Podocalyxin-Like (PODXL) antibody

Details for Product No. ABIN152150, Supplier: Log in to see
Antigen
  • PODXL
  • podxl
  • fc18d02
  • wu:fc18d02
  • Gp200
  • PC
  • PCLP
  • PCLP-1
  • AW121214
  • Ly102
  • Pclp1
  • podocalyxin
  • Gp135
  • PCLP1
  • cPCLP1
  • MEP21
  • Podxl
  • podocalyxin-like
  • si:dkey-146n1.1
  • podocalyxin-like protein 1
  • PODXL
  • si:dkey-146n1.1
  • LOC100614602
  • Podxl
  • LOC100512201
  • PCLP1
Alternatives
anti-Human Podocalyxin-Like antibody for BioImaging
Reactivity
Human
308
94
82
61
2
2
2
1
1
Host
Mouse
183
120
24
20
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
20
19
16
14
12
12
10
9
8
7
7
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6
5
5
5
4
3
3
3
3
3
2
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
208
185
96
95
88
73
53
40
36
5
5
4
3
2
2
1
Options
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Immunogen Human embryonal carcinoma cell line 2102Ep.
Clone TRA-1-60
Isotype IgM
Specificity Specific for the neuraminidase resistant form of the TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. TRA-1-60 reacts with a sialidase-sensitive epitope.
No Cross-Reactivity Mouse (Murine)
Purification IgM purified
Alternative Name TRA-1-60 (PODXL Antibody Abstract)
Pathways Tube Formation
Application Notes Western Blot 1:100-1:2000, Flow Cytometry 1:50-1:200, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500This TRA-1-60 (R) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western blot.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol Specific for NB100-730 Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN152150) Western blot analysis of ABIN152150 expression in NTERA-2 cl.D1 whole cell lysate.
Flow Cytometry (FACS) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN152150) Flow Cytometry: TRA-1-60 (R) Antibody (TRA-1-60(R)) [ABIN152150] - Staining of NTERA-...
Western Blotting (WB) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN152150) Western Blot: TRA-1-60 (R) Antibody (TRA-1-60(R)) [ABIN152150] - Whole cell protein f...
Immunofluorescence (IF) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN152150) Immunocytochemistry/Immunofluorescence: TRA-1-60 (R) Antibody (TRA-1-60(R)) [ABIN1521...
Immunofluorescence (IF) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN152150) Immunocytochemistry/Immunofluorescence: TRA-1-60 (R) Antibody (TRA-1-60(R)) [ABIN1521...
Product cited in: Tang, Partyka, Hsueh, Sinha, Kletter, Zeh, Huang, Brand, Haab: "Glycans related to the CA19-9 antigen are elevated in distinct subsets of pancreatic cancers and improve diagnostic accuracy over CA19-9." in: Cellular and molecular gastroenterology and hepatology, Vol. 2, Issue 2, pp. 201-221.e15, 2016 (PubMed).

Rossbach, Hildebrand, El-Ahmad, Stachelscheid, Reinke, Kurtz: "Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector." in: Stem cell research, Vol. 16, Issue 2, pp. 314-7, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Matin, Walsh, Gokhale, Draper, Bahrami, Morton, Moore, Andrews: "Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells." in: Stem cells (Dayton, Ohio), Vol. 22, Issue 5, pp. 659-68, 2004 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Flow Cytometry,Immunofluorescence

Badcock, Pigott, Goepel, Andrews: "The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan." in: Cancer research, Vol. 59, Issue 18, pp. 4715-9, 1999 (PubMed). (Sample species: Human). Further details: Western Blotting