alpha Tubulin antibody (TUBA1)

Details for Product anti-TUBA1 Antibody No. ABIN152113, Supplier: Log in to see
Antigen
  • K-ALPHA-1
  • TUBA2
  • mec-12
  • tuba4
  • alpha tubulin
  • similar to alpha tubulin
  • tubulin-alpha
  • tubulin, alpha 1b
  • tubulin, alpha 3e
  • tubulin, alpha 4b
  • LOC100009327
  • RGD1560000
  • LOC100101229
  • TUBA1B
  • TUBA3E
  • tuba4b
Alternatives
anti-Human alpha Tubulin antibody for Western Blotting
Reactivity
Bird (Avian), Chicken, Chinese Hamster, Cow (Bovine), Dog (Canine), Fruit Fly (Drosophila melanogaster), Fungi (Fusarium), Guinea Pig, Hamster, Human, Monkey, Mouse (Murine), Parasite, Pig (Porcine), Primate, Rabbit, Rat (Rattus), Xenopus laevis, Yeast
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This alpha Tubulin antibody is un-conjugated
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Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunomicroscopy (IM), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen Native chicken brain microtubules.
Clone DM1A
Isotype IgG1 kappa
Specificity Does not cross-react with beta Tubulin.
Purification Protein G purified
Alternative Name alpha Tubulin (TUBA1 Antibody Abstract)
Background Gene Symbol: TUBA1A
Molecular Weight Theoretical MW: 50 kDa
Gene ID 7846
Research Area Signaling, Cytoskeleton
Pathways Microtubule Dynamics
Application Notes Western Blot 1:5000, Simple Western 1:50, Flow Cytometry 1 μg per million cells, Immunohistochemistry 1:100-1:500, Immunocytochemistry/Immunofluorescence 1:50000-1:100000, Immunoprecipitation 1:50-1:100, Immunohistochemistry-Paraffin 1:100-1:500, Immunohistochemistry-Frozen 1:100-1:500, Immunomicroscopy, Immunofluorescence, CyTOF-readyThis alpha Tubulin Antibody (DM1A) is useful as a loading control for Western blot as well as Immunoprecipitation, Immunohistochemistry on paraffin-embedded and frozen sections, Immunocytochemistry/Immunofluorescence and Flow Cytometry. The DM1A alpha tubulin antibody is ideal for use as a Western blot loading control, where a band can be seen around 50-55 kDa and as a cytoskeletal marker in ICC. For IHC-Paraffin, antigen retrieval is not essential, but may optimize staining. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunohistochemistry (IHC) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Formalin fixed paraffin embedded human lung stained with Tubulin alpha antibody.
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Western Blot: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analsis of alpha tubulin i...
Immunohistochemistry (IHC) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunohistochemistry: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of paraff...
Immunohistochemistry (IHC) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunohistochemistry: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of formal...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [ABIN152113] - IHC analy...
Simple Western (SimWes) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Simple Western: alpha Tubulin Antibody (DM1A) [ABIN152113] - Simple Western lane view...
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Western Blot: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of HeLa and COS-7...
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Western Blot: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of alpha Tubulin ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [ABIN152113] - IHC analy...
Flow Cytometry (FACS) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Flow Cytometry: alpha Tubulin Antibody (DM1A) [ABIN152113] - Intracellular flow cytom...
Flow Cytometry (FACS) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Flow Cytometry: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of PE conjugate...
Flow Cytometry (FACS) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Flow Cytometry: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of PE conjugate...
Immunohistochemistry (IHC) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunohistochemistry: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of colon ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [ABIN152113] - IHC analy...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunohistochemistry-Paraffin: alpha Tubulin Antibody (DM1A) [ABIN152113] - IHC analy...
Immunohistochemistry (IHC) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunohistochemistry: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of formal...
Immunohistochemistry (IHC) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunohistochemistry: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of small ...
Western Blotting (WB) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Western Blot: alpha Tubulin Antibody (DM1A) [ABIN152113] - Western blot analysis of e...
Immunomicroscopy (IM) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunomicroscopy: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of HeLa cells...
Immunomicroscopy (IM) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunomicroscopy: alpha Tubulin Antibody (DM1A) [ABIN152113] - Staining of the marine...
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunofluorescence: alpha Tubulin Antibody (DM1A) [ABIN152113] - Staining of skin fib...
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [ABIN152113] - ...
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [ABIN152113] - ...
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (DM1A) [ABIN152113] - ...
Immunofluorescence (IF) image for anti-alpha Tubulin (TUBA1) antibody (ABIN152113) Immunofluorescence: alpha Tubulin Antibody (DM1A) [ABIN152113] - Analysis of embryoni...
Product cited in: Chen, Hsieh, Kuo, Kao, Hsu, Wang: "Water-soluble chitosan inhibits nerve growth factor and attenuates allergic inflammation in mite allergen-induced allergic rhinitis." in: The Journal of allergy and clinical immunology, 2017 (PubMed). Method employed by authors: Western Blotting (WB)

Hsuan, Chyau, Hung, Chen, Chou: "The induction of apoptosis and autophagy by Wasabia japonica extract in colon cancer." in: European journal of nutrition, Vol. 55, Issue 2, pp. 491-503, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

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Wang, Lin, Lo, Tang, Teng, Chao, Wu, Liu, Hsieh: "Oncogenic roles of carbonic anhydrase 8 in human osteosarcoma cells." in: Tumour biology, Vol. 37, Issue 6, pp. 7989-8005, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Park, Jang, Ko, Woo, Kim, Kim, Park, Park, Koh, Lee: "Statins Increase Mitochondrial and Peroxisomal Fatty Acid Oxidation in the Liver and Prevent Non-Alcoholic Steatohepatitis in Mice." in: Diabetes & metabolism journal, Vol. 40, Issue 5, pp. 376-385, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Macedo, Napimoga, Rocha-Neto, Abdalla, Clemente-Napimoga: "The role of endogenous opioid peptides in the antinociceptive effect of 15-deoxy(Δ12,14)-prostaglandin J2 in the temporomandibular joint." in: Prostaglandins, leukotrienes, and essential fatty acids, Vol. 110, pp. 27-34, 2016 (PubMed). Further details: Western Blotting

Chang, Hsu, Weng, Chen, Wang, Chou, Liu, Yuan, Huang, Lin, Chen, Tsai: "N-Glycosylation of Human R-Spondin 1 Is Required for Efficient Secretion and Stability but Not for Its Heparin Binding Ability." in: International journal of molecular sciences, Vol. 17, Issue 6, 2016 (PubMed). Further details: Western Blotting

Nakamura, Nishikawa, Saito, Sakai, Sasaki, Hashimoto, Okamoto, Suehiro, Yamasaki, Sakaida: "Decitabine inhibits tumor cell proliferation and up-regulates e-cadherin expression in Epstein-Barr virus-associated gastric cancer." in: Journal of medical virology, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Rueli, Torres, Dewing, Kiyohara, Barayuga, Bellinger, Uyehara-Lock, White, Moreira, Berry, Perry, Bellinger: "Selenoprotein S Reduces Endoplasmic Reticulum Stress-Induced Phosphorylation of Tau: Potential Role in Selenate Mitigation of Tau Pathology." in: Journal of Alzheimer's disease : JAD, Vol. 55, Issue 2, pp. 749-762, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Sidoli, Musner, Silvestri, Ungaro, DAntonio, Cavener, Feltri, Wrabetz: "Ablation of Perk in Schwann Cells Improves Myelination in the S63del Charcot-Marie-Tooth 1B Mouse." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 44, pp. 11350-11361, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Tu, Yu, Lee, Kuo, Lu, Tu, Chang: "Hinokitiol inhibits vasculogenic mimicry activity of breast cancer stem/progenitor cells through proteasome-mediated degradation of epidermal growth factor receptor." in: Oncology letters, Vol. 11, Issue 4, pp. 2934-2940, 2016 (PubMed).

Yu, Jia, Dong, Zhang, Xiao, Chen, Wang, Li, Wang, Zhang, Li: "Candida albicans autophagy, no longer a bystander: Its role in tolerance to ER stress-related antifungal drugs." in: Fungal genetics and biology : FG & B, Vol. 81, pp. 238-49, 2015 (PubMed). (Sample species: Fungi (Fusarium)). Further details: Western Blotting

Xu, Dong, Yu, Zhang, Zhang, Jia, Chen, Zhang, Xing, Li: "Convergent Regulation of Candida albicans Aft2 and Czf1 in Invasive and Opaque Filamentation." in: Journal of cellular biochemistry, Vol. 116, Issue 9, pp. 1908-18, 2015 (PubMed). (Sample species: Fungi (Fusarium)). Further details: Western Blotting

Bercht Pfleghaar, Taimen, Butin-Israeli, Shimi, Langer-Freitag, Markaki, Goldman, Wehnert, Goldman: "Gene-rich chromosomal regions are preferentially localized in the lamin B deficient nuclear blebs of atypical progeria cells." in: Nucleus (Austin, Tex.), Vol. 6, Issue 1, pp. 66-76, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Eriksen, Torgersen, Holm, Abrahamsen, Spurkland, Moskaug, Simonsen, Blomhoff: "Retinoic acid-induced IgG production in TLR-activated human primary B cells involves ULK1-mediated autophagy." in: Autophagy, Vol. 11, Issue 3, pp. 460-71, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Muñoz-Sáez, de Munck García, Arahuetes Portero, Vicente, Ortiz-López, Cantizani, Gómez Miguel: "Neuroprotective role of sphingosine-1-phosphate in L-BMAA treated neuroblastoma cells (SH-SY5Y)." in: Neuroscience letters, Vol. 593, pp. 83-9, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Stander, Stander, Joubert: "Synergistic anticancer potential of dichloroacetate and estradiol analogue exerting their effect via ROS-JNK-Bcl-2-mediated signalling pathways." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 35, Issue 4, pp. 1499-526, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Sulaiman, Chu, Blanco, Vallabhapurapu, Franco, Qi: "SapC-DOPS nanovesicles induce Smac- and Bax-dependent apoptosis through mitochondrial activation in neuroblastomas." in: Molecular cancer, Vol. 14, pp. 78, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

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