Vascular Endothelial Growth Factor A (VEGFA) antibody

Details for Product No. ABIN152088
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Antigen
Synonyms vegf, vegfa, wu:fj82c06, VEGF, vegf-a, vpf, vefg, VEGF-A, VPF, eVEGF120, eVEGF164, MVCD1, Vegf, Vegf120, Vegf164, Vegf188, Vpf, VEGF164
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(309), (116), (102), (60), (59), (55), (37), (29), (3), (3), (2), (1)
Host
Mouse
(292), (92), (17), (5), (3), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(37), (12), (11), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (3), (3), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(305), (206), (100), (87), (49), (24), (24), (19), (18), (16), (11), (11), (8), (8), (5), (4), (2), (2), (2)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN152088
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Immunogen Recombinant VEGF 189 protein.
Clone VG1
Isotype IgG1 kappa
Specificity This VEGF Antibody (VG1) detects the 189, 165 and 121 isoforms of VEGF
Purification Protein G purified
Alternative Name VEGF-A
Background VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linkedglycoprotein involved in angiogenesis which promotes tumor progression andmetastasis. It exhibits potent mitogenic and permeability inducing properties specific forthe vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF 165 andVEGF 121, are secreted proteins and act as diffusible agents, whereas the larger two(VEGF 189 and VEGF 206) remain cell associated. Alternate Names: anti-Vascular Endothelial Growth Factor antibody, anti-Vascular Permeability Factorantibody, anti-VEGFA antibody, anti-VPF antibody.
Gene Symbol: VEGFA
Gene ID 7422, 22339, 83785
Research Area Cytokines, Growth Factors, Receptors, Immunology, Inflammation, Signaling
Application Notes This VEGF antibody is useful for Immunohistochemistry (frozen and paraffin-embedded sections), Western blot and Immunocytochemistry/Immunofluorescence. ELISA was reported in scientific literature. In IHC a dilution of 1:20-1:50 was used in an ABC method. However, depending on the staining conditions employed, we suggest that the final dilution should be determined by the user. We suggest an incubation period of 30-60 minutes at room temperature. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining.
Recommended dilutions: ELISA, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry 1:20-1:100, Immunohistochemistry-Frozen 1:20-1:100, Immunohistochemistry-Paraffin 1:20-1:100, Western Blot 1-2 µg/mL
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris-glycine, 150 mM NaCl pH 7.5, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Turley, Scott, Watts et al.: "Expression of VEGF in routinely fixed material using a new monoclonal antibody VG1." in: The Journal of pathology, Vol. 186, Issue 3, pp. 313-8, 1999 (PubMed).

Giatromanolaki, Koukourakis, Sivridis et al.: "DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer." in: The Journal of pathology, Vol. 200, Issue 2, pp. 222-8, 2003 (PubMed).

Ptaszynska, Pendrak, Bandle et al.: "Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells." in: Molecular cancer research : MCR, Vol. 6, Issue 3, pp. 352-63, 2008 (PubMed).

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