VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (C-Term)

Details for Product anti-KDR Antibody No. ABIN152058, Supplier: Log in to see
Antigen
  • vegfr-2
  • KDR
  • 6130401C07
  • Flk-1
  • Flk1
  • Krd-1
  • Ly73
  • VEGFR-2
  • VEGFR2
  • sVEGFR-2
  • Vegfr-2
  • CD309
  • FLK1
  • VEGFR
  • FLK-1
  • flk-1
  • FLK/KDR
  • flk1
  • flk1b
  • kdrb
  • si:busm1-205d10.1
  • si:ch211-254j6.1
  • si:ch211-278f21.4
  • wu:fc31a09
  • fk52c05
  • flk
  • kdr
  • kdra
  • vegfr2
  • vegfr4
  • vegr2
  • wu:fk52c05
  • vascular endothelial growth factor receptor 2
  • kinase insert domain protein receptor
  • kinase insert domain receptor
  • kinase insert domain receptor (a type III receptor tyrosine kinase)
  • kinase insert domain receptor like
  • vascular endothelial growth factor receptor-2
  • vegfr-2
  • Kdr
  • KDR
  • kdr
  • kdrl
Alternatives
anti-Mouse (Murine) VEGFR2 antibody for Biochemical Assay
Epitope
C-Term, AA 1158-1365
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58
53
38
25
23
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18
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2
1
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1
1
1
1
1
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1
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1
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Reactivity
Human, Mouse (Murine)
611
371
183
25
7
4
4
4
3
3
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
534
141
60
4
2
2
1
Clonality
Polyclonal
Conjugate
This VEGFR2 antibody is un-conjugated
45
32
24
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18
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11
9
9
5
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5
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4
3
3
3
3
3
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3
3
2
2
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2
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
462
239
207
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118
108
40
33
26
24
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11
9
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3
2
1
1
1
1
1
1
1
Options
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Available images

Immunogen Residues corresponding to the kinase insert or C-terminus of mouse VEGF Receptor 2 (residues 1158-1365). [Swiss-Prot# P35918]
Purification Unpurified
Alternative Name CD309 / VEGFR-2 / Flk-1 (KDR Antibody Abstract)
Background Gene Symbol: KDR
Molecular Weight Theoretical MW: 150 kDa
Gene ID 3791
UniProt P35918
Pathways RTK Signaling, Glycosaminoglycan Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Regulation of long-term Neuronal Synaptic Plasticity
Application Notes Western Blot 1:2000-1:10000, Immunohistochemistry 1:10-1:500, Immunoprecipitation 2-5 μL, Immunohistochemistry-Paraffin 1:10-1:500This VEGF Receptor 2 antibody is useful for Immunohistochemistry paraffin-embedded sections (PMID 10751359), Immunoprecipitation and Western blot where a doublet can been seen at ~ 150 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for VEGF Receptor 2 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 %) on samples to be analyzed, loading 20 µg of total transfected protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS with 0.5 % BSA for 1 hour.
. Dilute the rabbit anti-VEGFR2 primary antibody in blocking buffer and incubate 1.5 hours at room temperature.
. Wash the membrane in water for 5 minutes and apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only
Format Liquid
Buffer Whole antisera
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (C-Term) (ABIN152058) anti-Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase) (KDR) (AA 1158-1365), (C-Term) antibody
Western Blotting (WB) image for anti-VEGFR2 antibody (Kinase insert Domain Receptor (A Type III Receptor tyrosine Kinase)) (C-Term) (ABIN152058) Western Blot: VEGF R2/KDR/Flk-1 Antibody [ABIN152058] - Analysis of VEGF R2/KDR/Flk-1...
Product cited in: Ying, Wang, Yang, Gu, Mai, Qiu, Chen, Wang: "Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway." in: Life sciences, Vol. 144, pp. 208-17, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Tsuboi, Yamashita, Nagano, Kimura, Toa Salazar, Ohneda: "Impaired expression of HIF-2α induces compensatory expression of HIF-1α for the recovery from anemia." in: Journal of cellular physiology, Vol. 230, Issue 7, pp. 1534-48, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

André, Tunik, Aronsson, Kvanta: "Hypoxia-Inducible Factor-1α Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model." in: Investigative ophthalmology & visual science, Vol. 56, Issue 11, pp. 6591-604, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Puglisi, Puppin, Pegolo, Andreetta, Pascoletti, DAurizio, Pandolfi, Fasola, Piga, Damante, Di Loreto: "Expression of periostin in human breast cancer." in: Journal of clinical pathology, Vol. 61, Issue 4, pp. 494-8, 2008 (PubMed).

Singh, Meyer, Band, Rahimi: "The carboxyl terminus of VEGFR-2 is required for PKC-mediated down-regulation." in: Molecular biology of the cell, Vol. 16, Issue 4, pp. 2106-18, 2005 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Meyer, Singh, Rahimi: "The carboxyl terminus controls ligand-dependent activation of VEGFR-2 and its signaling." in: The Journal of biological chemistry, Vol. 279, Issue 1, pp. 735-42, 2003 (PubMed). Further details: Western Blotting,Immunoprecipitation

Rahimi, Dayanir, Lashkari: "Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16986-92, 2000 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Lashkari, Hirose, Yazdany, McMeel, Kazlauskas, Rahimi: "Vascular endothelial growth factor and hepatocyte growth factor levels are differentially elevated in patients with advanced retinopathy of prematurity." in: The American journal of pathology, Vol. 156, Issue 4, pp. 1337-44, 2000 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)