p300 antibody (E1A Binding Protein P300) (AA 1572-2371)

Details for Product anti-EP300 Antibody No. ABIN151948, Supplier: Log in to see
Antigen
  • KAT3B
  • RSTS2
  • p300
  • A430090G16
  • A730011L11
  • p300 HAT
  • E1A binding protein p300
  • E1A-associated protein p300
  • EP300
  • ep300
  • Ep300
Alternatives
anti-Rat (Rattus) p300 antibody for Chromatin Immunoprecipitation
Epitope
AA 1572-2371
56
52
37
18
15
11
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Mustelid, Primate, Rat (Rattus)
265
126
115
38
36
15
3
3
3
3
3
Host
Mouse
161
111
Clonality (Clone)
Monoclonal ()
Conjugate
This p300 antibody is un-conjugated
12
12
10
8
6
6
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
185
96
83
71
54
51
49
48
28
10
5
2
2
2
1
1
1
1
1
1
Options
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Immunogen Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
Clone RW128
Isotype IgG1 kappa
Purification Protein A purified
Alternative Name KAT3B/p300 (EP300 Antibody Abstract)
Background Gene Symbol: EP300
Molecular Weight Theoretical MW: 300 kDa
Gene ID 2033
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size
Application Notes Western Blot 1:250-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 1:10-1:500, Flow (Intracellular) 1 μg/mLThis KAT3B/p300 (RW128) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot, where a band is seen at ~300 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for KAT3B / p300 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 % Tris-acetate) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1.5 hours.
. Dilute the mouse anti-p300 primary antibody in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-p300 antibody (E1A Binding Protein P300) (AA 1572-2371) (ABIN151948) Detection of p300 in a HeLa nuclear extract using ABIN151948 (1:250). ECL: 15 second...
Product cited in: Nemethova, Wintersberger: "Polyomavirus large T antigen binds the transcriptional coactivator protein p300." in: Journal of virology, Vol. 73, Issue 2, pp. 1734-9, 1999 (PubMed).

Bhattacharya, Michels, Leung, Arany, Kung, Livingston: "Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1." in: Genes & development, Vol. 13, Issue 1, pp. 64-75, 1999 (PubMed).

Eckner, Ludlow, Lill, Oldread, Arany, Modjtahedi, DeCaprio, Livingston, Morgan: "Association of p300 and CBP with simian virus 40 large T antigen." in: Molecular and cellular biology, Vol. 16, Issue 7, pp. 3454-64, 1996 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunoprecipitation

Eckner, Yao, Oldread, Livingston: "Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation." in: Genes & development, Vol. 10, Issue 19, pp. 2478-90, 1996 (PubMed).

Eckner, Ewen, Newsome, Gerdes, DeCaprio, Lawrence, Livingston: "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor." in: Genes & development, Vol. 8, Issue 8, pp. 869-84, 1994 (PubMed).