (N-Term) antibody
Product cited in: Amadoro, Corsetti, Florenzano, Atlante, Ciotti, Mongiardi, Bussani, Nicolin, Nori, Campanella, Calissano: "AD-linked, toxic NH2 human tau affects the quality control of mitochondria in neurons." in: Neurobiology of disease, Vol. 62, pp. 489-507, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Yasuda, Hayakawa, Nihira, Ren, Nakata, Nagai, Hattori, Miyake, Takada, Shimada, Mizuno, Mochizuki: "Parkin-mediated protection of dopaminergic neurons in a chronic MPTP-minipump mouse model of Parkinson disease." in: Journal of neuropathology and experimental neurology, Vol. 70, Issue 8, pp. 686-97, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Lutz, Exner, Fett, Schlehe, Kloos, Lämmermann, Brunner, Kurz-Drexler, Vogel, Reichert, Bouman, Vogt-Weisenhorn, Wurst, Tatzelt, Haass, Winklhofer: "Loss of parkin or PINK1 function increases Drp1-dependent mitochondrial fragmentation." in: The Journal of biological chemistry, Vol. 284, Issue 34, pp. 22938-51, 2009 (PubMed).

Exner, Treske, Paquet, Holmström, Schiesling, Gispert, Carballo-Carbajal, Berg, Hoepken, Gasser, Krüger, Winklhofer, Vogel, Reichert, Auburger, Kahle, Schmid, Haass: "Loss-of-function of human PINK1 results in mitochondrial pathology and can be rescued by parkin." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 45, pp. 12413-8, 2007 (PubMed). (Sample species: Human). Further details: Western Blotting

Meijer, Karimi-Busheri, Huang, Weinfeld, Young: "Pnk1, a DNA kinase/phosphatase required for normal response to DNA damage by gamma-radiation or camptothecin in Schizosaccharomyces pombe." in: The Journal of biological chemistry, Vol. 277, Issue 6, pp. 4050-5, 2002 (PubMed).