Keratin 19 (KRT19) (AA 350-400), (C-Term) antibody

Details for Product No. ABIN152110, Supplier: Log in to see
Antigen
  • k19
  • ck19
  • k1cs
  • krt9
  • krt15
  • MGC76282
  • MGC83069
  • KRT19
  • CK19
  • K19
  • K1CS
  • GK-19
  • Ka19
  • Krt1-19
  • AI663979
  • EndoC
  • Krt-1.19
  • keratin 19
  • KRT19
  • krt19
  • Krt19
Epitope
C-Term, AA 350-400
133
76
48
34
22
19
16
15
12
10
9
8
8
8
7
7
6
6
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
851
191
134
19
18
15
11
9
7
5
5
4
3
3
2
2
1
1
1
1
1
1
Host
Rabbit
630
214
24
5
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
48
30
27
19
16
16
16
16
16
15
15
15
14
14
14
14
12
7
7
6
4
3
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
607
434
311
291
281
268
225
181
104
19
15
12
11
9
3
2
2
1
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen Synthetic peptide derived from the C-terminal region of human Cytokeratin 19 (between residues 350-400) [UniProt P08727]
Purification Immunogen affinity purified
Alternative Name Cytokeratin 19 (KRT19 Antibody Abstract)
Background Gene Symbol: KRT19
Gene ID 3880
UniProt P08727
Research Area Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes Western Blot 1:1000, Simple Western 1:1000, Immunohistochemistry 1:500, Immunocytochemistry/Immunofluorescence 1:50-1:100, Immunohistochemistry-Paraffin 1:500This Cytokeratin 19 antibody is useful for Western Blot where a band is seen ~44 kDa, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry-paraffin sections. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western Blot protocol specific for Cytokeratin 19 antibody
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.**Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.Immunohistochemistry-Paraffin Embedded Sections Protocol Antigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1.08 mg/mL
Buffer PBS and 30 % Glycerol
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-Keratin 19 (KRT19) (AA 350-400), (C-Term) antibody (ABIN152110) Formalin fixed paraffin embedded human colon carcinoma stained with Cytokeratin 19 an...
Immunohistochemistry (IHC) image for anti-Keratin 19 (KRT19) (AA 350-400), (C-Term) antibody (ABIN152110) Immunohistochemistry: Cytokeratin 19 Antibody [ABIN152110] - Staining of Cytokeratin ...
Western Blotting (WB) image for anti-Keratin 19 (KRT19) (AA 350-400), (C-Term) antibody (ABIN152110) Western Blot: Cytokeratin 19 Antibody [ABIN152110] - Analysis of Cytokeratin 19 in 1....
Simple Western (SimWes) image for anti-Keratin 19 (KRT19) (AA 350-400), (C-Term) antibody (ABIN152110) Simple Western: Cytokeratin 19 Antibody [ABIN152110] - Simple Western lane view shows...
Immunofluorescence (IF) image for anti-Keratin 19 (KRT19) (AA 350-400), (C-Term) antibody (ABIN152110) Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [ABIN152110] - MCF7 c...
Product cited in: Loforese, Malinka, Keogh, Baier, Simillion, Montani, Halazonetis, Candinas, Stroka: "Impaired liver regeneration in aged mice can be rescued by silencing Hippo core kinases MST1 and MST2." in: EMBO molecular medicine, Vol. 9, Issue 1, pp. 46-60, 2016 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC), Western Blotting (WB) (Sample species: Mouse (Murine)).

Sawitza, Kordes, Götze, Herebian, Häussinger: "Bile acids induce hepatic differentiation of mesenchymal stem cells." in: Scientific reports, Vol. 5, pp. 13320, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Kordes, Sawitza, Götze, Herebian, Häussinger: "Hepatic stellate cells contribute to progenitor cells and liver regeneration." in: The Journal of clinical investigation, Vol. 124, Issue 12, pp. 5503-15, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunohistochemistry

Takeda, Kojima, Ikejima, Harada, Yamashina, Okumura, Aoyama, Frese, Ikeda, Haynes, Cretney, Yagita, Sueyoshi, Sato, Nakanuma, Smyth, Okumura: "Death receptor 5 mediated-apoptosis contributes to cholestatic liver disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10895-900, 2008 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry