C-C Chemokine Receptor Type 5 (CCR5) antibody

Details for Product No. ABIN151963
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Antigen
Synonyms AM4-7, CD195, Cmkbr5, Ckr5, CC-CKR-5, CCR-5, CMKBR5, GPCR, CCR5, CCCKR5, CKR-5, CKR5, IDDM22
Reactivity
Human, Monkey
(212), (69), (41), (1), (1)
Host
Rabbit
(135), (43), (42), (20), (6), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(23), (17), (12), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunofluorescence (IF), In vitro Assay (in vitro)
(132), (105), (78), (29), (28), (15), (15), (11), (10), (5), (4), (4), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 0.1 mL
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Catalog No. ABIN151963
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Immunogen A synthetic peptide demonstrating a high affinity towards the monoclonal CCR5 2D7. This peptide is known as the 2D7-2SK peptide and consists of the following residues: EWQKEGLVTLWLGGGGSC.
Specificity This antibody is specific for the linear epitope detected by the monoclonal antibody 2D7.
Purification Immunogen affinity purified
Alternative Name CD195 / CCR5
Background CCR5 is a major coreceptor for human HIV infection. Alternate Names: anti-CCR5 antibody, anti-CCR 5 antibody, anti-C-C CKR-5 antibody, anti-CC-CKR-5antibody, anti-HIV-1 fusion coreceptor antibody, anti-CHEMR13 antibody, anti-CD195antigen antibody, anti-CMKBR5 antibody.
Gene Symbol: CCR5
Gene ID 1234
Research Area CD Antigens, Surface Receptors of Immune Cells, Chemokines, Receptors
Application Notes This CCR5 antibody may be used for Western blot, ELISA, Immunocytochemistry/Immunofluorescence and Flow Cytometry, as well as being useful for inhibition of CCR5 tropic HIV envelope mediated fusion, syncytia formation, and infection in various in vitro assays.
Recommended dilutions: ELISA 1 µg/mL, Flow Cytometry 25 µg/mL, Functional, Immunocytochemistry/Immunofluorescence 1:100, In vitro assay, Western Blot 1.0 µg/mL
Protocol Flow Cytometry protocol specific for CCR5 Antibody Flow Cytometry
1. CEM.NKR.CCR5 cells were stained with 25 µg/ml anti-CCR5 or with preimmune rabbit IgG for 60 minutes on ice.
. This was followed by extensive washing and staining with a FITC-conjugated goat anti-rabbit secondary antibody.
. Flow cytometry was performed and analyzed with Cell Quest Software on FACSCalibur (BD Biosciences).Western blot protocol specific for CCR5 Antibody Western Blot Protocol
. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Trkola, Matthews, Gordon et al.: "A cell line-based neutralization assay for primary human immunodeficiency virus type 1 isolates that use either the CCR5 or the CXCR4 coreceptor." in: Journal of virology, Vol. 73, Issue 11, pp. 8966-74, 1999 (PubMed).

Khurana, Kennedy, King et al.: "Identification of a linear peptide recognized by monoclonal antibody 2D7 capable of generating CCR5-specific antibodies with human immunodeficiency virus-neutralizing activity." in: Journal of virology, Vol. 79, Issue 11, pp. 6791-800, 2005 (PubMed).

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