Breast Cancer 1 (BRCA1) antibody

Details for Product No. ABIN152030
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Antigen
Synonyms BRCA1
Reactivity
Human
(337), (45), (24), (13), (2), (1), (1), (1)
Host
Mouse
(296), (62), (3), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(14), (13), (11), (9), (9), (9), (9), (9), (9), (9), (9), (3), (3), (3)
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(195), (92), (90), (86), (69), (66), (50), (5), (5), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN152030
Quantity 0.1 mL
Price
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Immunogen Human BRCA1 (between residues 1314-1864).
Clone RAY
Isotype IgG
Specificity This antibody recognizes the epitope aa 1314-1600. Species Reactivity: This antibody reacts with human. Other species not tested. Localization: nuclear.
Cross-Reactivity Human
Purification Purified
Alternative Name BRCA1 / RNF53
Background BRCA1 (breast and ovarian cancer susceptibility protein 1) is a RING finger proteincontaining a BRCT domain. BRCA1 exists as a heterodimer with 22 possible isoforms.The full length protein has a reported molecular weight of 208 kD. BRCA1 localizes tothe mitotic spindle microtubules, centriole walls, pericentriolar fibers at centrosomes.Unphosphorylated BRCA1 localizes on chromosomes from metaphase throughtelophase, phosphorylated BRCA1 resides in inner chromosomal structure, centrosome,cleavage furrow during prophase through telophase, and relocalizes to the perinuclearregion when cells are subjected to IR or UV radiation in S phase. BRCA1 acts as atumor suppressor and can function as a secreted growth inhibitory protein, participate intranscription coupled repair of oxidative DNA damage, X-chromosome inactivation, andcan function as a E3 ubiquitin ligase. BRCA1 can be transcriptionally downregulated byEts-2, Brg-1, and Hmga-1. BRCA1 can be modified by glycosylation, ubiquitination andphosphorylation by CDK4, ATM/ATR, cdk2, and hChk2. The BRCA1 protein has beenreported to interact with RNA polymerase II holoenzyme and BARD1. BRCA1 containsat least two nuclear localization signals and is proposed to be a tumor suppressorprotein. It is a serine phosphoprotein that undergoes hyperphosphorylation during lateG1 and S phases of the cell cycle and is transiently dephosphorylated early after Mphase. BRCA1 protein alters in a qualitative and quantitative manner during cell cycleprogression. The amount of BRCA1 protein is highest during S phase and remainselevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA1function confers an increased susceptibility for both breast and ovarian cancer. Alternate Names: anti-Breast Cancer 1 antibody, anti-Breast cancer 1 early onset antibody, anti-Breastcancer type 1 susceptibility protein antibody, anti-Breast Ovarian Cancer Susceptibilityantibody, anti-Papillary serous carcinoma of the peritoneum antibody, anti-PSCPantibody, anti-RNF53 antibody.
Gene ID 672
UniProt P38398
Application Notes Suggested working dilutions: Western Blot 2-4 ug/ml
Restrictions For Research Use only
Format Liquid
Concentration 2.76 mg/ml
Buffer Preservative: Sodium Azide.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-Breast Cancer 1 (BRCA1) antibody Detection of BRCA1 in MCF-7 whole cell lysate using ABIN152030. 1 minute ECL detection.
anti-Breast Cancer 1 (BRCA1) antibody (2) Detection of BRCA1 using ABIN152030.
General Okada, Ouchi: "Cell cycle differences in DNA damage-induced BRCA1 phosphorylation affect its subcellular localization." in: The Journal of biological chemistry, Vol. 278, Issue 3, pp. 2015-20, 2003 (PubMed).

Hosts (296), (62), (3), (1)
Reactivities (337), (45), (24), (13), (2), (1), (1), (1)
Applications (195), (92), (90), (86), (69), (66), (50), (5), (5), (1), (1), (1)
Conjugates (14), (13), (11), (9), (9), (9), (9), (9), (9), (9), (9), (3), (3), (3)
Epitopes (41), (35), (24), (18), (16), (15), (14), (14), (13), (12), (10), (8), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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