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Keratin 19 (KRT19) (AA 350-400), (C-Term) antibody

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C-Term, AA 350-400
(70), (44), (21), (15), (13), (10), (10), (9), (8), (8), (8), (7), (7), (6), (6), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Monkey, Mouse (Murine), Rat (Rattus)
(534), (76), (52), (7), (7), (6), (4), (3), (3), (3), (2), (1), (1), (1)
(334), (205), (18), (5), (2)
(31), (18), (12), (6), (6), (6), (2), (2), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(373), (209), (205), (199), (100), (78), (64), (63), (55), (12), (10), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 0.1 mL
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Immunogen Synthetic peptide derived from the C-terminal region of human Cytokeratin 19 (between residues 350-400)
Purification Affinity purified
Alternative Name Cytokeratin 19 (KRT19 Antibody Abstract)
Background This protein is a member of the keratin family. The keratins are intermediate filamentproteins responsible for the structural integrity of epithelial cells and are subdivided intocytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins whichare arranged in pairs of heterotypic keratin chains. Unlike its related family members,this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelialcells. It is specifically expressed in the periderm, the transiently superficial layer thatenvelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes,therefore, antibody to keratin 19 is useful in the identification of liver metastasis. Thedegree of keratin 19 positivity in breast cancer distinguishes malignant from benigntumours. Keratin 19 is often co-expressed with keratin 7. Alternate Names: anti-ck19 antibody, anti-Cytokeratin 19 antibody, anti-k19 antibody, anti-k1cs antibody,anti-Keratin 19 antibody, anti-Keratin type i 40kD antibody, anti-krt19 antibody,anti-mgc15366 antibody.
Gene Symbol: KRT19
Gene ID 3880, 16669
UniProt P08727
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes This Cytokeratin 19 antibody is useful for Western Blot where a band is seen ~44 kDa, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry-paraffin sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:50-1:100, Immunohistochemistry 1:500, Immunohistochemistry-Paraffin 1:500, Western Blot 1:1000
Protocol Western Blot protocol specific for Cytokeratin 19 antibody
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunohistochemistry-Paraffin Embedded Sections Protocol Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.08 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Immunohistochemistry (IHC) image for anti-Keratin 19 (KRT19) (AA 350-400), (C-Term) antibody (ABIN152110) Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 19 an...
Product cited in: Takeda, Kojima, Ikejima et al.: "Death receptor 5 mediated-apoptosis contributes to cholestatic liver disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10895-900, 2008 (PubMed).

Catalog No. ABIN152110

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