Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) antibody

Details for Product No. ABIN152096
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Antigen
Synonyms hnrpa1, TPT1P, HNRPA1, HNRNPA1, hnRNP A1, HNRPA1L3, hnRNP-A1, D15Ertd119e, Hdp, Hnrpa1, hnrnp-A1, ROA1
Reactivity
Human
(63), (25), (22), (3), (3), (2), (1), (1), (1)
Host
Mouse
(49), (19)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Microarray (MA)
(68), (46), (32), (27), (19), (16), (8), (2), (1)
Pubmed 3 references available
Quantity 0.05 mL
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Catalog No. ABIN152096
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Immunogen Fusion of mouse myeloma (SP2/O) and splenocytes from NZB mice immunized with purified human hnRNP-A1
Clone 4B10
Isotype IgG2a
Specificity Detects a band at approximately 32-35 kDa. This immunoprecipitates the hnRNP complex.
Cross-Reactivity (Details) Not yet tested in other species.
Purification Affinity purified
Alternative Name HnRNP core protein A1 / HNRNPA1
Background RNA polymerase II trans cripts in the nucleus are in complex with several proteins calledheterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important inbiological activities such as transcription, premRNA processing, cytoplasmic mRNAtranslation, and turnover. hnRNPs can be isolated either by immunoprecipitation or bysucrose gradient fractionation of cell extracts.2, 4, 5 Isolated hnRNPs consist of proteingroups named A to U and many of these protein groups consist of more than oneisoform. The major steady-state proteinsof the isolated hnRNP complex are A1, A2, B1, B2, C1, and C2, with a range ofmolecular weight starting with 34 kDa up to 43 kDa.2, 4, 5 hnRNP-A1 is important inpre-mRNA processing and inmRNA export from the nucleus. The protein binds to its RNA target through aconsensus RNA-binding site UAGGGU. The protein contains a 38-amino acid domaincalled M9, which is important for the interaction with the transportin protein andtherefore, for its import and export from the nucleus. RanGTP mediatesdissociation of hnRNP -A1 from transportin. hnRNP-A1 is ubiquitously expressed with ahigher expression in proliferating and/or transformed cells than in differentiated tissues.6. Alternate Names: anti-Helix destabilizing protein antibody, anti-Heterogeneous nuclear ribonucleoproteinA1 antibody, anti-Heterogeneous nuclear ribonucleoprotein A1B protein antibody,anti-Heterogeneous nuclear ribonucleoprotein B2 protein antibody, anti-Heterogeneousnuclear ribonucleoprotein core protein A1 antibody, anti-hnRNP core protein A1antibody, anti-Nuclear ribonucleoprotein particle A1 protein antibody, anti-Single strandDNA binding protein UP1 antibody, anti-syncrip antibody.
Gene Symbol: HNRNPA1
Gene ID 3178
Application Notes Recommended dilutions: Immunoprecipitation 1:10-1:500, Western Blot 0.25-0.5 µg/mL, ELISA Indirect, Microarray, Immunocytochemistry/Immunofluorescence 1:10-1:2000
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer 0.01 M PBS, pH 7.4, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Izaurralde, Jarmolowski, Beisel et al.: "A role for the M9 transport signal of hnRNP A1 in mRNA nuclear export." in: The Journal of cell biology, Vol. 137, Issue 1, pp. 27-35, 1997 (PubMed).

Siomi, Eder, Kataoka et al.: "Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins." in: The Journal of cell biology, Vol. 138, Issue 6, pp. 1181-92, 1997 (PubMed).

Vautier, Chesnue, Cunha et al.: "Transcription-dependent nucleocytoplasmic distribution of hnRNP A1 protein in early mouse embryos." in: Journal of cell science, Vol. 114, Issue Pt 8, pp. 1521-31, 2001 (PubMed).

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