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p300 antibody (E1A Binding Protein P300) (AA 1572-2371)

Details for Product anti-EP300 Antibody No. ABIN152048, Supplier: Log in to see
Antigen
  • A430090G16
  • A730011L11
  • KAT3B
  • p300
  • p300 HAT
  • RSTS2
Alternatives
anti-Human p300 antibody for Immunoprecipitation
Epitope
AA 1572-2371, AA 2107-2283
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2
2
1
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1
Reactivity
Human, Mouse (Murine), Mustelid, Primate, Rat (Rattus)
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114
102
37
36
14
3
2
2
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2
Host
Mouse
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110
Clonality (Clone)
Monoclonal ()
Conjugate
This p300 antibody is un-conjugated
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4
2
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2
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
187
99
79
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67
57
49
46
26
16
5
4
2
2
1
1
1
1
Supplier
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Immunogen Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
Clone RW109
Isotype IgG1 kappa
Specificity This is specific for p300 protein. This recognizes residues 2107-2283.
Purification Ascites
Alternative Name KAT3B/p300 (EP300 Antibody Abstract)
Background Gene Symbol: EP300
Molecular Weight Theoretical MW: 300 kDa
Gene ID 2033, 328572, 170915
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway
Application Notes Western Blot 1:1000, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunoprecipitation 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for KAT3B / p300 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 % Tris-acetate) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1.5 hours.
. Dilute the mouse anti-p300 primary antibody in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-p300 antibody (E1A Binding Protein P300) (AA 1572-2371) (ABIN152048) p300 detected in a HeLa nuclear extract using ABIN152048 (1:1,000). ECL: 20 minute e...
Product cited in: Romano, Staibano, Greco, Brunetti, Nappo, Ilardi, Martinelli, Sorrentino, Di Pace, Mascolo, Bisogni, Scalvenzi, Alfano, Romano: "FK506 binding protein 51 positively regulates melanoma stemness and metastatic potential." in: Cell death & disease, Vol. 4, pp. e578, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting,Chromatin Immunoprecipitation,Immunoprecipitation

Eckner, Ludlow, Lill, Oldread, Arany, Modjtahedi, DeCaprio, Livingston, Morgan: "Association of p300 and CBP with simian virus 40 large T antigen." in: Molecular and cellular biology, Vol. 16, Issue 7, pp. 3454-64, 1996 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunoprecipitation

Eckner, Yao, Oldread, Livingston: "Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation." in: Genes & development, Vol. 10, Issue 19, pp. 2478-90, 1996 (PubMed).

Eckner, Ewen, Newsome, Gerdes, DeCaprio, Lawrence, Livingston: "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor." in: Genes & development, Vol. 8, Issue 8, pp. 869-84, 1994 (PubMed). (Sample species: Human). Further details: Western Blotting