Wings Apart-Like Protein Homolog (WAPAL) antibody

Antigen: Wings Apart-Like Protein Homolog (WAPAL)

Synonyms: FOE, WAPL, KIAA0261

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine)

Application: Western Blotting (WB)

Catalog no.: ABIN151984

Additional Information

Alternative name: WAPAL

Gene ID: 23063

Swiss-Prot: Q7Z5K2

Immunogen: The epitope recognized by this antibody maps to a region between residues 1125 and1175 of human wings apart like/KIAA0261 using the numbering given in TrEMBL entryQ7Z5K2 (GeneID 23063).

Description: The hWAPL mRNA was predominantly expressed in uterine cervical cancer, with weakexpression in all other normal and tumor tissues examined. hWAPL expression inbenign epithelia was confined to the basal cell layers, whereas in dysplasias itincreasingly appeared in more superficial cell layers and showed a significant correlationwith severity of dysplasia. Diffuse hWAPL expression was found in all invasivesquamous cell carcinomas examined. These results demonstrate that hWAPL isassociated with cell growth, and the hWAPL expression may play a significant role incervical carcinogenesis and tumor progression. Alternate Names: anti-WAPL antibody, anti-hWAPL antibody, anti-human homolog of wings apart likeprotein antibody, anti-FOEfriend of EBNA2 antibody, anti-KIAA0261 antibody.

Specificity: ABIN151984 is specific for human WAPL protein. Species Reactivity: Human. Reactivity with Mouse WAPL is expected owing to complete identity betweenthis ortholog and Human WAPL at the site to which the epitope maps.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:2,500 - 1:10,000 Immunoprecipitation: not tested Histo/Cytochemistry: not tested*In some cases, the antibody may be diluted further than indicated.

Concentration: 1 mg/ml

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8. Preservative: 0.09% Sodium Azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Detection of Human WAPL by Western Blot. Sample: Nuclear extract (NE) from HeLa cells. Antibody: ABIN151984 used at 0.2 mcg/ml. Detection: Chemiluminescence with an exposure time of 20 minutes.