E1A Binding Protein P300 (EP300) (AA 1572-2371) antibody

Details for Product No. ABIN152048
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Antigen
Synonyms KAT3B, RSTS2, p300, A430090G16, A730011L11, p300 HAT
Epitope
AA 1572-2371
(32), (28), (19), (12), (11), (6), (5), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus), Mustelid
(179), (77), (76), (48), (48), (36), (24), (24), (16), (6)
Host
Mouse
(126), (58)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(9), (9), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2)
Application
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(105), (59), (41), (40), (38), (38), (38), (31), (10), (8), (6), (5), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 0.2 mL
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Catalog No. ABIN152048
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Immunogen Fusion protein containing residues 1572-2371 of human KAT3B/p300.
Clone RW109
Isotype IgG1 kappa
Specificity This is specific for p300 protein. This recognizes residues 2107-2283.
Purification Ascites
Alternative Name EP300 / P300
Background P300 is a relatively stable, ubiquitously expressed, nuclear phosphoprotein which isconserved among a variety of mammalian species. It is actively synthesized andphosphorylated in both quiescent and proliferating cells.Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) andp300 are closely related transcriptional coactivators and have been shown to directlyinteract with many different DNA-binding transcription factors including nuclear hormonereceptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Both CBP andp300 have been shown to display histone acetyltransferase (HAT) activity, capable ofacetylating all four core histone particles in nucleosomes. As a result of HAT activity, ithas been suggested CBP and p300 may play a direct role in activating chromatin fortranscription. Alternate Names: anti-CREBBP/EP300 inhibitory protein 1 antibody, anti-Cyclic AMP responsive enhancerbinding protein antibody, anti-EP300: E1A binding protein p300 antibody, anti-RB andP300 binding protein EID 1 antibody, anti-Retinoblastoma protein associated proteinantibody.
Gene Symbol: EP300
Gene ID 2033, 328572, 170915
Application Notes This KAT3B/p300 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot where a band is seen at ~300 kDa. A lower non-specific MW bands (~50 kDa) may be seen with longer exposure times. Chromatin Immunoprecipitation was reported in scientific literature.
Recommended dilutions: Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunoprecipitation 1:10-1:500, Western Blot 1:1000
Protocol Protocol specific for KAT3B / p300 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 % Tris-acetate) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1.5 hours.
. Dilute the mouse anti-p300 primary antibody in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-E1A Binding Protein P300 (EP300) (AA 1572-2371) antibody p300 detected in a HeLa nuclear extract using ABIN152048 (1:1,000). ECL: 20 minute exposure.
General Eckner, Ewen, Newsome et al.: "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor." in: Genes & development, Vol. 8, Issue 8, pp. 869-84, 1994 (PubMed).

Eckner, Ludlow, Lill et al.: "Association of p300 and CBP with simian virus 40 large T antigen." in: Molecular and cellular biology, Vol. 16, Issue 7, pp. 3454-64, 1996 (PubMed).

Eckner, Yao, Oldread et al.: "Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation." in: Genes & development, Vol. 10, Issue 19, pp. 2478-90, 1996 (PubMed).

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