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VEGFR1/Flt-1 (AA 800-900), (Internal Region) antibody

Details for Product No. ABIN151960, Supplier: Log in to see
Antigen
Epitope
Internal Region, AA 800-900
20
10
10
7
5
2
1
Reactivity
Human
82
20
19
Host
Rabbit
74
21
12
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
6
6
5
4
4
4
4
4
4
4
4
4
3
3
3
3
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
82
55
21
21
13
13
4
2
2
1
1
Supplier
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Immunogen A synthetic peptide made to an internal region of the mouse VEGF Receptor 1 protein (between residues 800-900) [UniProt# P35969]. There is 87% identity between the immunogen used for this production and the VEGF Receptor 2 protein.
Specificity This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.
Purification Immunogen affinity purified
Background Gene Symbol: FLT1
UniProt P35969
Application Notes Western Blot 1:100-1:2000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry-Paraffin
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for VEGF Receptor 1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 50 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 0.5 % BSA in TBS for 1 hour.
. Dilute the rabbit anti-VEGFR1 primary antibody in blocking buffer and incubate 2 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-glycine, 150 mM NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-VEGFR1/Flt-1 (AA 800-900), (Internal Region) antibody (ABIN151960) Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.
Immunofluorescence (IF) image for anti-VEGFR1/Flt-1 (AA 800-900), (Internal Region) antibody (ABIN151960) Immunocytochemistry/Immunofluorescence: VEGF R1/Flt-1 Antibody [ABIN151960] - VEGF R1...
Product cited in: Puglisi, Puppin, Pegolo, Andreetta, Pascoletti, DAurizio, Pandolfi, Fasola, Piga, Damante, Di Loreto: "Expression of periostin in human breast cancer." in: Journal of clinical pathology, Vol. 61, Issue 4, pp. 494-8, 2008 (PubMed).

Ptaszynska, Pendrak, Bandle, Stracke, Roberts: "Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells." in: Molecular cancer research : MCR, Vol. 6, Issue 3, pp. 352-63, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting

Takahashi, Hattori, Iwamatsu, Takizawa, Shibuya: "A novel snake venom vascular endothelial growth factor (VEGF) predominantly induces vascular permeability through preferential signaling via VEGF receptor-1." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46304-14, 2004 (PubMed).

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000 (PubMed).

Background publications Gluzman-Poltorak, Cohen, Shibuya, Neufeld: "Vascular endothelial growth factor receptor-1 and neuropilin-2 form complexes." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 18688-94, 2001 (PubMed).